Global to push GA events into
skip to main content

Title: Extraction of solubles from plant biomass for use as microbial growth stimulant and methods related thereto

A method for producing a microbial growth stimulant (MGS) from a plant biomass is described. In one embodiment, an ammonium hydroxide solution is used to extract a solution of proteins and ammonia from the biomass. Some of the proteins and ammonia are separated from the extracted solution to provide the MGS solution. The removed ammonia can be recycled and the proteins are useful as animal feeds. In one embodiment, the method comprises extracting solubles from pretreated lignocellulosic biomass with a cellulase enzyme-producing growth medium (such T. reesei) in the presence of water and an aqueous extract.
Issue Date:
OSTI Identifier:
Patent Number(s):
Application Number:
Contract Number:
Resource Relation:
Patent File Date: 2010 Apr 19
Research Org:
Sponsoring Org:
Country of Publication:
United States

Other works cited in this record:

Process for Recovering Carbon Dioxide from a Gas Mixture
patent, October 1935

Adsorption Process and Apparatus
patent, April 1951

Process for the production of ammoniated straw and other plant materials containing lignocellulose
patent, December 1977

Ammonia absorber
patent, May 1979

Method of making compost and spawned compost, mushroom spawn and generating methane gas
patent, April 1981

Process for treating alfalfa and other cellulosic agricultural crops
patent, October 1982

Process for enriching the protein content of food and feedstuff and products thereof
patent, January 1983

Method for increasing the accessibility of cellulose in lignocellulosic materials, particularly hardwoods agricultural residues and the like
patent, July 1984

Bioconversion of agricultural wastes into proteinaceous animal feed
patent, July 1985

Process for separating carbonic acid gas from methane-rich gas
patent, April 1986

Apparatus for treating cellulose-containing matter, e.g. straw, with gaseous ammonia
patent, May 1986

Process for removing ammonia and acid gases from process streams
patent, June 1986

Method for increasing the reactivity and digestibility of cellulose with ammonia
patent, July 1986

Supercritical ammonia treatment of lignocellulosic materials
patent, February 1987

Composting process for the production of mushroom cultivation substrates
patent, July 1989

Process for separating and recovering carbonic acid gas from gas mixture by adsorption
patent, January 1991

Separation of gas from solvent by membrane technology
patent, February 1991

Continuous constant pressure staging of solid-vapor compound reactors
patent, June 1991

Process for increasing the reactivity of cellulose-containing materials
patent, August 1991

Integrated process for the production of food, feed and fuel from biomass
patent, September 1991

Ammonia recovery from purge gas
patent, May 1992

Biomass refining process
patent, December 1992

Treatment of fibrous lignocellulosic biomass by high shear forces in a turbulent couette flow to make the biomass more susceptible to hydrolysis
patent, December 1994

Process for the treatment of cellulose
patent, December 1995

Process for separating selected components from multi-component natural gas streams
patent, August 1997

Stabilization of biowastes
patent, April 1998

Methods of biomass pretreatment
patent, February 1999

Process for activating polysaccharides, polysaccharides produced by this process, and use thereof
patent, August 1999

Method for removing ammonia and carbon dioxide gases from a steam
patent, February 2000

Process for treating cellulosic materials
patent, August 2000

Apparatus for treating cellulosic materials
patent, January 2001

Microbial polyunsaturated fatty acid containing oil from pasteurised biomass
patent, July 2001

Apparatus, process and pressure reactor for the treatment of solids with pressurized liquid gases
patent, July 2002

Process for the production of hydrogen using a carbonated adsorbent with selected dubinin parameters
patent, July 2002

Process for the production of nutritional products with microorganisms using sequential solid substrate and liquid fermentation
patent, September 2002

Recirculating composting system
patent, February 2003

Recovery method of ammonia from gaseous mixture
patent, July 2003

Cellulose production from lignocellulosic biomass
patent, September 2003

Method for removing ammonia from a gas comprising hydrogen cyanide, ammonia and water
patent, March 2005

Low operating pressure gas scrubber
patent, May 2005

Transgenic plants containing ligninase and cellulase which degrade lignin and cellulose to fermentable sugars
patent, May 2006

NMR probe circuit with nodal impedance bridge
patent, March 2007

Process for separating nitrogen from methane using microchannel process technology
patent, July 2007

Transgenic plants containing ligninase and cellulase which degrade lignin and cellulose to fermentable sugars
patent, May 2008

Process for the production of animal feed and ethanol and novel animal feed
patent, February 2009

Process for the production of cellulolytic and hemicellulolytic enzymes using distillation residues from the ethanolic fermentation of enzymatic hydrolyzates of (ligno)cellulosic materials
patent, February 2009

Recovery of inorganic salt during processing of lignocellulosic feedstocks
patent, September 2009

Method of pre-treating woodchips prior to mechanical pulping
patent, August 2010

Integration of alternative feedstreams for biomass treatment and utilization
patent, March 2011

Method for producing water-absorbing polymer particles
patent, March 2011

Process for the treatment of lignocellulosic biomass
patent, March 2011

Process for treatment of biomass feedstocks
patent, May 2011

AG-wafer product and method of making same
patent, September 2011

Biomass pretreatment
patent, October 2011

Process for producing sugars and ethanol using corn stillage
patent, February 2013

Method of separating components from a gas stream
patent, March 2013

Process for the treatment of lignocellulosic biomass
patent, March 2013

Apparatus for pretreatment for saccharification of lignocellulose biomass
patent, April 2013

Lignocellulosic biomass saccharification pre-treatment device
patent, May 2013

Process using agriculture residue biomass for producing feed pellets
patent, October 2013

Anhydrous ammonia treatment for improved milling of biomass
patent, February 2014

Pretreated densified biomass products
patent, March 2014

Process for the treatment of lignocellulosic biomass
patent, July 2014

Biomass pelletizing process
patent, September 2014

Treatment of biomass to obtain fermentable sugars
patent-application, February 2007

Integration of alternative feedstreams for biomass treatment and utilization
patent-application, February 2007

Production of beta-glucosidase, hemicellulase and ligninase in E1 and FLC-cellulase-transgenic plants
patent-application, August 2007

Methods And Systems For Producing Ethanol Using Raw Starch And Fractionation
patent-application, August 2007

Process for the treatment of lignocellulosic biomass
patent-application, January 2008

Integrated process for separation of lignocellulosic components to fermentable sugars for production of ethanol and chemicals
patent-application, March 2008

Method And Apparatus For Extracting Carbon Dioxide From Air
patent-application, April 2008

System And Process For Producing Synthetic Liquid Hydrocarbon
patent-application, May 2008

Fuel pellet
patent-application, July 2008

System And Methods For Continuous Biomass Processing
patent-application, September 2008

Two-Stage Method For Pretreatment Of Lignocellulosic Biomass
patent-application, November 2010

Methods For Pretreating Biomass
patent-application, August 2011

Method Of Separating Components From A Gas Stream
patent-application, December 2011

Pretreated Densified Biomass Products And Methods Of Making And Using Same
patent-application, December 2011

Method For Converting Lignocellulosic Biomass
patent-application, March 2012

Methods And Component Parts For Manufacturing Foam Paint Rollers
patent-application, March 2012

Methods For Producing High-Freeness Pulp
patent-application, April 2012

Continuously Fed Biomass Pretreatment Process For A Packed Bed Reactor
patent-application, May 2012

Biomass Pretreatment Process For A Packed Bed Reactor
patent-application, May 2012

Integrated Processes For Conversion Of Lignocellulosic Biomass To Bioproducts And Systems And Apparatus Related Thereto
patent-application, August 2014

Increasing nutrient availability of feather meal for ruminants and non-ruminants using an ammonia pressurisation/depressurisation process
journal, May 1999

Fungal Upgrading of Wheat Straw for Straw-Thermoplastics Production
journal, January 2004
  • Houghton, Tracy P.; Thompson, David N.; Hess, J. Richard
  • Applied Biochemistry and Biotechnology, Vol. 113, Issue 1-3, p. 71-93
  • DOI: 10.1385/ABAB:113:1-3:071

Botanical fractions of rice straw colonized by white-rot fungi: changes in chemical composition and structure
journal, October 1995
  • Karunanandaa, K.; Varga, G. A.; Akin, D. E.
  • Animal Feed Science and Technology, Vol. 55, Issue 3-4, p. 179-199
  • DOI: 10.1016/0377-8401(95)00805-W

Composting of a crop residue through treatment with microorganisms and subsequent vermicomposting
journal, November 2002

Optimization of the ammonia fiber explosion (AFEX) treatment parameters for enzymatic hydrolysis of corn stover
journal, December 2005
  • Teymouri, Farzaneh; Laureano-Perez, Lizbeth; Alizadeh, Hasan
  • Bioresource Technology, Vol. 96, Issue 18, p. 2014-2018
  • DOI: 10.1016/j.biortech.2005.01.016

An initial assessment of spent mushroom compost as a potential energy feedstock
journal, September 2001

Comparative sugar recovery data from laboratory scale application of leading pretreatment technologies to corn stover
journal, December 2005

Coordinated development of leading biomass pretreatment technologies
journal, December 2005

Oyster mushroom cultivation with rice and wheat straw
journal, May 2002

Roll Press Briquetting and Pelleting of Corn Stover and Switchgrass
journal, January 2009
  • Kaliyan, N.; Morey, R. V.; White, M. D.
  • Transactions of the ASABE, Vol. 52, Issue 2, p. 543-555
  • DOI: 10.13031/2013.26812

Production of Cellulase by Solid-State Fermentation
book, January 1999
  • Cen, Peilin; Xia, Liming; Scheper, T.
  • Recent Progress in Bioconversion of Lignocellulosics
  • DOI: 10.1007/3-540-49194-5_4

Production of Cellulase in Solid-State Fermentation with Trichoderma reesei MCG 80 on Wheat Straw
book, January 1996
  • Chahal, Parminder S.; Chahal, Devinder S.; Lê, George B. B.
  • Seventeenth Symposium on Biotechnology for Fuels and Chemicals
  • DOI: 10.1007/978-1-4612-0223-3_40

Microbial Cellulose Utilization: Fundamentals and Biotechnology
journal, September 2002
  • Lynd, L. R.; Weimer, P. J.; van Zyl, W. H.
  • Microbiology and Molecular Biology Reviews, Vol. 66, Issue 3, p. 506-577
  • DOI: 10.1128/MMBR.66.3.506-577.2002

Advancement and comparative profiles in the production technologies using solid-state and submerged fermentation for microbial cellulases
journal, June 2010
  • Singhania, Reeta Rani; Sukumaran, Rajeev K.; Patel, Anil Kumar
  • Enzyme and Microbial Technology, Vol. 46, Issue 7, p. 541-549
  • DOI: 10.1016/j.enzmictec.2010.03.010

Production and characterization of cellulolytic enzymes from Trichoderma reesei grown on various carbon sources
journal, January 1992
  • Warzywoda, Michel; Larbre, Elisabeth; Pourquié, Jacques
  • Bioresource Technology, Vol. 39, Issue 2, p. 125-130
  • DOI: 10.1016/0960-8524(92)90130-P

Process and economic analysis of pretreatment technologies
journal, December 2005

In vitro and in vivo digestibility of soya-bean straw treated with various alkalis
journal, August 1990

journal, April 2006
  • Gray, Kevin A.; Zhao, Lishan; Emptage, Mark
  • Current Opinion in Chemical Biology, Vol. 10, Issue 2, p. 141-146
  • DOI: 10.1016/j.cbpa.2006.02.035

Bio-ethanol – the fuel of tomorrow from the residues of today
journal, December 2006
  • Hahn-Hägerdal, B.; Galbe, M.; Gorwa-Grauslund, M. F.
  • Trends in Biotechnology, Vol. 24, Issue 12, p. 549-556
  • DOI: 10.1016/j.tibtech.2006.10.004

Features of promising technologies for pretreatment of lignocellulosic biomass
journal, April 2005

The Path Forward for Biofuels and Biomaterials
journal, January 2006
  • Ragauskas, Arthur J.; Williams, Charlotte K.; Davison, Brian H.
  • Science, Vol. 311, Issue 5760, p. 484-489
  • DOI: 10.1126/science.1114736

Hydrolysis of lignocellulosic materials for ethanol production: a review
journal, May 2002

Improving Digestibility of Straws for Ruminant Feed by Aqueous Ammonia
journal, July 1972
  • Waiss, A. C.; Guggolz, J.; Kohler, G. O.
  • Journal of Animal Science, Vol. 35, Issue 1, p. 109-112
  • DOI: 10.2527/jas1972.351109x

Toward an aggregated understanding of enzymatic hydrolysis of cellulose: Noncomplexed cellulase systems
journal, November 2004
  • Zhang, Yi-Heng Percival; Lynd, Lee R.
  • Biotechnology and Bioengineering, Vol. 88, Issue 7, p. 797-824
  • DOI: 10.1002/bit.20282

Assessment of the influence of media particle size on the biofiltration of odorous exhaust ventilation air from a piggery facility
journal, September 2002

Biological waste air treatment in biofilters
journal, June 1997

Effect of particle size based separation of milled corn stover on AFEX pretreatment and enzymatic digestibility
journal, January 2006
  • Chundawat, Shishir P.S.; Venkatesh, Balan; Dale, Bruce E.
  • Biotechnology and Bioengineering, Vol. 96, Issue 2, p. 219-231
  • DOI: 10.1002/bit.21132

Replacement of fish meal in diets for Australian silver perch, Bidyanus bidyanus: I. Digestibility of alternative ingredients
journal, June 2000

Assays for Total Protein
book, September 1995

Obtaining a protein concentrate from integral defatted sunflower flour
journal, June 2001

Switchgrass as a sustainable bioenergy crop
journal, April 1996

Cellulase production using biomass feed stock and its application in lignocellulose saccharification for bio-ethanol production
journal, February 2009
  • Sukumaran, Rajeev K.; Singhania, Reeta Rani; Mathew, Gincy Marina
  • Renewable Energy, Vol. 34, Issue 2, p. 421-424
  • DOI: 10.1016/j.renene.2008.05.008

Induction and catabolite repression mechanisms of cellulase in fungi
journal, January 2001

Two-step SSCF to convert AFEX-treated switchgrass to ethanol using commercial enzymes and Saccharomyces cerevisiae 424A(LNH-ST)
journal, November 2010

Lime pretreatment and enzymatic hydrolysis of corn stover
journal, December 2005

Pretreatment and fractionation of corn stover by ammonia recycle percolation process
journal, December 2005

Pretreatment of Corn Stover by Low-Liquid Ammonia Recycle Percolation Process
journal, January 2006
  • Kim, Tae Hyun; Lee, Yoon Y.; Sunwoo, Changshin
  • Applied Biochemistry and Biotechnology, Vol. 133, Issue 1, p. 41-58
  • DOI: 10.1385/ABAB:133:1:41

Does densification influence the steam pretreatment and enzymatic hydrolysis of softwoods to sugars?
journal, October 2012

Cellulosic ethanol production from AFEX-treated corn stover using Saccharomyces cerevisiae 424A(LNH-ST)
journal, January 2009
  • Lau, M. W.; Dale, B. E.
  • Proceedings of the National Academy of Sciences, Vol. 106, Issue 5, p. 1368-1373
  • DOI: 10.1073/pnas.0812364106

Partial flow of compressed-hot water through corn stover to enhance hemicellulose sugar recovery and enzymatic digestibility of cellulose
journal, December 2005

Complete Rations for Dairy Cattle. II. Sugarcane Bagasse Pellets as Roughage in Blended Rations for Lactating Cows
journal, June 1975

Optimization of pH controlled liquid hot water pretreatment of corn stover
journal, December 2005

Detoxification methods of aflatoxins. A review
journal, May 1995

Combined effect of pelleting and pretreatment on enzymatic hydrolysis of switchgrass
journal, July 2012

Complete Rations for Dairy Cattle. V. Interaction of Sugarcane Bagasse Quantity and Form with Soybean Meal, Urea, and Starea
journal, September 1975

Recent process improvements for the ammonia fiber expansion (AFEX) process and resulting reductions in minimum ethanol selling price
journal, November 2008

Combined sugar yields for dilute sulfuric acid pretreatment of corn stover followed by enzymatic hydrolysis of the remaining solids
journal, December 2005

Effects of the pelleting conditions on chemical composition and sugar yield of corn stover, big bluestem, wheat straw, and sorghum stalk pellets
journal, October 2011
  • Theerarattananoon, Karnnalin; Xu, Feng; Wilson, Jonathan
  • Bioprocess and Biosystems Engineering, Vol. 35, Issue 4, p. 615-623
  • DOI: 10.1007/s00449-011-0642-8

The effect of different treatment conditions on biomass binder preparation for lignite briquette
journal, November 2001

Lignocellulosic Biomass Pretreatment Using AFEX
book, January 2009

Evaluating the impact of ammonia fiber expansion (AFEX) pretreatment conditions on the cost of ethanol production
journal, January 2011

Growth of the plant cell wall
journal, November 2005
  • Cosgrove, Daniel J.
  • Nature Reviews Molecular Cell Biology, Vol. 6, Issue 11, p. 850-861
  • DOI: 10.1038/nrm1746

Mixture optimization of six core glycosyl hydrolases for maximizing saccharification of ammonia fiber expansion (AFEX) pretreated corn stover
journal, April 2010
  • Gao, Dahai; Chundawat, Shishir P. S.; Krishnan, Chandraraj
  • Bioresource Technology, Vol. 101, Issue 8, p. 2770-2781
  • DOI: 10.1016/j.biortech.2009.10.056

Separation of Glucose and Pentose Sugars by Selective Enzyme Hydrolysis of AFEX-Treated Corn Fiber
book, January 2007
  • Hanchar, Robert J.; Teymouri, Farzaneh; Nielson, Chandra D.
  • Applied Biochemistry and Biotecnology
  • p. 313-325
  • DOI: 10.1007/978-1-60327-181-3_28

Optimization of enzymatic hydrolysis and ethanol fermentation from AFEX-treated rice straw
journal, April 2009
  • Zhong, Cheng; Lau, Ming W.; Balan, Venkatesh
  • Applied Microbiology and Biotechnology, Vol. 84, Issue 4, p. 667-676
  • DOI: 10.1007/s00253-009-2001-0

Multi-scale visualization and characterization of lignocellulosic plant cell wall deconstruction during thermochemical pretreatment
journal, January 2011
  • Chundawat, Shishir P. S.; Donohoe, Bryon S.; da Costa Sousa, Leonardo
  • Energy & Environmental Science, Vol. 4, Issue 3, 973
  • DOI: 10.1039/C0EE00574F

Similar records in DOepatents and OSTI.GOV collections: