Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control

Cary, Robert E.

Title: Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control

Abstract

Highly simplified lateral flow chromatographic nucleic acid sample preparation methods, devices, and integrated systems are provided for the efficient concentration of trace samples and the removal of nucleic acid amplification inhibitors. Methods for capturing and reducing inhibitors of nucleic acid amplification reactions, such as humic acid, using polyvinylpyrrolidone treated elements of the lateral flow device are also provided. Further provided are passive fluid control methods and systems for use in lateral flow assays.

Inventors:: Cary, Robert E.

Issue Date:: 2015-12-08

Research Org.:: Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1228041

Patent Number(s):: 9207236

Application Number:: 12/940,973

Assignee:: Los Alamos National Security, LLC

Patent Classifications (CPCs):: G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

Show more

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N1/405 - {by adsorption or absorption}

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/101 - {by chromatography, e.g. electrophoresis, ion-exchange, reverse phase}

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N33/558 - using diffusion or migration of antigen or antibody

Show less

DOE Contract Number:: AC52-06NA25396

Resource Type:: Patent

Resource Relation:: Patent File Date: 2010 Nov 05

Country of Publication:: United States

Language:: English

Subject:: 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats


                    Cary, Robert E. Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control.  United States: N. p., 2015. 
        Web.

Copy to clipboard


                    Cary, Robert E. Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control.  United States.

Copy to clipboard


                    Cary, Robert E. Tue .  
        "Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control".  United States.  https://www.osti.gov/servlets/purl/1228041.

Copy to clipboard


                    
@article{osti_1228041,

  title        = {Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control},

  author       = {Cary, Robert E.},

  abstractNote = {Highly simplified lateral flow chromatographic nucleic acid sample preparation methods, devices, and integrated systems are provided for the efficient concentration of trace samples and the removal of nucleic acid amplification inhibitors. Methods for capturing and reducing inhibitors of nucleic acid amplification reactions, such as humic acid, using polyvinylpyrrolidone treated elements of the lateral flow device are also provided. Further provided are passive fluid control methods and systems for use in lateral flow assays.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2015},

  month        = {12}

}

Copy to clipboard

Patent:

Save / Share:

Export Metadata

Save to My Library

Works referenced in this record:

Biochemical analysis of components of the pre-replication complex of Archaeoglobus fulgidus
journal, August 2003

Grainge, I.
Nucleic Acids Research, Vol. 31, Issue 16
https://doi.org/10.1093/nar/gkg662

One-step immobilization of poly(dT)-modified DNA onto non-modified plastic substrates by UV irradiation for microarrays
journal, August 2006

Kimura, Naoki
Biochemical and Biophysical Research Communications, Vol. 347, Issue 2, p. 477-484
https://doi.org/10.1016/j.bbrc.2006.06.130

Ligation mediated PCR performed at low denaturation temperatures--PCR melting profiles
journal, September 2003

Masny, A.
Nucleic Acids Research, Vol. 31, Issue 18
https://doi.org/10.1093/nar/gng116

Characterization of a Thermostable UvrD Helicase and Its Participation in Helicase-dependent Amplification
journal, June 2005

An, Lixin; Tang, Wen; Ranalli, Tamara A.
Journal of Biological Chemistry, Vol. 280, Issue 32, p. 28952-28958
https://doi.org/10.1074/jbc.M503096200

Shape matters
journal, March 2003

Buhro, William E.; Colvin, Vicki L.
Nature Materials, Vol. 2, Issue 3
https://doi.org/10.1038/nmat844

Genetic Relationship among Worldwide Strains of Xanthomonas Causing Canker in Citrus Species and Design of New Primers for Their Identification by PCR
journal, March 2002

Cubero, J.; Graham, J. H.
Applied and Environmental Microbiology, Vol. 68, Issue 3
https://doi.org/10.1128/AEM.68.3.1257-1264.2002

Simultaneous Visual Detection of Multiple Viral Amplicons by Dipstick Assay
journal, August 2005

Dineva, M. A.; Candotti, D.; Fletcher-Brown, F.
Journal of Clinical Microbiology, Vol. 43, Issue 8
https://doi.org/10.1128/JCM.43.8.4015-4021.2005

Lab-on-a-chip devices for global health: Past studies and future opportunities
journal, January 2007

Chin, Curtis D.; Linder, Vincent; Sia, Samuel K.
Lab Chip, Vol. 7, Issue 1
https://doi.org/10.1039/B611455E

Signal amplification through nucleotide extension and excision on a dendritic DNA platform
journal, April 2000

Capaldi, S.
Nucleic Acids Research, Vol. 28, Issue 7
https://doi.org/10.1093/nar/28.7.e21

Accessing Single Nucleotide Polymorphisms in Genomic DNA by Direct Multiplex Polymerase Chain Reaction Amplification on Oligonucleotide Microarrays
journal, April 2002

Huber, Martin; Mündlein, Axel; Dornstauder, Eva
Analytical Biochemistry, Vol. 303, Issue 1
https://doi.org/10.1006/abio.2001.5565

Rapid, Sensitive, and Specific Lateral-Flow Immunochromatographic Device To Measure Anti-Anthrax Protective Antigen Immunoglobulin G in Serum and Whole Blood
journal, May 2006

Biagini, R. E.; Sammons, D. L.; Smith, J. P.
Clinical and Vaccine Immunology, Vol. 13, Issue 5
https://doi.org/10.1128/CVI.13.5.541-546.2006

Transmission of the Citrus Variegated Chlorosis Bacterium Xylella fastidiosa with the Sharpshooter Oncometopia nigricans
journal, November 2002

Brlansky, R. H.; Damsteegt, V. D.; Hartung, J. S.
Plant Disease, Vol. 86, Issue 11
https://doi.org/10.1094/PDIS.2002.86.11.1237

Helicase-dependent isothermal DNA amplification
journal, August 2004

Vincent, Myriam; Xu, Yan; Kong, Huimin
EMBO reports, Vol. 5, Issue 8
https://doi.org/10.1038/sj.embor.7400200

Genetic Diversity of Pierce's Disease Strains and Other Pathotypes of Xylella fastidiosa
journal, February 2001

Hendson, M.; Purcell, A. H.; Chen, D.
Applied and Environmental Microbiology, Vol. 67, Issue 2
https://doi.org/10.1128/AEM.67.2.895-903.2001

Direct fluorescence analysis of genetic polymorphisms by hybridization with oligonucleotide arrays on glass supports
journal, January 1994

Guo, Zhen; Guilfoyle, Richard A.; Thiel, Andrew J.
Nucleic Acids Research, Vol. 22, Issue 24
https://doi.org/10.1093/nar/22.24.5456

A Universal Nucleic Acid Sequence Biosensor with Nanomolar Detection Limits
journal, February 2004

Baeumner, Antje J.; Pretz, Jennifer; Fang, Shirley
Analytical Chemistry, Vol. 76, Issue 4
https://doi.org/10.1021/ac034945l

Populations of Xylella fastidiosa in Plants Required for Transmission by an Efficient Vector
journal, December 1997

Hill, B. L.; Purcell, A. H.
Phytopathology, Vol. 87, Issue 12
https://doi.org/10.1094/PHYTO.1997.87.12.1197

Isothermal reactions for the amplification of oligonucleotides
journal, April 2003

Van Ness, J.; Van Ness, L. K.; Galas, D. J.
Proceedings of the National Academy of Sciences, Vol. 100, Issue 8, p. 4504-4509
https://doi.org/10.1073/pnas.0730811100

Development of immunocapture reverse transcription loop-mediated isothermal amplification for the detection of tomato spotted wilt virus from chrysanthemum
journal, October 2004

Fukuta, Shiro; Ohishi, Kazushi; Yoshida, Keiko
Journal of Virological Methods, Vol. 121, Issue 1, p. 49-55
https://doi.org/10.1016/j.jviromet.2004.05.016

Biosensors for environmental pollutants and food contaminants
journal, October 2003

Baeumner, Antje J.
Analytical and Bioanalytical Chemistry, Vol. 377, Issue 3
https://doi.org/10.1007/s00216-003-2158-9

Capture of Single-Stranded DNA Assisted by Oligonucleotide Modules
journal, January 1998

O'Meara, Deirdre; Nilsson, Peter; Nygren, Per-Åke
Analytical Biochemistry, Vol. 255, Issue 2
https://doi.org/10.1006/abio.1997.2472

Strand displacement amplification—an isothermal, in vitro DNA amplification technique
journal, January 1992

Walker, G. Terrance; Fraiser, Melinda S.; Schram, James L.
Nucleic Acids Research, Vol. 20, Issue 7, p. 1691-1696
https://doi.org/10.1093/nar/20.7.1691

A highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus detection
journal, September 1992

Wetzel, T.; Candresse, T.; Macquaire, G.
Journal of Virological Methods, Vol. 39, Issue 1-2, p. 27-37
https://doi.org/10.1016/0166-0934(92)90122-T

Early diagnosis of bubonic plague using F1 antigen capture ELISA assay and rapid immunogold dipstick
journal, July 2000

Chanteau, Suzanne; Rahalison, Lila; Ratsitorahina, Mahery
International Journal of Medical Microbiology, Vol. 290, Issue 3, p. 279-283
https://doi.org/10.1016/S1438-4221(00)80126-5

Advances in Polymerase Chain Reaction on Microfluidic Chips
journal, June 2005

Roper, Michael G.; Easley, Christopher J.; Landers, James P.
Analytical Chemistry, Vol. 77, Issue 12
https://doi.org/10.1021/ac050756m

Helicase structure and mechanism
journal, February 2002

Caruthers, Jonathan M.; McKay, David B.
Current Opinion in Structural Biology, Vol. 12, Issue 1, p. 123-133
https://doi.org/10.1016/S0959-440X(02)00298-1

Fluorescent Amplified Fragment Length Polymorphism Analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis Isolates
journal, February 2004

Hill, K. K.; Ticknor, L. O.; Okinaka, R. T.
Applied and Environmental Microbiology, Vol. 70, Issue 2
https://doi.org/10.1128/AEM.70.2.1068-1080.2004

DnaB from Thermus aquaticus Unwinds Forked Duplex DNA with an Asymmetric Tail Length Dependence
journal, March 1999

Kaplan, Daniel L.; Steitz, Thomas A.
Journal of Biological Chemistry, Vol. 274, Issue 11
https://doi.org/10.1074/jbc.274.11.6889

LNA (locked nucleic acids): synthesis and high-affinity nucleic acid recognition
journal, January 1998

Singh, Sanjay K.; Koshkin, Alexei A.; Wengel, Jesper
Chemical Communications, Issue 4
https://doi.org/10.1039/A708608C

Development of a multiplex immunocapture RT-PCR assay for detection and differentiation of tomato and tobacco mosaic tobamoviruses
journal, October 1998

Jacobi, V.; Bachand, G. D.; Hamelin, R. C.
Journal of Virological Methods, Vol. 74, Issue 2, p. 167-178
https://doi.org/10.1016/S0166-0934(98)00086-X

Direct isolation of poly(A)+ RNA from 4 M guanidine thiocyanate-lysed cell extracts using locked nucleic acid-oligo(T) capture
journal, April 2004

Jacobsen, N.
Nucleic Acids Research, Vol. 32, Issue 7
https://doi.org/10.1093/nar/gnh056

Potent and nontoxic antisense oligonucleotides containing locked nucleic acids
journal, May 2000

Wahlestedt, C.; Salmi, P.; Good, L.
Proceedings of the National Academy of Sciences, Vol. 97, Issue 10
https://doi.org/10.1073/pnas.97.10.5633

The total influenza vaccine failure of 1947 revisited: Major intrasubtypic antigenic change can explain failure of vaccine in a post-World War II epidemic
journal, July 2002

Kilbourne, E. D.; Smith, C.; Brett, I.
Proceedings of the National Academy of Sciences, Vol. 99, Issue 16, p. 10748-10752
https://doi.org/10.1073/pnas.162366899

Detection and Diversity Assessment of Xylella fastidiosa in Field-Collected Plant and Insect Samples by Using 16S rRNA and gyrB Sequences
journal, July 2003

Rodrigues, J. L. M.; Silva-Stenico, M. E.; Gomes, J. E.
Applied and Environmental Microbiology, Vol. 69, Issue 7
https://doi.org/10.1128/AEM.69.7.4249-4255.2003

Pierce's Disease of Grapevines: Isolation of the Causal Bacterium
journal, January 1978

Davis, M. J.; Purcell, A. H.; Thomson, S. V.
Science, Vol. 199, Issue 4324
https://doi.org/10.1126/science.199.4324.75

Microarray technology – an intellectual property retrospective
journal, September 2003

Rouse, Richard; Hardiman, Gary
Pharmacogenomics, Vol. 4, Issue 5
https://doi.org/10.1517/phgs.4.5.623.23792

Multi-analyte single-membrane biosensor for the serotype-specific detection of Dengue virus
journal, July 2004

Zaytseva, NatalyaV.; Montagna, RichardA.; Lee, EunMi
Analytical and Bioanalytical Chemistry, Vol. 380, Issue 1
https://doi.org/10.1007/s00216-004-2724-9

An efficient and economic enhancer mix for PCR
journal, September 2006

Ralser, Markus; Querfurth, Robert; Warnatz, Hans-Jörg
Biochemical and Biophysical Research Communications, Vol. 347, Issue 3, p. 747-751
https://doi.org/10.1016/j.bbrc.2006.06.151

Design, biochemical, biophysical and biological properties of cooperative antisense oligonucleotides
journal, January 1995

Kandimalla, Ekambar R.; Manning, Adrienne; Lathan, Christopher
Nucleic Acids Research, Vol. 23, Issue 17
https://doi.org/10.1093/nar/23.17.3578

Chemiluminescence Detection for Hybridization Assays on the Flow-Thru Chip, a Three-Dimensional Microchannel Biochip
journal, December 2001

Cheek, Brady J.; Steel, Adam B.; Torres, Matthew P.
Analytical Chemistry, Vol. 73, Issue 24
https://doi.org/10.1021/ac0108616

Multiplex diagnostic platforms for detection of biothreat agents
journal, November 2004

Cirino, Nick M.; Musser, Kimberlee A.; Egan, Christina
Expert Review of Molecular Diagnostics, Vol. 4, Issue 6
https://doi.org/10.1586/14737159.4.6.841

High-performance membrane chromatography: Highly efficient separation method for proteins in ion-exchange, hydrophobic interaction and reversed-phase modes
journal, September 1993

Tennikova, Tatiana B.; Svec, Frantisek
Journal of Chromatography A, Vol. 646, Issue 2, p. 279-288
https://doi.org/10.1016/0021-9673(93)83340-X

Identification of A/H5N1 Influenza Viruses Using a Single Gene Diagnostic Microarray
journal, January 2007

Dawson, Erica D.; Moore, Chad L.; Dankbar, Daniela M.
Analytical Chemistry, Vol. 79, Issue 1
https://doi.org/10.1021/ac061920o

Using a microfluidic device for 1 l DNA microarray hybridization in 500 s
journal, April 2005

Wei, C. -W.
Nucleic Acids Research, Vol. 33, Issue 8
https://doi.org/10.1093/nar/gni078

Incidence of adamantane resistance among influenza A (H3N2) viruses isolated worldwide from 1994 to 2005: a cause for concern
journal, October 2005

Bright, Rick A.; Medina, Marie-jo; Xu, Xiyan
The Lancet, Vol. 366, Issue 9492
https://doi.org/10.1016/S0140-6736(05)67338-2

Optimization of DNA-tagged dye-encapsulating liposomes for lateral-flow assays based on sandwich hybridization
journal, August 2006

Edwards, Katie A.; Baeumner, Antje J.
Analytical and Bioanalytical Chemistry, Vol. 386, Issue 5
https://doi.org/10.1007/s00216-006-0705-x

Lateral flow microarrays: a novel platform for rapid nucleic acid detection based on miniaturized lateral flow chromatography
journal, April 2007

Carter, D. J.; Cary, R. B.
Nucleic Acids Research, Vol. 35, Issue 10
https://doi.org/10.1093/nar/gkm269

Development of a Novel, Rapid Integrated Cryptosporidium parvum Detection Assay
journal, July 2000

Kozwich, D.; Johansen, K. A.; Landau, K.
Applied and Environmental Microbiology, Vol. 66, Issue 7
https://doi.org/10.1128/AEM.66.7.2711-2717.2000

Specific PCR detection and identification of Xylella fastidiosa strains causing citrus variegated chlorosis
journal, December 1995

Pooler, Margaret R.; Hartung, John S.
Current Microbiology, Vol. 31, Issue 6
https://doi.org/10.1007/BF00294703

Membrane chromatography - an integrative concept in the downstream processing of proteins
journal, July 1995

Thoemmes, J.; Kula, M. -R.
Biotechnology Progress, Vol. 11, Issue 4
https://doi.org/10.1021/bp00034a001

Direct detection of potato leafroll virus in potato tubers by immunocapture and the isothermal nucleic acid amplification method NASBA
journal, June 1997

Leone, G.; van Schijndel, H. B.; van Genien, B.
Journal of Virological Methods, Vol. 66, Issue 1, p. 19-27
https://doi.org/10.1016/S0166-0934(97)02203-9

Chromatographic performance of a thin microporous bed of nitrocellulose
journal, November 2001

Lönnberg, Maria; Carlsson, Jan
Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 763, Issue 1-2, p. 107-120
https://doi.org/10.1016/S0378-4347(01)00376-0

The ligase chain reaction in a PCR world.
journal, August 1991

Barany, F.
Genome Research, Vol. 1, Issue 1
https://doi.org/10.1101/gr.1.1.5

An array of opportunities
journal, April 2002

Gershon, Diane
Nature, Vol. 416, Issue 6883
https://doi.org/10.1038/416885a

Biosensor for the specific detection of a single viable B. anthracis spore
journal, May 2003

Hartley, Harriet A.; Baeumner, Antje J.
Analytical and Bioanalytical Chemistry, Vol. 376, Issue 3
https://doi.org/10.1007/s00216-003-1939-5

Biochemical Characterization of the DNA Helicase Activity of the Escherichia coli RecQ Helicase
journal, October 2000

Harmon, Frank G.; Kowalczykowski, Stephen C.
Journal of Biological Chemistry, Vol. 276, Issue 1
https://doi.org/10.1074/jbc.M006555200

Isolation, Culture, and Pathogenicity of the Bacterium Causing Phony Disease of Peach
journal, January 1983

Wells, John M.
Phytopathology, Vol. 73, Issue 6
https://doi.org/10.1094/Phyto-73-859

A novel rapid hybridization technique: paper chromatography hybridization assay (PACHA)
journal, December 1993

Reinhartz, Avraham; Alajem, Sara; Samson, Aline
Gene, Vol. 136, Issue 1-2, p. 221-226
https://doi.org/10.1016/0378-1119(93)90468-I

Rapid, direct extraction of DNA from soils for PCR analysis using polyvinylpolypyrrolidone spin columns
journal, April 1996

Berthelet, Marc; Whyte, Lyle G.; Greer, Charles W.
FEMS Microbiology Letters, Vol. 138, Issue 1, p. 17-22
https://doi.org/10.1111/j.1574-6968.1996.tb08128.x

A nested-PCR assay for detection of Xylella fastidiosa in citrus plants and sharpshooter leafhoppers
journal, March 2004

Ciapina, L. P.; Carareto Alves, L. M.; Lemos, E. G. M.
Journal of Applied Microbiology, Vol. 96, Issue 3
https://doi.org/10.1111/j.1365-2672.2004.02176.x

Developing rapid, point-of-care, multiplex detection for use in lateral flow devices
conference, November 2005

Rao, R. S.; Albala, J. S.; Lane, S. L.
Optics East 2005, SPIE Proceedings
https://doi.org/10.1117/12.630157

Dependence of the melting temperature of DNA on salt concentration
journal, April 1965

Schildkraut, Carl; Lifson, Shneior
Biopolymers, Vol. 3, Issue 2
https://doi.org/10.1002/bip.360030207

Influenza vaccines: present and future
journal, July 2002

Palese, Peter; García-Sastre, Adolfo
Journal of Clinical Investigation, Vol. 110, Issue 1
https://doi.org/10.1172/JCI15999

Current and Developing Technologies for Monitoring Agents of Bioterrorism and Biowarfare
journal, October 2005

Lim, D. V.; Simpson, J. M.; Kearns, E. A.
Clinical Microbiology Reviews, Vol. 18, Issue 4
https://doi.org/10.1128/CMR.18.4.583-607.2005

DNA Detection Using Recombination Proteins
journal, June 2006

Piepenburg, Olaf; Williams, Colin H.; Stemple, Derek L.
PLoS Biology, Vol. 4, Issue 7
https://doi.org/10.1371/journal.pbio.0040204

Microchromatography and microelectrophoresis on nitrocellulose membranes
journal, May 1970

Přistoupil, T. I.
Chromatographic Reviews, Vol. 12, Issue 2, p. 109-125
https://doi.org/10.1016/0009-5907(70)80007-2

Purification and characterization of Thermus thermophilus UvrD
journal, February 2003

Collins, Ruairi; McCarthy, Tommie V.
Extremophiles, Vol. 7, Issue 1
https://doi.org/10.1007/s00792-002-0293-4

Fate of Pierce's Disease Strains of Xylella fastidiosa in Common Riparian Plants in California
journal, September 1999

Purcell, A. H.; Saunders, S. R.
Plant Disease, Vol. 83, Issue 9
https://doi.org/10.1094/PDIS.1999.83.9.825

Development of a Novel One-Tube Isothermal Reverse Transcription Thermophilic Helicase-Dependent Amplification Platform for Rapid RNA Detection
journal, November 2007

Goldmeyer, James; Kong, Huimin; Tang, Wen
The Journal of Molecular Diagnostics, Vol. 9, Issue 5
https://doi.org/10.2353/jmoldx.2007.070012

Use of laser microdissection greatly improves the recovery of DNA from sperm on microscope slides
journal, October 2003

Elliott, K.; Hill, D. S.; Lambert, C.
Forensic Science International, Vol. 137, Issue 1, p. 28-36
https://doi.org/10.1016/S0379-0738(03)00267-6

Microfluidic Device for Rapid (<15 min) Automated Microarray Hybridization
journal, October 2005

Peytavi, R.
Clinical Chemistry, Vol. 51, Issue 10
https://doi.org/10.1373/clinchem.2005.052845

Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
journal, January 1988

Saiki, R. K.; Gelfand, D. H.; Stoffel, S.
Science, Vol. 239, Issue 4839, p. 487-491
https://doi.org/10.1126/science.2448875

The thermodynamic advantage of DNA oligonucleotide 'stacking hybridization' reactions: energetics of a DNA nick
journal, February 1997

Lane, M.
Nucleic Acids Research, Vol. 25, Issue 3
https://doi.org/10.1093/nar/25.3.611

One step visual detection of PCR products with gold nanoparticles and a nucleic acid lateral flow (NALF) device
journal, January 2007

Aveyard, Jenny; Mehrabi, Maryam; Cossins, Andrew
Chemical Communications, Issue 41
https://doi.org/10.1039/B708859K

Detection of amantadine-resistant variants among avian influenza viruses isolated in North America and Asia
journal, October 2005

Ilyushina, Natalia A.; Govorkova, Elena A.; Webster, Robert G.
Virology, Vol. 341, Issue 1, p. 102-106
https://doi.org/10.1016/j.virol.2005.07.003

Molecular Crowding Regulates the Structural Switch of the DNA G-Quadruplex ^†
journal, December 2002

Miyoshi, Daisuke; Nakao, Akihiro; Sugimoto, Naoki
Biochemistry, Vol. 41, Issue 50
https://doi.org/10.1021/bi020412f

Detection of Xylella fastidiosa in potential insect vectors by immunomagnetic separation and nested polymerase chain reaction
journal, August 1997

Pooler, M. R.; Myung, I. S.; Bentz, J.
Letters in Applied Microbiology, Vol. 25, Issue 2
https://doi.org/10.1046/j.1472-765X.1997.00188.x

Optimal conditions for hybridization with oligonucleotides: A study with myc-oncogene DNA probes
journal, April 1988

Albretsen, Catrine; Haukanes, Bjørn-Ivar; Aasland, Rein
Analytical Biochemistry, Vol. 170, Issue 1, p. 193-202
https://doi.org/10.1016/0003-2697(88)90108-X

Identification of the Heme Compound Copurified with Deoxyribonucleic Acid (DNA) from Bloodstains, a Major Inhibitor of Polymerase Chain Reaction (PCR) Amplification
journal, March 1994

Akane, Atsushi; Matsubara, Kazuo; Nakamura, Hiroaki
Journal of Forensic Sciences, Vol. 39, Issue 2
https://doi.org/10.1520/JFS13607J

Biosensor for Dengue Virus Detection: Sensitive, Rapid, and Serotype Specific
journal, March 2002

Baeumner, Antje J.; Schlesinger, Nicole A.; Slutzki, Naomi S.
Analytical Chemistry, Vol. 74, Issue 6
https://doi.org/10.1021/ac015675e

RNA replication: required intermediates and the dissociation of template, product, and Q.beta. replicase
journal, May 1979

Dobkin, Carl; Mills, Donald R.; Kramer, Fred Russell
Biochemistry, Vol. 18, Issue 10
https://doi.org/10.1021/bi00577a030

Evaluation of NucliSens easyMAG for Automated Nucleic Acid Extraction from Various Clinical Specimens
journal, December 2006

Loens, K.; Bergs, K.; Ursi, D.
Journal of Clinical Microbiology, Vol. 45, Issue 2
https://doi.org/10.1128/JCM.00894-06

Detection of Cell and Tissue Surface Antigens Using Up-Converting Phosphors: A New Reporter Technology
journal, February 1999

Zijlmans, H. J. M. A. A.; Bonnet, J.; Burton, J.
Analytical Biochemistry, Vol. 267, Issue 1
https://doi.org/10.1006/abio.1998.2965

Encoded evidence: DNA in forensic analysis
journal, October 2004

Jobling, Mark A.; Gill, Peter
Nature Reviews Genetics, Vol. 5, Issue 10
https://doi.org/10.1038/nrg1455

Detection of foodborne pathogens using DNA probes and a dipstick format
journal, December 1996

Groody, E. Patrick
Molecular Biotechnology, Vol. 6, Issue 3
https://doi.org/10.1007/BF02761710

Nucleic acid sequence-based amplification
journal, March 1991

Compton, J.
Nature, Vol. 350, Issue 6313
https://doi.org/10.1038/350091a0

Recovery and detection of enterovirus, hepatitis A virus and Norwalk virus in hardshell clams (Mercenaria mercenaria) by RT-PCR methods
journal, February 1999

Suñén, Ester; Sobsey, Mark D.
Journal of Virological Methods, Vol. 77, Issue 2, p. 179-187
https://doi.org/10.1016/S0166-0934(98)00148-7

Immobilization of polynucleotides on magnetic particles. Factors influencing hybridization efficiency
journal, September 1991

Day, P. J.; Flora, P. S.; Fox, J. E.
Biochemical Journal, Vol. 278, Issue 3
https://doi.org/10.1042/bj2780735

Inclusion of polyvinylpyrrolidone in the polymerase chain reaction reverses the inhibitory effects of polyphenolic contamination of RNA
journal, February 1999

Koonjul, P. K.; Brandt, W. F.; Lindsey, G. G.
Nucleic Acids Research, Vol. 27, Issue 3
https://doi.org/10.1093/nar/27.3.915

Use of Single Nucleotide Polymorphisms in the plcR Gene for Specific Identification of Bacillus anthracis
journal, April 2005

Easterday, W. R.; Van Ert, M. N.; Simonson, T. S.
Journal of Clinical Microbiology, Vol. 43, Issue 4
https://doi.org/10.1128/JCM.43.4.1995-1997.2005

Synthesis and hybridization of a series of biotinylated oligonucleotides
journal, January 1988

Cook, Alan F.; Vuocolo, Edmund; Brakel, Christine L.
Nucleic Acids Research, Vol. 16, Issue 9
https://doi.org/10.1093/nar/16.9.4077

A rapid biosensor for viable B. anthracis spores
journal, August 2004

Baeumner, AntjeJ.; Leonard, Barbara; McElwee, John
Analytical and Bioanalytical Chemistry, Vol. 380, Issue 1
https://doi.org/10.1007/s00216-004-2726-7

Diagnostic applications of microarrays
journal, August 2006

Petrik, J.
Transfusion Medicine, Vol. 16, Issue 4
https://doi.org/10.1111/j.1365-3148.2006.00673.x

Point-of-care diagnostics: will the hurdles be overcome this time?
journal, July 2006

Huckle, David
Expert Review of Medical Devices, Vol. 3, Issue 4
https://doi.org/10.1586/17434440.3.4.421

An investigation of the rigor of interpretation rules for STRs derived from less than 100 pg of DNA
journal, July 2000

Gill, Peter; Whitaker, Jonathan; Flaxman, Christine
Forensic Science International, Vol. 112, Issue 1, p. 17-40
https://doi.org/10.1016/S0379-0738(00)00158-4

Association of a Rickettsialike Organism with Pierce's Disease of Grapevines and Alfalfa Dwarf and Heat Therapy of the Disease in Grapevines
journal, January 1973

Goheen, A. C.
Phytopathology, Vol. 63, Issue 3
https://doi.org/10.1094/Phyto-63-341

Rapid single-tube immunocapture RT-PCR for the detection of two yam potyviruses
journal, December 1997

Mumford, R. A.; Seal, S. E.
Journal of Virological Methods, Vol. 69, Issue 1-2, p. 73-79
https://doi.org/10.1016/S0166-0934(97)00141-9

A System for Specific, High-throughput Genotyping by Allele-specific Primer Extension on Microarrays
journal, July 2000

Pastinen, T.
Genome Research, Vol. 10, Issue 7
https://doi.org/10.1101/gr.10.7.1031

Characteristics and Applications of Nucleic Acid Sequence-Based Amplification (NASBA)
journal, January 2002

Deiman, Birgit; van Aarle, Pierre; Sillekens, Peter
Molecular Biotechnology, Vol. 20, Issue 2
https://doi.org/10.1385/MB:20:2:163

DNA analysis with multiplex microarray-enhanced PCR
journal, January 2005

Pemov, A.
Nucleic Acids Research, Vol. 33, Issue 2
https://doi.org/10.1093/nar/gnh184

Real-time PCR in the microbiology laboratory
journal, March 2004

Mackay, I. M.
Clinical Microbiology and Infection, Vol. 10, Issue 3
https://doi.org/10.1111/j.1198-743X.2004.00722.x

Influenza
journal, November 2003

Nicholson, Karl G.; Wood, John M.; Zambon, Maria
The Lancet, Vol. 362, Issue 9397
https://doi.org/10.1016/S0140-6736(03)14854-4

Technology platforms for pharmacogenomic diagnostic assays
journal, September 2004

Koch, Walter H.
Nature Reviews Drug Discovery, Vol. 3, Issue 9
https://doi.org/10.1038/nrd1496

Culture and serological detection of the xylem-limited bacterium causing citrus variegated chlorosis and its identification as a strain ofXylella fastidiosa
journal, September 1993

Chang, Chung J.; Garnier, Monique; Zreik, Leyla
Current Microbiology, Vol. 27, Issue 3
https://doi.org/10.1007/BF01576010

Amplification and cloning of a long RNA virus genome using immunocapture-long RT-PCR
journal, June 1997

Romero, Alicia; Blanco-Urgoiti, Begoña; Ponz, Fernando
Journal of Virological Methods, Vol. 66, Issue 1, p. 159-163
https://doi.org/10.1016/S0166-0934(97)00043-8

Thermodynamic effects of formamide on DNA stability
journal, June 1996

Blake, R.
Nucleic Acids Research, Vol. 24, Issue 11
https://doi.org/10.1093/nar/24.11.2095

PCR-based diagnostics for infectious diseases: uses, limitations, and future applications in acute-care settings
journal, June 2004

Yang, Samuel; Rothman, Richard E.
The Lancet Infectious Diseases, Vol. 4, Issue 6, p. 337-348
https://doi.org/10.1016/S1473-3099(04)01044-8

Virulence signatures: microarray-based approaches to discovery and analysis
journal, November 2004

Pannucci, James; Cai, Hong; Pardington, Paige E.
Biosensors and Bioelectronics, Vol. 20, Issue 4, p. 706-718
https://doi.org/10.1016/j.bios.2004.04.005

NASBA^TM isothermal enzymatic in vitro nucleic acid amplification optimized for the diagnosis of HIV-1 infection
journal, December 1991

Kievits, Tim; van Gemen, Bob; van Strijp, Dianne
Journal of Virological Methods, Vol. 35, Issue 3, p. 273-286
https://doi.org/10.1016/0166-0934(91)90069-C

Pierce's Disease Bacterium: Mechanism of Transmission by Leafhopper Vectors
journal, November 1979

Purcell, A. H.; Finlay, A. H.; Mclean, D. L.
Science, Vol. 206, Issue 4420
https://doi.org/10.1126/science.206.4420.839

A Bead-Based Method for Multiplexed Identification and Quantitation of DNA Sequences Using Flow Cytometry
journal, October 2000

Spiro, A.; Lowe, M.; Brown, D.
Applied and Environmental Microbiology, Vol. 66, Issue 10
https://doi.org/10.1128/AEM.66.10.4258-4265.2000

Locked nucleic acid (LNA): fine-tuning the recognition of DNA and RNA
journal, January 2001

Braasch, Dwaine A.; Corey, David R.
Chemistry & Biology, Vol. 8, Issue 1, p. 1-7
https://doi.org/10.1016/S1074-5521(00)00058-2

Colorimetric detection of immobilised PCR products generated on a solid support
journal, March 1997

Lockley, A.
Nucleic Acids Research, Vol. 25, Issue 6
https://doi.org/10.1093/nar/25.6.1313

Quantitative PCR Method for Diagnosis of Citrus Bacterial Canker
journal, June 2001

Cubero, J.; Graham, J. H.; Gottwald, T. R.
Applied and Environmental Microbiology, Vol. 67, Issue 6
https://doi.org/10.1128/AEM.67.6.2849-2852.2001

An amplification-free hybridization-based DNA assay to detect Streptococcus pneumoniae utilizing the up-converting phosphor technology
journal, July 2003

Zuiderwijk, Michel; Tanke, Hans J.; Sam Niedbala, R.
Clinical Biochemistry, Vol. 36, Issue 5, p. 401-403
https://doi.org/10.1016/S0009-9120(03)00057-2

Similar Records in DOE Patents and OSTI.GOV collections:

Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control

Patent Cary, Robert B.

Highly simplified lateral flow chromatographic nucleic acid sample preparation methods, devices, and integrated systems are provided for the efficient concentration of trace samples and the removal of nucleic acid amplification inhibitors. Methods for capturing and reducing inhibitors of nucleic acid amplification reactions, such as humic acid, using polyvinylpyrrolidone treated elements of the lateral flow device are also provided. Further provided are passive fluid control methods and systems for use in lateral flow assays.
Full Text Available
Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids

Patent Cary, Robert B.

The invention provides miniaturized lateral flow chromatographic and lateral flow chromatographic microarray devices (LFM). The miniaturization of lateral flow nucleic acid detection achieved by the present invention offers reduced reagent use, femtomole sensitivity, excellent linear dynamic range, and rapid detection. Moreover, the small feature sizes of capture oligonucleotides renders the potential information capacity of the platform comparable to more traditional spotted fluorescence microarrays as well as improving sensitivity. The LFM devices exemplified herein enable analytes to be detected within 10 seconds from the time of sample introduction to the LFM device. Sample volumes may be as low as about 10more » « less
Full Text Available
Universal nucleic acids sample preparation method for cells, spores and their mixture

Patent Bavykin, Sergei [Darien, IL]

The present invention relates to a method for extracting nucleic acids from biological samples. More specifically the invention relates to a universal method for extracting nucleic acids from unidentified biological samples. An advantage of the presently invented method is its ability to effectively and efficiently extract nucleic acids from a variety of different cell types including but not limited to prokaryotic or eukaryotic cells and/or recalcitrant organisms (i.e. spores). Unlike prior art methods which are focused on extracting nucleic acids from vegetative cell or spores, the present invention effectively extracts nucleic acids from spores, multiple cell types or mixtures thereofmore » « less
Full Text Available
Repeat sequence chromosome specific nucleic acid probes and methods of preparing and using

Patent Weier, Heinz-Ulrich G [Tracy, CA]; Gray, Joe W [San Francisco, CA]

A primer directed DNA amplification method to isolate efficiently chromosome-specific repeated DNA wherein degenerate oligonucleotide primers are used is disclosed. The probes produced are a heterogeneous mixture that can be used with blocking DNA as a chromosome-specific staining reagent, and/or the elements of the mixture can be screened for high specificity, size and/or high degree of repetition among other parameters. The degenerate primers are sets of primers that vary in sequence but are substantially complementary to highly repeated nucleic acid sequences, preferably clustered within the template DNA, for example, pericentromeric alpha satellite repeat sequences. The template DNA is preferably chromosome-specific.more » « less
Full Text Available
Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid

Patent Koh, Chung-Yan; Light, Yooli Kim; Piccini, Matthew Ernest; ...

Embodiments of the present invention are directed toward devices, systems, and methods for purifying nucleic acids to conduct polymerase chain reaction (PCR) assays. In one example, a method includes generating complexes of silica beads and nucleic acids in a lysis buffer, transporting the complexes through an immiscible fluid to remove interfering compounds from the complexes, further transporting the complexes into a density medium containing components required for PCR where the nucleic acids disassociate from the silica beads, and thermocycling the contents of the density medium to achieve PCR. Signal may be detected from labeling agents in the components required formore » PCR. « less
Full Text Available

Similar Records

Title: Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control

Abstract

Citation Formats

Biochemical analysis of components of the pre-replication complex of Archaeoglobus fulgidus journal, August 2003

One-step immobilization of poly(dT)-modified DNA onto non-modified plastic substrates by UV irradiation for microarrays journal, August 2006

Ligation mediated PCR performed at low denaturation temperatures--PCR melting profiles journal, September 2003

Characterization of a Thermostable UvrD Helicase and Its Participation in Helicase-dependent Amplification journal, June 2005

Shape matters journal, March 2003

Genetic Relationship among Worldwide Strains of Xanthomonas Causing Canker in Citrus Species and Design of New Primers for Their Identification by PCR journal, March 2002

Simultaneous Visual Detection of Multiple Viral Amplicons by Dipstick Assay journal, August 2005

Lab-on-a-chip devices for global health: Past studies and future opportunities journal, January 2007

Signal amplification through nucleotide extension and excision on a dendritic DNA platform journal, April 2000

Accessing Single Nucleotide Polymorphisms in Genomic DNA by Direct Multiplex Polymerase Chain Reaction Amplification on Oligonucleotide Microarrays journal, April 2002

Rapid, Sensitive, and Specific Lateral-Flow Immunochromatographic Device To Measure Anti-Anthrax Protective Antigen Immunoglobulin G in Serum and Whole Blood journal, May 2006

Transmission of the Citrus Variegated Chlorosis Bacterium Xylella fastidiosa with the Sharpshooter Oncometopia nigricans journal, November 2002

Helicase-dependent isothermal DNA amplification journal, August 2004

Genetic Diversity of Pierce's Disease Strains and Other Pathotypes of Xylella fastidiosa journal, February 2001

Direct fluorescence analysis of genetic polymorphisms by hybridization with oligonucleotide arrays on glass supports journal, January 1994

A Universal Nucleic Acid Sequence Biosensor with Nanomolar Detection Limits journal, February 2004

Populations of Xylella fastidiosa in Plants Required for Transmission by an Efficient Vector journal, December 1997

Isothermal reactions for the amplification of oligonucleotides journal, April 2003

Development of immunocapture reverse transcription loop-mediated isothermal amplification for the detection of tomato spotted wilt virus from chrysanthemum journal, October 2004

Biosensors for environmental pollutants and food contaminants journal, October 2003

Capture of Single-Stranded DNA Assisted by Oligonucleotide Modules journal, January 1998

Strand displacement amplification—an isothermal, in vitro DNA amplification technique journal, January 1992

A highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus detection journal, September 1992

Early diagnosis of bubonic plague using F1 antigen capture ELISA assay and rapid immunogold dipstick journal, July 2000

Advances in Polymerase Chain Reaction on Microfluidic Chips journal, June 2005

Helicase structure and mechanism journal, February 2002

Fluorescent Amplified Fragment Length Polymorphism Analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis Isolates journal, February 2004

DnaB from Thermus aquaticus Unwinds Forked Duplex DNA with an Asymmetric Tail Length Dependence journal, March 1999

LNA (locked nucleic acids): synthesis and high-affinity nucleic acid recognition journal, January 1998

Development of a multiplex immunocapture RT-PCR assay for detection and differentiation of tomato and tobacco mosaic tobamoviruses journal, October 1998

Direct isolation of poly(A)+ RNA from 4 M guanidine thiocyanate-lysed cell extracts using locked nucleic acid-oligo(T) capture journal, April 2004

Potent and nontoxic antisense oligonucleotides containing locked nucleic acids journal, May 2000

The total influenza vaccine failure of 1947 revisited: Major intrasubtypic antigenic change can explain failure of vaccine in a post-World War II epidemic journal, July 2002

Detection and Diversity Assessment of Xylella fastidiosa in Field-Collected Plant and Insect Samples by Using 16S rRNA and gyrB Sequences journal, July 2003

Pierce's Disease of Grapevines: Isolation of the Causal Bacterium journal, January 1978

Microarray technology – an intellectual property retrospective journal, September 2003

Multi-analyte single-membrane biosensor for the serotype-specific detection of Dengue virus journal, July 2004

An efficient and economic enhancer mix for PCR journal, September 2006

Design, biochemical, biophysical and biological properties of cooperative antisense oligonucleotides journal, January 1995

Chemiluminescence Detection for Hybridization Assays on the Flow-Thru Chip, a Three-Dimensional Microchannel Biochip journal, December 2001

Multiplex diagnostic platforms for detection of biothreat agents journal, November 2004

High-performance membrane chromatography: Highly efficient separation method for proteins in ion-exchange, hydrophobic interaction and reversed-phase modes journal, September 1993

Identification of A/H5N1 Influenza Viruses Using a Single Gene Diagnostic Microarray journal, January 2007

Using a microfluidic device for 1 l DNA microarray hybridization in 500 s journal, April 2005

Incidence of adamantane resistance among influenza A (H3N2) viruses isolated worldwide from 1994 to 2005: a cause for concern journal, October 2005

Optimization of DNA-tagged dye-encapsulating liposomes for lateral-flow assays based on sandwich hybridization journal, August 2006

Lateral flow microarrays: a novel platform for rapid nucleic acid detection based on miniaturized lateral flow chromatography journal, April 2007

Development of a Novel, Rapid Integrated Cryptosporidium parvum Detection Assay journal, July 2000

Specific PCR detection and identification of Xylella fastidiosa strains causing citrus variegated chlorosis journal, December 1995

Membrane chromatography - an integrative concept in the downstream processing of proteins journal, July 1995

Direct detection of potato leafroll virus in potato tubers by immunocapture and the isothermal nucleic acid amplification method NASBA journal, June 1997

Chromatographic performance of a thin microporous bed of nitrocellulose journal, November 2001

The ligase chain reaction in a PCR world. journal, August 1991

An array of opportunities journal, April 2002

Biosensor for the specific detection of a single viable B. anthracis spore journal, May 2003

Biochemical Characterization of the DNA Helicase Activity of the Escherichia coli RecQ Helicase journal, October 2000

Isolation, Culture, and Pathogenicity of the Bacterium Causing Phony Disease of Peach journal, January 1983

A novel rapid hybridization technique: paper chromatography hybridization assay (PACHA) journal, December 1993

Rapid, direct extraction of DNA from soils for PCR analysis using polyvinylpolypyrrolidone spin columns journal, April 1996

A nested-PCR assay for detection of Xylella fastidiosa in citrus plants and sharpshooter leafhoppers journal, March 2004

Developing rapid, point-of-care, multiplex detection for use in lateral flow devices conference, November 2005

Dependence of the melting temperature of DNA on salt concentration journal, April 1965

Influenza vaccines: present and future journal, July 2002

Current and Developing Technologies for Monitoring Agents of Bioterrorism and Biowarfare journal, October 2005

DNA Detection Using Recombination Proteins journal, June 2006

Microchromatography and microelectrophoresis on nitrocellulose membranes journal, May 1970

Purification and characterization of Thermus thermophilus UvrD journal, February 2003

Fate of Pierce's Disease Strains of Xylella fastidiosa in Common Riparian Plants in California journal, September 1999

Development of a Novel One-Tube Isothermal Reverse Transcription Thermophilic Helicase-Dependent Amplification Platform for Rapid RNA Detection journal, November 2007

Use of laser microdissection greatly improves the recovery of DNA from sperm on microscope slides journal, October 2003

Microfluidic Device for Rapid (<15 min) Automated Microarray Hybridization journal, October 2005

Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase journal, January 1988

The thermodynamic advantage of DNA oligonucleotide 'stacking hybridization' reactions: energetics of a DNA nick journal, February 1997

One step visual detection of PCR products with gold nanoparticles and a nucleic acid lateral flow (NALF) device journal, January 2007

Detection of amantadine-resistant variants among avian influenza viruses isolated in North America and Asia journal, October 2005

Molecular Crowding Regulates the Structural Switch of the DNA G-Quadruplex † journal, December 2002

Detection of Xylella fastidiosa in potential insect vectors by immunomagnetic separation and nested polymerase chain reaction journal, August 1997

Biochemical analysis of components of the pre-replication complex of Archaeoglobus fulgidus
journal, August 2003

One-step immobilization of poly(dT)-modified DNA onto non-modified plastic substrates by UV irradiation for microarrays
journal, August 2006

Ligation mediated PCR performed at low denaturation temperatures--PCR melting profiles
journal, September 2003

Characterization of a Thermostable UvrD Helicase and Its Participation in Helicase-dependent Amplification
journal, June 2005

Shape matters
journal, March 2003

Genetic Relationship among Worldwide Strains of Xanthomonas Causing Canker in Citrus Species and Design of New Primers for Their Identification by PCR
journal, March 2002

Simultaneous Visual Detection of Multiple Viral Amplicons by Dipstick Assay
journal, August 2005

Lab-on-a-chip devices for global health: Past studies and future opportunities
journal, January 2007

Signal amplification through nucleotide extension and excision on a dendritic DNA platform
journal, April 2000

Accessing Single Nucleotide Polymorphisms in Genomic DNA by Direct Multiplex Polymerase Chain Reaction Amplification on Oligonucleotide Microarrays
journal, April 2002

Rapid, Sensitive, and Specific Lateral-Flow Immunochromatographic Device To Measure Anti-Anthrax Protective Antigen Immunoglobulin G in Serum and Whole Blood
journal, May 2006

Transmission of the Citrus Variegated Chlorosis Bacterium Xylella fastidiosa with the Sharpshooter Oncometopia nigricans
journal, November 2002

Helicase-dependent isothermal DNA amplification
journal, August 2004

Genetic Diversity of Pierce's Disease Strains and Other Pathotypes of Xylella fastidiosa
journal, February 2001

Direct fluorescence analysis of genetic polymorphisms by hybridization with oligonucleotide arrays on glass supports
journal, January 1994

A Universal Nucleic Acid Sequence Biosensor with Nanomolar Detection Limits
journal, February 2004

Populations of Xylella fastidiosa in Plants Required for Transmission by an Efficient Vector
journal, December 1997

Isothermal reactions for the amplification of oligonucleotides
journal, April 2003

Development of immunocapture reverse transcription loop-mediated isothermal amplification for the detection of tomato spotted wilt virus from chrysanthemum
journal, October 2004

Biosensors for environmental pollutants and food contaminants
journal, October 2003

Capture of Single-Stranded DNA Assisted by Oligonucleotide Modules
journal, January 1998

Strand displacement amplification—an isothermal, in vitro DNA amplification technique
journal, January 1992

A highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus detection
journal, September 1992

Early diagnosis of bubonic plague using F1 antigen capture ELISA assay and rapid immunogold dipstick
journal, July 2000

Advances in Polymerase Chain Reaction on Microfluidic Chips
journal, June 2005

Helicase structure and mechanism
journal, February 2002

Fluorescent Amplified Fragment Length Polymorphism Analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis Isolates
journal, February 2004

DnaB from Thermus aquaticus Unwinds Forked Duplex DNA with an Asymmetric Tail Length Dependence
journal, March 1999

LNA (locked nucleic acids): synthesis and high-affinity nucleic acid recognition
journal, January 1998

Development of a multiplex immunocapture RT-PCR assay for detection and differentiation of tomato and tobacco mosaic tobamoviruses
journal, October 1998

Direct isolation of poly(A)+ RNA from 4 M guanidine thiocyanate-lysed cell extracts using locked nucleic acid-oligo(T) capture
journal, April 2004

Potent and nontoxic antisense oligonucleotides containing locked nucleic acids
journal, May 2000

The total influenza vaccine failure of 1947 revisited: Major intrasubtypic antigenic change can explain failure of vaccine in a post-World War II epidemic
journal, July 2002

Detection and Diversity Assessment of Xylella fastidiosa in Field-Collected Plant and Insect Samples by Using 16S rRNA and gyrB Sequences
journal, July 2003

Pierce's Disease of Grapevines: Isolation of the Causal Bacterium
journal, January 1978

Microarray technology – an intellectual property retrospective
journal, September 2003

Multi-analyte single-membrane biosensor for the serotype-specific detection of Dengue virus
journal, July 2004

An efficient and economic enhancer mix for PCR
journal, September 2006

Design, biochemical, biophysical and biological properties of cooperative antisense oligonucleotides
journal, January 1995

Chemiluminescence Detection for Hybridization Assays on the Flow-Thru Chip, a Three-Dimensional Microchannel Biochip
journal, December 2001

Multiplex diagnostic platforms for detection of biothreat agents
journal, November 2004

High-performance membrane chromatography: Highly efficient separation method for proteins in ion-exchange, hydrophobic interaction and reversed-phase modes
journal, September 1993

Identification of A/H5N1 Influenza Viruses Using a Single Gene Diagnostic Microarray
journal, January 2007

Using a microfluidic device for 1 l DNA microarray hybridization in 500 s
journal, April 2005

Incidence of adamantane resistance among influenza A (H3N2) viruses isolated worldwide from 1994 to 2005: a cause for concern
journal, October 2005

Optimization of DNA-tagged dye-encapsulating liposomes for lateral-flow assays based on sandwich hybridization
journal, August 2006

Lateral flow microarrays: a novel platform for rapid nucleic acid detection based on miniaturized lateral flow chromatography
journal, April 2007

Development of a Novel, Rapid Integrated Cryptosporidium parvum Detection Assay
journal, July 2000

Specific PCR detection and identification of Xylella fastidiosa strains causing citrus variegated chlorosis
journal, December 1995

Membrane chromatography - an integrative concept in the downstream processing of proteins
journal, July 1995

Direct detection of potato leafroll virus in potato tubers by immunocapture and the isothermal nucleic acid amplification method NASBA
journal, June 1997

Chromatographic performance of a thin microporous bed of nitrocellulose
journal, November 2001

The ligase chain reaction in a PCR world.
journal, August 1991

An array of opportunities
journal, April 2002

Biosensor for the specific detection of a single viable B. anthracis spore
journal, May 2003

Biochemical Characterization of the DNA Helicase Activity of the Escherichia coli RecQ Helicase
journal, October 2000

Isolation, Culture, and Pathogenicity of the Bacterium Causing Phony Disease of Peach
journal, January 1983

A novel rapid hybridization technique: paper chromatography hybridization assay (PACHA)
journal, December 1993

Rapid, direct extraction of DNA from soils for PCR analysis using polyvinylpolypyrrolidone spin columns
journal, April 1996

A nested-PCR assay for detection of Xylella fastidiosa in citrus plants and sharpshooter leafhoppers
journal, March 2004

Developing rapid, point-of-care, multiplex detection for use in lateral flow devices
conference, November 2005

Dependence of the melting temperature of DNA on salt concentration
journal, April 1965

Influenza vaccines: present and future
journal, July 2002

Current and Developing Technologies for Monitoring Agents of Bioterrorism and Biowarfare
journal, October 2005

DNA Detection Using Recombination Proteins
journal, June 2006

Microchromatography and microelectrophoresis on nitrocellulose membranes
journal, May 1970

Purification and characterization of Thermus thermophilus UvrD
journal, February 2003

Fate of Pierce's Disease Strains of Xylella fastidiosa in Common Riparian Plants in California
journal, September 1999

Development of a Novel One-Tube Isothermal Reverse Transcription Thermophilic Helicase-Dependent Amplification Platform for Rapid RNA Detection
journal, November 2007

Use of laser microdissection greatly improves the recovery of DNA from sperm on microscope slides
journal, October 2003

Microfluidic Device for Rapid (<15 min) Automated Microarray Hybridization
journal, October 2005

Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
journal, January 1988

The thermodynamic advantage of DNA oligonucleotide 'stacking hybridization' reactions: energetics of a DNA nick
journal, February 1997

One step visual detection of PCR products with gold nanoparticles and a nucleic acid lateral flow (NALF) device
journal, January 2007

Detection of amantadine-resistant variants among avian influenza viruses isolated in North America and Asia
journal, October 2005

Molecular Crowding Regulates the Structural Switch of the DNA G-Quadruplex ^†
journal, December 2002

Detection of Xylella fastidiosa in potential insect vectors by immunomagnetic separation and nested polymerase chain reaction
journal, August 1997

Optimal conditions for hybridization with oligonucleotides: A study with myc-oncogene DNA probes
journal, April 1988

Identification of the Heme Compound Copurified with Deoxyribonucleic Acid (DNA) from Bloodstains, a Major Inhibitor of Polymerase Chain Reaction (PCR) Amplification
journal, March 1994

Biosensor for Dengue Virus Detection: Sensitive, Rapid, and Serotype Specific
journal, March 2002