Title: Combined hairpin-antisense compositions and methods for modulating expression

Abstract

A nucleotide construct comprising a nucleotide sequence that forms a stem and a loop, wherein the loop comprises a nucleotide sequence that modulates expression of a target, wherein the stem comprises a nucleotide sequence that modulates expression of a target, and wherein the target modulated by the nucleotide sequence in the loop and the target modulated by the nucleotide sequence in the stem may be the same or different. Vectors, methods of regulating target expression, methods of providing a cell, and methods of treating conditions comprising the nucleotide sequence are also disclosed.

Inventors:

Shanklin, John; Nguyen, Tam Huu

Issue Date:

2015-11-24

Research Org.:

Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1226826

Patent Number(s):

9193972

Application Number:

14/452,049

Assignee:

Brookhaven Science Associates, LLC (Upton, NY)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

Show more

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/111 - {General methods applicable to biologically active non-coding nucleic acids}
C12N15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
C12N15/8218 - {Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]}
C12N15/8247 - {involving modified lipid metabolism, e.g. seed oil composition}
C12N2310/11 - Antisense
C12N2310/14 - interfering N.A.
C12N2310/3519 - Fusion with another nucleic acid
C12N2310/53 - partially self-complementary or closed

Show less

DOE Contract Number:  

AC02-98CH10886

Resource Type:

Patent

Resource Relation:

Patent File Date: 2014 Aug 05

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES