Title: Microfluidic devices, systems, and methods for quantifying particles using centrifugal force

Abstract

Embodiments of the present invention are directed toward microfluidic systems, apparatus, and methods for measuring a quantity of cells in a fluid. Examples include a differential white blood cell measurement using a centrifugal microfluidic system. A method may include introducing a fluid sample containing a quantity of cells into a microfluidic channel defined in part by a substrate. The quantity of cells may be transported toward a detection region defined in part by the substrate, wherein the detection region contains a density media, and wherein the density media has a density lower than a density of the cells and higher than a density of the fluid sample. The substrate may be spun such that at least a portion of the quantity of cells are transported through the density media. Signals may be detected from label moieties affixed to the cells.

Inventors:

Schaff, Ulrich Y.; Sommer, Gregory J.; Singh, Anup K.

Issue Date:

Tue Nov 17 00:00:00 EST 2015

Research Org.:

Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1226547

Patent Number(s):

9186668

Application Number:

12/891,956

Assignee:

Sandia Corporation (Albuquerque, NM)

Patent Classifications (CPCs):

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

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B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

B01L2200/028 - Modular arrangements
B01L2200/0647 - Handling flowable solids, e.g. microscopic beads, cells, particles
B01L2200/10 - Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
B01L2200/16 - Reagents, handling or storing thereof
B01L2300/0803 - Disc shape
B01L2300/0816 - Cards, e.g. flat sample carriers usually with flow in two horizontal directions
B01L2300/0867 - Multiple inlets and one sample wells, e.g. mixing, dilution
B01L2400/0409 - centrifugal forces
B01L3/0275 - {Interchangeable or disposable dispensing tips}
B01L3/502 - {with fluid transport, e.g. in multi-compartment structures
B01L3/50215 - {using a float to separate phases}
B01L3/502753 - {characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N33/56972 - {White blood cells}

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DOE Contract Number:  

AC04-94AL85000

Resource Type:

Patent

Resource Relation:

Patent File Date: 2010 Sep 28

Country of Publication:

United States

Language:

English

Subject:

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS