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Title: Strains for the production of flavonoids from glucose

Abstract

The invention relates to the production of flavonoids and flavonoid precursors in cells through recombinant expression of tyrosine ammonia lyase (TAL), 4-coumarate:CoA ligase (4CL), chalcone synthase (CHS), and chalcone isomerase (CHI).

Inventors:
; ;
Issue Date:
Research Org.:
Massachusetts Institute of Technology, Cambridge, MA, (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1225786
Patent Number(s):
9181539
Application Number:
13/101,462
Assignee:
Massachusetts Institute of Technology
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
FC36-07GO17058
Resource Type:
Patent
Resource Relation:
Patent File Date: 2011 May 05
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Stephanopoulos, Gregory, Santos, Christine, and Koffas, Mattheos. Strains for the production of flavonoids from glucose. United States: N. p., 2015. Web.
Stephanopoulos, Gregory, Santos, Christine, & Koffas, Mattheos. Strains for the production of flavonoids from glucose. United States.
Stephanopoulos, Gregory, Santos, Christine, and Koffas, Mattheos. Fri . "Strains for the production of flavonoids from glucose". United States. https://www.osti.gov/servlets/purl/1225786.
@article{osti_1225786,
title = {Strains for the production of flavonoids from glucose},
author = {Stephanopoulos, Gregory and Santos, Christine and Koffas, Mattheos},
abstractNote = {The invention relates to the production of flavonoids and flavonoid precursors in cells through recombinant expression of tyrosine ammonia lyase (TAL), 4-coumarate:CoA ligase (4CL), chalcone synthase (CHS), and chalcone isomerase (CHI).},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {11}
}

Patent:

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Works referenced in this record:

Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids
journal, November 1998


Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design
journal, August 2005


Practical limits of function prediction
journal, January 2000


A "Silent" Polymorphism in the MDR1 Gene Changes Substrate Specificity
journal, January 2007


Polypeptide Release Factors in Prokaryotes and Eukaryotes
journal, January 2002


Human Catechol-O-Methyltransferase Haplotypes Modulate Protein Expression by Altering mRNA Secondary Structure
journal, December 2006


Silent Polymorphisms Speak: How They Affect Pharmacogenomics and the Treatment of Cancer
journal, October 2007


Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different
journal, April 2001


Developments in Directed Evolution for Improving Enzyme Functions
journal, August 2007


Prediction of protein function from protein sequence and structure
journal, January 1999


A Single Mutation Converts a Novel Phosphotyrosine Binding Domain into a Dual-specificity Phosphatase
journal, November 1995


Conversion of a β-Ketoacyl Synthase to a Malonyl Decarboxylase by Replacement of the Active-Site Cysteine with Glutamine
journal, September 1999


Tuning genetic control through promoter engineering
journal, August 2005


Metabolic engineering and applications of flavonoids
journal, April 2001


Biosynthesis and biotechnological production of flavanones: current state and perspectives
journal, May 2009


Increased Malonyl Coenzyme A Biosynthesis by Tuning the Escherichia coli Metabolic Network and Its Application to Flavanone Production
journal, July 2009


Advances in flavonoid research since 1992
journal, November 2000


GeMS: an advanced software package for designing synthetic genes
journal, May 2005


Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli
journal, January 1995


Heterologous production of flavanones in Escherichia coli : potential for combinatorial biosynthesis of flavonoids in bacteria
journal, August 2003


Mutagenesis of the Bacterial RNA Polymerase Alpha Subunit for Improvement of Complex Phenotypes
journal, February 2009


Flavonoid intake and coronary mortality in Finland: a cohort study
journal, February 1996


Total synthesis of long DNA sequences: Synthesis of a contiguous 32-kb polyketide synthase gene cluster
journal, October 2004


Engineering Central Metabolic Pathways for High-Level Flavonoid Production in Escherichia coli
journal, April 2007


Strain Improvement of Recombinant Escherichia coli for Efficient Production of Plant Flavonoids
journal, January 2008


Efficient production of (2S)-flavanones by Escherichia coli containing an artificial biosynthetic gene cluster
journal, March 2005


Engineering and characterization of a superfolder green fluorescent protein
journal, December 2005


Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes
journal, July 2006


Optimization of a heterologous pathway for the production of flavonoids from glucose
journal, July 2011


Discovery of a Substrate Selectivity Switch in Tyrosine Ammonia-Lyase, a Member of the Aromatic Amino Acid Lyase Family
journal, December 2006


Exploring Recombinant Flavonoid Biosynthesis in Metabolically Engineered Escherichia coli
journal, April 2004


Identification, characterization and functional expression of a tyrosine ammonia-lyase and its mutants from the photosynthetic bacterium Rhodobacter sphaeroides
journal, June 2007


Improved production of p-hydroxycinnamic acid from tyrosine using a novel thermostable phenylalanine/tyrosine ammonia lyase enzyme
journal, December 2007