Global to push GA events into
skip to main content

Title: Strains for the production of flavonoids from glucose

The invention relates to the production of flavonoids and flavonoid precursors in cells through recombinant expression of tyrosine ammonia lyase (TAL), 4-coumarate:CoA ligase (4CL), chalcone synthase (CHS), and chalcone isomerase (CHI).
; ;
Issue Date:
OSTI Identifier:
Massachusetts Institute of Technology GFO
Patent Number(s):
Application Number:
Contract Number:
Resource Relation:
Patent File Date: 2011 May 05
Research Org:
Massachusetts Institute of Technology, Cambridge, MA, (United States)
Sponsoring Org:
Country of Publication:
United States

Works referenced in this record:

Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids
journal, November 1998

Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design
journal, August 2005
  • Chica, Roberto A.; Doucet, Nicolas; Pelletier, Joelle N.
  • Current Opinion in Biotechnology, Vol. 16, Issue 4, p. 378-384
  • DOI: 10.1016/j.copbio.2005.06.004

Practical limits of function prediction
journal, January 2000

Polypeptide Release Factors in Prokaryotes and Eukaryotes
journal, January 2002

Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different
journal, April 2001

Developments in Directed Evolution for Improving Enzyme Functions
journal, August 2007
  • Sen, S.; Venkata Dasu, V.; Mandal, B.
  • Applied Biochemistry and Biotechnology, Vol. 143, Issue 3, p. 212-223
  • DOI: 10.1007/s12010-007-8003-4

Prediction of protein function from protein sequence and structure
journal, January 1999
  • Whisstock, James C.; Lesk, Arthur M.
  • Quarterly Reviews of Biophysics, Vol. 36, Issue 3, p. 307-340
  • DOI: 10.1017/S0033583503003901

A Single Mutation Converts a Novel Phosphotyrosine Binding Domain into a Dual-specificity Phosphatase
journal, November 1995
  • Wishart, Matthew J.; Denu, John M.; Williams, John A.
  • Journal of Biological Chemistry, Vol. 270, Issue 45, p. 26782-26785
  • DOI: 10.1074/jbc.270.45.26782

Conversion of a β-Ketoacyl Synthase to a Malonyl Decarboxylase by Replacement of the Active-Site Cysteine with Glutamine
journal, September 1999
  • Witkowski, Andrzej; Joshi, Anil K.; Lindqvist, Ylva
  • Biochemistry, Vol. 38, Issue 36, p. 11643-11650
  • DOI: 10.1021/bi990993h

Tuning genetic control through promoter engineering
journal, August 2005
  • Alper, H.; Fischer, C.; Nevoigt, E.
  • Proceedings of the National Academy of Sciences, Vol. 102, Issue 36, p. 12678-12683
  • DOI: 10.1073/pnas.0504604102

Metabolic engineering and applications of flavonoids
journal, April 2001

Advances in flavonoid research since 1992
journal, November 2000

Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli
journal, January 1995

Total synthesis of long DNA sequences: Synthesis of a contiguous 32-kb polyketide synthase gene cluster
journal, October 2004
  • Kodumal, S. J.; Patel, K. G.; Reid, R.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 44, p. 15573-15578
  • DOI: 10.1073/pnas.0406911101

Engineering and characterization of a superfolder green fluorescent protein
journal, December 2005
  • Pédelacq, Jean-Denis; Cabantous, Stéphanie; Tran, Timothy
  • Nature Biotechnology, Vol. 24, Issue 1, p. 79-88
  • DOI: 10.1038/nbt1172

Optimization of a heterologous pathway for the production of flavonoids from glucose
journal, July 2011
  • Santos, Christine Nicole S.; Koffas, Mattheos; Stephanopoulos, Gregory
  • Metabolic Engineering, Vol. 13, Issue 4, p. 392-400
  • DOI: 10.1016/j.ymben.2011.02.002

Contributions of conserved serine and tyrosine residues to catalysis, ligand binding, and cofactor processing in the active site of tyrosine ammonia lyase
journal, May 2008

Production of p-hydroxycinnamic acid from glucose in Saccharomyces cerevisiae and Escherichia coli by expression of heterologous genes from plants and fungi
journal, March 2007

Discovery of a Substrate Selectivity Switch in Tyrosine Ammonia-Lyase, a Member of the Aromatic Amino Acid Lyase Family
journal, December 2006

Exploring Recombinant Flavonoid Biosynthesis in Metabolically Engineered Escherichia coli
journal, April 2004
  • Watts, Kevin T.; Lee, Pyung Cheon; Schmidt-Dannert, Claudia
  • ChemBioChem, Vol. 5, Issue 4, p. 500-507
  • DOI: 10.1002/cbic.200300783

Improved production of p-hydroxycinnamic acid from tyrosine using a novel thermostable phenylalanine/tyrosine ammonia lyase enzyme
journal, December 2007