Ketol-acid reductoisomerase enzymes and methods of use

Govindarajan, Sridhar; Li, Yougen; Liao, Der-Ing; O'Keefe, Daniel P.; Minshull, Jeremy Stephen; Rothman, Steven Cary; Tobias, Alexander Vincent

Title: Ketol-acid reductoisomerase enzymes and methods of use

Abstract

Provided herein are polypeptides having ketol-aid reductoisomerase activity as well as microbial host cells comprising such polypeptides. Polypeptides provided herein may be used in biosynthetic pathways, including, but not limited to, isobutanol biosynthetic pathways.

Inventors:: Govindarajan, Sridhar; Li, Yougen; Liao, Der-Ing; O'Keefe, Daniel P.; Minshull, Jeremy Stephen; Rothman, Steven Cary; Tobias, Alexander Vincent

Issue Date:: 2015-10-27

Research Org.:: Butamax Advanced Biofuels LLC, Wilmington, DE (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1224448

Patent Number(s):: 9169467

Application Number:: 13/891,963

Assignee:: Butamax Advanced Biofuels LLC (Wilmington, DE)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N9/0006 - {acting on CH-OH groups as donors

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P13/02 - Amides, e.g. chloramphenicol {or polyamides
C12P13/06 - Alanine
C12P13/08 - Lysine
C12P7/16 - Butanols
C12P7/42 - Hydroxy-carboxylic acids
C12P7/44 - Polycarboxylic acids

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y101/01086 - Ketol-acid reductoisomerase

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
Y02E50/10 - Biofuels

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02P - CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
Y02P20/52 - using catalysts, e.g. selective catalysts

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DOE Contract Number:: AR0000006

Resource Type:: Patent

Resource Relation:: Patent File Date: 2013 May 10

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Govindarajan, Sridhar, Li, Yougen, Liao, Der-Ing, O'Keefe, Daniel P., Minshull, Jeremy Stephen, Rothman, Steven Cary, and Tobias, Alexander Vincent. Ketol-acid reductoisomerase enzymes and methods of use.  United States: N. p., 2015. 
        Web.

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                    Govindarajan, Sridhar, Li, Yougen, Liao, Der-Ing, O'Keefe, Daniel P., Minshull, Jeremy Stephen, Rothman, Steven Cary, & Tobias, Alexander Vincent. Ketol-acid reductoisomerase enzymes and methods of use.  United States.

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                    Govindarajan, Sridhar, Li, Yougen, Liao, Der-Ing, O'Keefe, Daniel P., Minshull, Jeremy Stephen, Rothman, Steven Cary, and Tobias, Alexander Vincent. Tue .  
        "Ketol-acid reductoisomerase enzymes and methods of use".  United States.  https://www.osti.gov/servlets/purl/1224448.

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@article{osti_1224448,

  title        = {Ketol-acid reductoisomerase enzymes and methods of use},

  author       = {Govindarajan, Sridhar and Li, Yougen and Liao, Der-Ing and O'Keefe, Daniel P. and Minshull, Jeremy Stephen and Rothman, Steven Cary and Tobias, Alexander Vincent},

  abstractNote = {Provided herein are polypeptides having ketol-aid reductoisomerase activity as well as microbial host cells comprising such polypeptides. Polypeptides provided herein may be used in biosynthetic pathways, including, but not limited to, isobutanol biosynthetic pathways.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2015},

  month        = {10}

}

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Alcohol dehydrogenases (ADH) useful for fermentive production of lower alkyl alcohols patent, July 2014

Increased production of isobutanol in yeast with reduced mitochondrial amino acid biosynthesis patent, July 2014

Yeast with increased butanol tolerance involving cell wall integrity pathway patent, August 2014

Production of isobutanol in yeast mitochondria patent, September 2014

Fermentive production of four carbon alcohols patent, September 2014

Recombinant host cells comprising phosphoketolases patent, October 2014

Fermentive production of four carbon alcohols patent, November 2014

Ketol-acid reductoisomerase using NADH patent, November 2014

Engineering resistance to aliphatic alcohols patent, December 2014

Ketol-acid reductoisomerase using NADH patent, December 2014

Yeast strain for production of four carbon alcohols patent, January 2015

Production of isobutanol by a microorganism with enhanced acetolactate synthase activity patent, February 2015

Ketol-acid reductoisomerase using NADH patent, February 2015

Fermentive production of four carbon alcohols patent, February 2015

Identification and use of bacterial [2Fe-2S] dihydroxy-acid dehydratases patent, February 2015

Enhanced pyruvate to acetolactate conversion in yeast patent, February 2015

Recombinant host cell comprising a diol dehydratase patent, February 2015

Enhanced pyruvate to acetolactate conversion in yeast patent, March 2015

Fermentive production of four carbon alcohols patent, March 2015

Method for producing ethanol using raw starch patent-application, November 2004

Treatment of biomass to obtain fermentable sugars patent-application, February 2007

Fermentative Production Of Four Carbon Alcohols patent-application, April 2007

Fermentive Production Of Four Carbon Alcohols patent-application, July 2008

Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes patent-application, October 2008

Identification and use of Bacterial [2FE-2S] Dihydroxy-acid Dehydratases patent-application, April 2010

Enhanced Iron-sulfur Cluster Formation for Increased Dihydroxy-Acid Dehydratase Activity in Lactic Acid Bacteria patent-application, April 2010

Carbon Pathway Optimized Production Hosts for the Production of Isobutanol patent-application, May 2010

Engineered microorganisms capable of producing target compounds under anaerobic conditions patent-application, June 2021

Cytosolic Isobutanol Pathway Localization for the Production of Isobutanol patent-application, March 2011

Bacterial Strains for Butanol Production patent-application, August 2011

Fermentive Production of Isobutanol Using Highly Effective Ketol-acid Reductoisomerase Enzymes patent-application, October 2011

Engineering Resistance to Aliphatic Alcohols patent-application, March 2012

Fermentive Production of Four Carbon Alcohols patent-application, August 2012

Lignocellulosic Hydrolysates as Feedstocks for Isobutanol Fermentation patent-application, February 2013

Acetate Supplementation of Medium for Butanologens patent-application, September 2013

Host Cells and Methods for Production of Isobutanol patent-application, January 2014

Host Cells and Methods for Production of Isobutanol patent-application, January 2014

Activity of FE-S Cluster Requiring Proteins patent-application, February 2014

Dhad Variants for Butanol Production patent-application, July 2014

Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design journal, August 2005

Developments in Directed Evolution for Improving Enzyme Functions journal, August 2007

Carbon and electron flow in Clostridium butyricum grown in chemostat culture on glycerol and on glucose journal, May 1996

Contribution of VanY D,D-carboxypeptidase to glycopeptide resistance in Enterococcus faecalis by hydrolysis of peptidoglycan precursors. journal, September 1994

Oxalyl hydroxamates as reaction-intermediate analogs for ketol-acid reductoisomerase journal, March 1990

Process for amplifying nucleic acid sequences
patent, July 1987

Process for extractive fermentation
patent, September 1989

Nucleic acid coding for an .alpha.-acetolactate synthase from lactococcus and its applications
patent, July 1997

Method for the production of &rgr;-Hydroxybenzoate in species of pseudomonas and agrobacterium
patent, July 2003

Solvent tolerant microorganisms and methods of isolation
patent, June 2009

Solvent tolerant microorganisms and methods of isolation
patent, February 2010

Fermentive production of four carbon alcohols
patent, December 2010

Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes
patent, March 2011

Fermentive production of four carbon alcohols
patent, August 2011

Solvent tolerant microorganisms
patent, September 2011

Methods of increasing dihydroxy acid dehydratase activity to improve production of fuels, chemicals, and amino acids
patent, December 2011

Ketol-acid reductoisomerase using NADH
patent, March 2012

Fermentive production of four carbon alcohols
patent, May 2012

Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans
patent, May 2012

Production of 2-butanol and 2-butanone employing aminobutanol phosphate phospholyase
patent, June 2012

Ketol-acid reductoisomerase using NADH
patent, July 2012

Recombinant yeast host cell with Fe-S cluster proteins and methods of using thereof
patent, August 2012

Fermentive production of four carbon alcohols
patent, September 2012

Fermentive production of four carbon alcohols
patent, October 2012

Production of four carbon alcohols using improved strain
patent, February 2013

Yeast strain for production of four carbon alcohols
patent, March 2013

Enhanced pyruvate to 2,3-butanediol conversion in lactic acid bacteria
patent, June 2013

Yeast with increased butanol tolerance involving high osmolarity/glycerol response pathway
patent, June 2013

Increased production of isobutanol in yeast with reduced mitochondrial amino acid biosynthesis
patent, June 2013

Strain comprising increased expression of a CFA coding region for butanol production
patent, August 2013

Yeast with increased butanol tolerance involving filamentous growth response
patent, October 2013

Yeast with increased butanol tolerance involving a multidrug efflux pump gene
patent, December 2013

Enhanced dihydroxy-acid dehydratase activity in lactic acid bacteria
patent, January 2014

Expression of hexose kinase in recombinant host cells
patent, January 2014

Strain for butanol production with increased membrane unsaturated trans fatty acids
patent, February 2014

Enhanced pyruvate to acetolactate conversion in yeast
patent, March 2014

Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans
patent, April 2014

Fermentive production of four carbon alcohols
patent, May 2014

Alcohol dehydrogenases (ADH) useful for fermentive production of lower alkyl alcohols
patent, July 2014

Increased production of isobutanol in yeast with reduced mitochondrial amino acid biosynthesis
patent, July 2014

Yeast with increased butanol tolerance involving cell wall integrity pathway
patent, August 2014

Production of isobutanol in yeast mitochondria
patent, September 2014

Fermentive production of four carbon alcohols
patent, September 2014

Recombinant host cells comprising phosphoketolases
patent, October 2014

Fermentive production of four carbon alcohols
patent, November 2014

Ketol-acid reductoisomerase using NADH
patent, November 2014

Engineering resistance to aliphatic alcohols
patent, December 2014

Ketol-acid reductoisomerase using NADH
patent, December 2014

Yeast strain for production of four carbon alcohols
patent, January 2015

Production of isobutanol by a microorganism with enhanced acetolactate synthase activity
patent, February 2015

Ketol-acid reductoisomerase using NADH
patent, February 2015

Fermentive production of four carbon alcohols
patent, February 2015

Identification and use of bacterial [2Fe-2S] dihydroxy-acid dehydratases
patent, February 2015

Enhanced pyruvate to acetolactate conversion in yeast
patent, February 2015

Recombinant host cell comprising a diol dehydratase
patent, February 2015

Enhanced pyruvate to acetolactate conversion in yeast
patent, March 2015

Fermentive production of four carbon alcohols
patent, March 2015

Method for producing ethanol using raw starch
patent-application, November 2004

Treatment of biomass to obtain fermentable sugars
patent-application, February 2007

Fermentative Production Of Four Carbon Alcohols
patent-application, April 2007

Fermentive Production Of Four Carbon Alcohols
patent-application, July 2008

Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes
patent-application, October 2008

Identification and use of Bacterial [2FE-2S] Dihydroxy-acid Dehydratases
patent-application, April 2010

Enhanced Iron-sulfur Cluster Formation for Increased Dihydroxy-Acid Dehydratase Activity in Lactic Acid Bacteria
patent-application, April 2010

Carbon Pathway Optimized Production Hosts for the Production of Isobutanol
patent-application, May 2010

Engineered microorganisms capable of producing target compounds under anaerobic conditions
patent-application, June 2021

Cytosolic Isobutanol Pathway Localization for the Production of Isobutanol
patent-application, March 2011

Bacterial Strains for Butanol Production
patent-application, August 2011

Fermentive Production of Isobutanol Using Highly Effective Ketol-acid Reductoisomerase Enzymes
patent-application, October 2011

Engineering Resistance to Aliphatic Alcohols
patent-application, March 2012

Fermentive Production of Four Carbon Alcohols
patent-application, August 2012

Lignocellulosic Hydrolysates as Feedstocks for Isobutanol Fermentation
patent-application, February 2013

Acetate Supplementation of Medium for Butanologens
patent-application, September 2013

Host Cells and Methods for Production of Isobutanol
patent-application, January 2014

Host Cells and Methods for Production of Isobutanol
patent-application, January 2014

Activity of FE-S Cluster Requiring Proteins
patent-application, February 2014

Dhad Variants for Butanol Production
patent-application, July 2014

Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design
journal, August 2005

Developments in Directed Evolution for Improving Enzyme Functions
journal, August 2007

Carbon and electron flow in Clostridium butyricum grown in chemostat culture on glycerol and on glucose
journal, May 1996

Contribution of VanY D,D-carboxypeptidase to glycopeptide resistance in Enterococcus faecalis by hydrolysis of peptidoglycan precursors.
journal, September 1994

Oxalyl hydroxamates as reaction-intermediate analogs for ketol-acid reductoisomerase
journal, March 1990

The crystal structure of plant acetohydroxy acid isomeroreductase complexed with NADPH, two magnesium ions and a herbicidal transition state analog determined at 1.65Aresolution
journal, June 1997

Targeted Disruption of the kstD Gene Encoding a 3-Ketosteroid Delta 1-Dehydrogenase Isoenzyme of Rhodococcus erythropolis Strain SQ1
journal, May 2000

Mechanism of ketol acid reductoisomerase. Steady-state analysis and metal ion requirement
journal, January 1989