Methods and compositions for rapid thermal cycling

Title: Methods and compositions for rapid thermal cycling

The rapid thermal cycling of a material is targeted. A microfluidic heat exchanger with an internal porous medium is coupled to tanks containing cold fluid and hot fluid. Fluid flows alternately from the cold tank and the hot tank into the porous medium, cooling and heating samples contained in the microfluidic heat exchanger's sample wells. A valve may be coupled to the tanks and a pump, and switching the position of the valve may switch the source and direction of fluid flowing through the porous medium. A controller may control the switching of valve positions based on the temperature of the samples and determined temperature thresholds. A sample tray for containing samples to be thermally cycled may be used in conjunction with the thermal cycling system. A surface or internal electrical heater may aid in heating the samples, or may replace the necessity for the hot tank.

Inventors:: Beer, Neil Reginald; Benett, William J.; Frank, James M.; Deotte, Joshua R.; Spadaccini, Christopher

Issue Date:: 2015-10-27

OSTI Identifier:: 1224445

Assignee:: Lawrence Livermore National Security, LLC (Livermore, CA) LLNL

Patent Number(s):: 9,170,028

Application Number:: 13/267,767

Contract Number:: AC52-07NA27344

Resource Relation:: Patent File Date: 2011 Oct 06

Research Org:: Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

Sponsoring Org:: USDOE

Country of Publication:: United States

Language:: English

Subject:: 42 ENGINEERING

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Other works cited in this record:

On-Chip Single-Copy Real-Time Reverse-Transcription PCR in Isolated Picoliter Droplets
journal, March 2008

Beer, N. Reginald; Wheeler, Elizabeth K.; Lee-Houghton, Lorenna
Analytical Chemistry, Vol. 80, Issue 6, p. 1854-1858
DOI: 10.1021/ac800048k

On-Chip, Real-Time, Single-Copy Polymerase Chain Reaction in Picoliter Droplets
journal, November 2007

Beer, N. Reginald; Hindson, Benjamin J.; Wheeler, Elizabeth K.
Analytical Chemistry, Vol. 79, Issue 22, p. 8471-8475
DOI: 10.1021/ac701809w

Kinetics of the DNA polymerase pyrococcus kodakaraensis
journal, June 2006

Griep, M. A.; Kotera, C. A.; Nelson, R. M.
Chemical Engineering Science, Vol. 61, Issue 12, p. 3885-3892
DOI: 10.1016/j.ces.2005.12.032

Nano/Microfluidics for diagnosis of infectious diseases in developing countries
journal, March 2010

Lee, Won Gu; Kim, Yun-Gon; Chung, Bong Geun
Advanced Drug Delivery Reviews, Vol. 62, Issue 4-5, p. 449-457
DOI: 10.1016/j.addr.2009.11.016

Rapid microfluidic thermal cycler for polymerase chain reaction nucleic acid amplification
journal, May 2008

Mahjoob, Shadi; Vafai, Kambiz; Beer, N. Reginald
International Journal of Heat and Mass Transfer, Vol. 51, Issue 9-10, p. 2109-2122
DOI: 10.1016/j.ijheatmasstransfer.2007.11.014

Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends
journal, June 2007

Zhang, Chunsun; Xing, Da
Nucleic Acids Research, Vol. 35, Issue 13, p. 4223-4237
DOI: 10.1093/nar/gkm389

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