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Title: Methods and compositions for rapid thermal cycling

Abstract

The rapid thermal cycling of a material is targeted. A microfluidic heat exchanger with an internal porous medium is coupled to tanks containing cold fluid and hot fluid. Fluid flows alternately from the cold tank and the hot tank into the porous medium, cooling and heating samples contained in the microfluidic heat exchanger's sample wells. A valve may be coupled to the tanks and a pump, and switching the position of the valve may switch the source and direction of fluid flowing through the porous medium. A controller may control the switching of valve positions based on the temperature of the samples and determined temperature thresholds. A sample tray for containing samples to be thermally cycled may be used in conjunction with the thermal cycling system. A surface or internal electrical heater may aid in heating the samples, or may replace the necessity for the hot tank.

Inventors:
; ; ; ;
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1224445
Patent Number(s):
9170028
Application Number:
13/267,767
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA)
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
C - CHEMISTRY C12 - BIOCHEMISTRY C12M - APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
DOE Contract Number:  
AC52-07NA27344
Resource Type:
Patent
Resource Relation:
Patent File Date: 2011 Oct 06
Country of Publication:
United States
Language:
English
Subject:
42 ENGINEERING

Citation Formats

Beer, Neil Reginald, Benett, William J., Frank, James M., Deotte, Joshua R., and Spadaccini, Christopher. Methods and compositions for rapid thermal cycling. United States: N. p., 2015. Web.
Beer, Neil Reginald, Benett, William J., Frank, James M., Deotte, Joshua R., & Spadaccini, Christopher. Methods and compositions for rapid thermal cycling. United States.
Beer, Neil Reginald, Benett, William J., Frank, James M., Deotte, Joshua R., and Spadaccini, Christopher. Tue . "Methods and compositions for rapid thermal cycling". United States. https://www.osti.gov/servlets/purl/1224445.
@article{osti_1224445,
title = {Methods and compositions for rapid thermal cycling},
author = {Beer, Neil Reginald and Benett, William J. and Frank, James M. and Deotte, Joshua R. and Spadaccini, Christopher},
abstractNote = {The rapid thermal cycling of a material is targeted. A microfluidic heat exchanger with an internal porous medium is coupled to tanks containing cold fluid and hot fluid. Fluid flows alternately from the cold tank and the hot tank into the porous medium, cooling and heating samples contained in the microfluidic heat exchanger's sample wells. A valve may be coupled to the tanks and a pump, and switching the position of the valve may switch the source and direction of fluid flowing through the porous medium. A controller may control the switching of valve positions based on the temperature of the samples and determined temperature thresholds. A sample tray for containing samples to be thermally cycled may be used in conjunction with the thermal cycling system. A surface or internal electrical heater may aid in heating the samples, or may replace the necessity for the hot tank.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {10}
}

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Works referenced in this record:

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