Methods and compositions for rapid thermal cycling
Abstract
The rapid thermal cycling of a material is targeted. A microfluidic heat exchanger with an internal porous medium is coupled to tanks containing cold fluid and hot fluid. Fluid flows alternately from the cold tank and the hot tank into the porous medium, cooling and heating samples contained in the microfluidic heat exchanger's sample wells. A valve may be coupled to the tanks and a pump, and switching the position of the valve may switch the source and direction of fluid flowing through the porous medium. A controller may control the switching of valve positions based on the temperature of the samples and determined temperature thresholds. A sample tray for containing samples to be thermally cycled may be used in conjunction with the thermal cycling system. A surface or internal electrical heater may aid in heating the samples, or may replace the necessity for the hot tank.
- Inventors:
- Issue Date:
- Research Org.:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1224445
- Patent Number(s):
- 9,170,028
- Application Number:
- 13/267,767
- Assignee:
- Lawrence Livermore National Security, LLC (Livermore, CA)
- DOE Contract Number:
- AC52-07NA27344
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2011 Oct 06
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 42 ENGINEERING
Citation Formats
Beer, Neil Reginald, Benett, William J., Frank, James M., Deotte, Joshua R., and Spadaccini, Christopher. Methods and compositions for rapid thermal cycling. United States: N. p., 2015.
Web.
Beer, Neil Reginald, Benett, William J., Frank, James M., Deotte, Joshua R., & Spadaccini, Christopher. Methods and compositions for rapid thermal cycling. United States.
Beer, Neil Reginald, Benett, William J., Frank, James M., Deotte, Joshua R., and Spadaccini, Christopher. Tue .
"Methods and compositions for rapid thermal cycling". United States. https://www.osti.gov/servlets/purl/1224445.
@article{osti_1224445,
title = {Methods and compositions for rapid thermal cycling},
author = {Beer, Neil Reginald and Benett, William J. and Frank, James M. and Deotte, Joshua R. and Spadaccini, Christopher},
abstractNote = {The rapid thermal cycling of a material is targeted. A microfluidic heat exchanger with an internal porous medium is coupled to tanks containing cold fluid and hot fluid. Fluid flows alternately from the cold tank and the hot tank into the porous medium, cooling and heating samples contained in the microfluidic heat exchanger's sample wells. A valve may be coupled to the tanks and a pump, and switching the position of the valve may switch the source and direction of fluid flowing through the porous medium. A controller may control the switching of valve positions based on the temperature of the samples and determined temperature thresholds. A sample tray for containing samples to be thermally cycled may be used in conjunction with the thermal cycling system. A surface or internal electrical heater may aid in heating the samples, or may replace the necessity for the hot tank.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {10}
}
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