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Title: Methods and compositions for rapid thermal cycling

The rapid thermal cycling of a material is targeted. A microfluidic heat exchanger with an internal porous medium is coupled to tanks containing cold fluid and hot fluid. Fluid flows alternately from the cold tank and the hot tank into the porous medium, cooling and heating samples contained in the microfluidic heat exchanger's sample wells. A valve may be coupled to the tanks and a pump, and switching the position of the valve may switch the source and direction of fluid flowing through the porous medium. A controller may control the switching of valve positions based on the temperature of the samples and determined temperature thresholds. A sample tray for containing samples to be thermally cycled may be used in conjunction with the thermal cycling system. A surface or internal electrical heater may aid in heating the samples, or may replace the necessity for the hot tank.
Inventors:
; ; ; ;
Issue Date:
OSTI Identifier:
1224445
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA) LLNL
Patent Number(s):
9,170,028
Application Number:
13/267,767
Contract Number:
AC52-07NA27344
Resource Relation:
Patent File Date: 2011 Oct 06
Research Org:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
42 ENGINEERING

Other works cited in this record:

On-Chip Single-Copy Real-Time Reverse-Transcription PCR in Isolated Picoliter Droplets
journal, March 2008
  • Beer, N. Reginald; Wheeler, Elizabeth K.; Lee-Houghton, Lorenna
  • Analytical Chemistry, Vol. 80, Issue 6, p. 1854-1858
  • DOI: 10.1021/ac800048k

On-Chip, Real-Time, Single-Copy Polymerase Chain Reaction in Picoliter Droplets
journal, November 2007
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  • DOI: 10.1021/ac701809w

Kinetics of the DNA polymerase pyrococcus kodakaraensis
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  • DOI: 10.1016/j.ces.2005.12.032

Nano/Microfluidics for diagnosis of infectious diseases in developing countries
journal, March 2010
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  • Advanced Drug Delivery Reviews, Vol. 62, Issue 4-5, p. 449-457
  • DOI: 10.1016/j.addr.2009.11.016

Rapid microfluidic thermal cycler for polymerase chain reaction nucleic acid amplification
journal, May 2008

Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends
journal, June 2007
  • Zhang, Chunsun; Xing, Da
  • Nucleic Acids Research, Vol. 35, Issue 13, p. 4223-4237
  • DOI: 10.1093/nar/gkm389

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