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Title: Rapid microfluidic thermal cycler for nucleic acid amplification

Abstract

A system for thermal cycling a material to be thermal cycled including a microfluidic heat exchanger; a porous medium in the microfluidic heat exchanger; a microfluidic thermal cycling chamber containing the material to be thermal cycled, the microfluidic thermal cycling chamber operatively connected to the microfluidic heat exchanger; a working fluid at first temperature; a first system for transmitting the working fluid at first temperature to the microfluidic heat exchanger; a working fluid at a second temperature, a second system for transmitting the working fluid at second temperature to the microfluidic heat exchanger; a pump for flowing the working fluid at the first temperature from the first system to the microfluidic heat exchanger and through the porous medium; and flowing the working fluid at the second temperature from the second system to the heat exchanger and through the porous medium.

Inventors:
;
Issue Date:
Research Org.:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1224444
Patent Number(s):
9170060
Application Number:
12/270,348
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA)
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
F - MECHANICAL ENGINEERING F28 - HEAT EXCHANGE IN GENERAL F28F - DETAILS OF HEAT-EXCHANGE AND HEAT-TRANSFER APPARATUS, OF GENERAL APPLICATION
DOE Contract Number:  
AC52-07NA27344
Resource Type:
Patent
Resource Relation:
Patent File Date: 2008 Nov 13
Country of Publication:
United States
Language:
English
Subject:
42 ENGINEERING

Citation Formats

Beer, Neil Reginald, and Vafai, Kambiz. Rapid microfluidic thermal cycler for nucleic acid amplification. United States: N. p., 2015. Web.
Beer, Neil Reginald, & Vafai, Kambiz. Rapid microfluidic thermal cycler for nucleic acid amplification. United States.
Beer, Neil Reginald, and Vafai, Kambiz. Tue . "Rapid microfluidic thermal cycler for nucleic acid amplification". United States. https://www.osti.gov/servlets/purl/1224444.
@article{osti_1224444,
title = {Rapid microfluidic thermal cycler for nucleic acid amplification},
author = {Beer, Neil Reginald and Vafai, Kambiz},
abstractNote = {A system for thermal cycling a material to be thermal cycled including a microfluidic heat exchanger; a porous medium in the microfluidic heat exchanger; a microfluidic thermal cycling chamber containing the material to be thermal cycled, the microfluidic thermal cycling chamber operatively connected to the microfluidic heat exchanger; a working fluid at first temperature; a first system for transmitting the working fluid at first temperature to the microfluidic heat exchanger; a working fluid at a second temperature, a second system for transmitting the working fluid at second temperature to the microfluidic heat exchanger; a pump for flowing the working fluid at the first temperature from the first system to the microfluidic heat exchanger and through the porous medium; and flowing the working fluid at the second temperature from the second system to the heat exchanger and through the porous medium.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {10}
}

Works referenced in this record:

Process for amplifying, detecting, and/or-cloning nucleic acid sequences
patent, July 1987


End-complementary polymerase reaction
patent, November 1998


Method for recombining polynucleotides
patent-application, August 2002


Method for the complete chemical synthesis and assembly of genes and genomes
patent-application, September 2003


Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
patent-application, June 2004


Thermal reaction device and method for using the same
patent-application, November 2005


Thermal reaction device and method for using the same
patent-application, November 2005


Method for carrying out a biochemical protocol in continuous flow in a microreactor
patent-application, December 2005


Pumped fluid cooling system and method
patent-application, March 2006


Thermal cycler for microfluidic array assays
patent-application, May 2006


Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture
patent-application, July 2008


Portable Rapid Microfluidic Thermal Cycler for Extremely Fast Nucleic Acid Amplification
patent-application, September 2009


Isothermal surface production and regulation for high heat flux applications utilizing porous inserts
journal, August 2001


Rapid microfluidic thermal cycler for polymerase chain reaction nucleic acid amplification
journal, May 2008


On-Chip Single-Copy Real-Time Reverse-Transcription PCR in Isolated Picoliter Droplets
journal, March 2008