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Title: Polypeptide having acetyl xylan esterase activity and uses thereof

The invention relates to a polypeptide comprising the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 82% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 82% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.
Inventors:
; ;
Issue Date:
OSTI Identifier:
1223703
Assignee:
DSM IP ASSETS B.V. (Heerlen, NL) GFO
Patent Number(s):
9,163,223
Application Number:
13/805,146
Contract Number:
FC36-08GO18079
Resource Relation:
Patent File Date: 2011 Jun 23
Research Org:
DSM IP ASSETS B.V., Heerlen (Netherlands)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Works referenced in this record:

Characterization of the individual components of the xylanolytic enzyme system of Talaromyces emersonii
journal, January 1994
  • Tuohy, Maria G.; Laffey, Christopher D.; Coughlan, Michael P.
  • Bioresource Technology, Vol. 50, Issue 1, p. 37-42
  • DOI: 10.1016/0960-8524(94)90218-6

Characterization and mode of action of two acetyl xylan esterases from Chrysosporium lucknowense C1 active towards acetylated xylans
journal, August 2011

Biochemical characterization of recombinant acetyl xylan esterase from Aspergillus awamori expressed in Pichia pastoris: Mutational analysis of catalytic residues
journal, May 2005
  • Koseki, Takuya; Miwa, Yozo; Fushinobu, Shinya
  • Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1749, Issue 1, p. 7-13
  • DOI: 10.1016/j.bbapap.2005.01.009