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Title: Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs

Abstract

This invention provides compositions and methods for generating components of protein biosynthetic machinery including orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases. Methods for identifying orthogonal pairs are also provided. These components can be used to incorporate unnatural amino acids into proteins in vivo.

Inventors:
; ; ; ; ; ; ; ; ; ;
Issue Date:
Research Org.:
The Scripps Research Inst., La Jolla, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1223701
Patent Number(s):
9,163,271
Application Number:
13/475,792
Assignee:
The Scripps Research Institute (La Jolla, CA)
DOE Contract Number:  
FG03-00ER45812
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 May 18
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Schultz, Peter G., Wang, Lei, Anderson, John Christopher, Chin, Jason W., Liu, David R., Magliery, Thomas J., Meggers, Eric L., Mehl, Ryan Aaron, Pastrnak, Miro, Santoro, Stephen William, and Zhang, Zhiwen. Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs. United States: N. p., 2015. Web.
Schultz, Peter G., Wang, Lei, Anderson, John Christopher, Chin, Jason W., Liu, David R., Magliery, Thomas J., Meggers, Eric L., Mehl, Ryan Aaron, Pastrnak, Miro, Santoro, Stephen William, & Zhang, Zhiwen. Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs. United States.
Schultz, Peter G., Wang, Lei, Anderson, John Christopher, Chin, Jason W., Liu, David R., Magliery, Thomas J., Meggers, Eric L., Mehl, Ryan Aaron, Pastrnak, Miro, Santoro, Stephen William, and Zhang, Zhiwen. Tue . "Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs". United States. https://www.osti.gov/servlets/purl/1223701.
@article{osti_1223701,
title = {Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs},
author = {Schultz, Peter G. and Wang, Lei and Anderson, John Christopher and Chin, Jason W. and Liu, David R. and Magliery, Thomas J. and Meggers, Eric L. and Mehl, Ryan Aaron and Pastrnak, Miro and Santoro, Stephen William and Zhang, Zhiwen},
abstractNote = {This invention provides compositions and methods for generating components of protein biosynthetic machinery including orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases. Methods for identifying orthogonal pairs are also provided. These components can be used to incorporate unnatural amino acids into proteins in vivo.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {10}
}

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