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Title: Over-expression of NADH-dependent oxidoreductase (fucO) for increasing furfural or 5-hydroxymethylfurfural tolerance

Abstract

The subject invention pertains to the discovery that the NADH-dependent propanediol oxidoreductase (FucO) can reduce furfural. This allows for a new approach to improve furfural tolerance in bacterial and/or yeast cells used to produce desired products. Thus, novel biocatalysts (bacterial, fungal or yeast cells) exhibiting increased tolerance to furfural and 5-hydroxymethylfurfural (5-HMF) are provided as are methods of making and using such biocatalysts for the production of a desired product.

Inventors:
; ; ; ; ;
Issue Date:
Research Org.:
University of Florida Research Foundation, Inc., Gainesville, FL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1223111
Patent Number(s):
9,157,102
Application Number:
14/008,015
Assignee:
University of Florida Research Foundation, Inc. (Gainesville, FL)
DOE Contract Number:  
FG36-08GO88142
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 Mar 29
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Miller, Elliot N., Zhang, Xueli, Yomano, Lorraine P., Wang, Xuan, Shanmugam, Keelnatham T., and Ingram, Lonnie O'Neal. Over-expression of NADH-dependent oxidoreductase (fucO) for increasing furfural or 5-hydroxymethylfurfural tolerance. United States: N. p., 2015. Web.
Miller, Elliot N., Zhang, Xueli, Yomano, Lorraine P., Wang, Xuan, Shanmugam, Keelnatham T., & Ingram, Lonnie O'Neal. Over-expression of NADH-dependent oxidoreductase (fucO) for increasing furfural or 5-hydroxymethylfurfural tolerance. United States.
Miller, Elliot N., Zhang, Xueli, Yomano, Lorraine P., Wang, Xuan, Shanmugam, Keelnatham T., and Ingram, Lonnie O'Neal. Tue . "Over-expression of NADH-dependent oxidoreductase (fucO) for increasing furfural or 5-hydroxymethylfurfural tolerance". United States. https://www.osti.gov/servlets/purl/1223111.
@article{osti_1223111,
title = {Over-expression of NADH-dependent oxidoreductase (fucO) for increasing furfural or 5-hydroxymethylfurfural tolerance},
author = {Miller, Elliot N. and Zhang, Xueli and Yomano, Lorraine P. and Wang, Xuan and Shanmugam, Keelnatham T. and Ingram, Lonnie O'Neal},
abstractNote = {The subject invention pertains to the discovery that the NADH-dependent propanediol oxidoreductase (FucO) can reduce furfural. This allows for a new approach to improve furfural tolerance in bacterial and/or yeast cells used to produce desired products. Thus, novel biocatalysts (bacterial, fungal or yeast cells) exhibiting increased tolerance to furfural and 5-hydroxymethylfurfural (5-HMF) are provided as are methods of making and using such biocatalysts for the production of a desired product.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {10}
}

Patent:

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