Title: Over-expression of NADH-dependent oxidoreductase (fucO) for increasing furfural or 5-hydroxymethylfurfural tolerance

Abstract

The subject invention pertains to the discovery that the NADH-dependent propanediol oxidoreductase (FucO) can reduce furfural. This allows for a new approach to improve furfural tolerance in bacterial and/or yeast cells used to produce desired products. Thus, novel biocatalysts (bacterial, fungal or yeast cells) exhibiting increased tolerance to furfural and 5-hydroxymethylfurfural (5-HMF) are provided as are methods of making and using such biocatalysts for the production of a desired product.

Inventors:

Miller, Elliot N.; Zhang, Xueli; Yomano, Lorraine P.; Wang, Xuan; Shanmugam, Keelnatham T.; Ingram, Lonnie O'Neal

Issue Date:

2015-10-13

Research Org.:

Univ. of Florida, Gainesville, FL (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1223111

Patent Number(s):

9157102

Application Number:

14/008,015

Assignee:

University of Florida Research Foundation, Inc. (Gainesville, FL)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

Show more

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N9/0006 - {acting on CH-OH groups as donors

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P7/06 - Ethanol, i.e. non-beverage
C12P7/065 - {with microorganisms other than yeasts}
C12P7/10 - substrate containing cellulosic material
C12P7/46 - Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
C12P7/56 - Lactic acid

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C12Y101/01202 - 1,3-Propanediol dehydrogenase

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION

Y02E50/10 - Biofuels

Show less

DOE Contract Number:  

FG36-08GO88142

Resource Type:

Patent

Resource Relation:

Patent File Date: 2012 Mar 29

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES