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Title: Over-expression of NADH-dependent oxidoreductase (fucO) for increasing furfural or 5-hydroxymethylfurfural tolerance

The subject invention pertains to the discovery that the NADH-dependent propanediol oxidoreductase (FucO) can reduce furfural. This allows for a new approach to improve furfural tolerance in bacterial and/or yeast cells used to produce desired products. Thus, novel biocatalysts (bacterial, fungal or yeast cells) exhibiting increased tolerance to furfural and 5-hydroxymethylfurfural (5-HMF) are provided as are methods of making and using such biocatalysts for the production of a desired product.
Inventors:
; ; ; ; ;
Issue Date:
OSTI Identifier:
1223111
Assignee:
University of Florida Research Foundation, Inc. (Gainesville, FL) GFO
Patent Number(s):
9,157,102
Application Number:
14/008,015
Contract Number:
FG36-08GO88142
Resource Relation:
Patent File Date: 2012 Mar 29
Research Org:
University of Florida Research Foundation, Inc., Gainesville, FL (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Other works cited in this record:

Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: A review
journal, July 2010

Functional characterization of a stereospecific diol dehydrogenase, FucO, from Escherichia coli: Substrate specificity, pH dependence, kinetic isotope effects and influence of solvent viscosity
journal, September 2010
  • Blikstad, Cecilia; Widersten, Mikael
  • Journal of Molecular Catalysis B: Enzymatic, Vol. 66, Issue 1-2, p. 148-155
  • DOI: 10.1016/j.molcatb.2010.04.010

Optimizing the saccharification of sugar cane bagasse using dilute phosphoric acid followed by fungal cellulases
journal, March 2010

Simplified process for ethanol production from sugarcane bagasse using hydrolysate-resistant Escherichia coli strain MM160
journal, February 2011

Combining metabolic engineering and metabolic evolution to develop nonrecombinant strains of Escherichia coli C that produce succinate and malate
journal, January 2008
  • Jantama, Kaemwich; Haupt, M. J.; Svoronos, Spyros A.
  • Biotechnology and Bioengineering, Vol. 99, Issue 5, p. 1140-1153
  • DOI: 10.1002/bit.21694

Effect of alcohol compounds found in hemicellulose hydrolysate on the growth and fermentation of ethanologenic Escherichia coli
journal, June 2000

Effect of selected aldehydes on the growth and fermentation of ethanologenic Escherichia coli
journal, October 1999

Metabolic evolution of energy-conserving pathways for succinate production in Escherichia coli
journal, November 2009
  • Zhang, X.; Jantama, K.; Moore, J. C.
  • Proceedings of the National Academy of Sciences, Vol. 106, Issue 48, p. 20180-20185
  • DOI: 10.1073/pnas.0905396106

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