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Title: Multifunctional cellulase and hemicellulase

Abstract

A multifunctional polypeptide capable of hydrolyzing cellulosic materials, xylan, and mannan is disclosed. The polypeptide includes the catalytic core (cc) of Clostridium thermocellum Cthe_0797 (CelE), the cellulose-specific carbohydrate-binding module CBM3 of the cellulosome anchoring protein cohesion region (CipA) of Clostridium thermocellum (CBM3a), and a linker region interposed between the catalytic core and the cellulose-specific carbohydrate binding module. Methods of using the multifunctional polypeptide are also disclosed.

Inventors:
; ;
Issue Date:
Research Org.:
Wisconsin Alumni Research Foundation, Madison, WI (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1221980
Patent Number(s):
9145551
Application Number:
14/030,290
Assignee:
Wisconsin Alumni Research Foundation (Madison, WI)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
DOE Contract Number:  
FC02-07ER64494
Resource Type:
Patent
Resource Relation:
Patent File Date: 2013 Sep 18
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Fox, Brian G., Takasuka, Taichi, and Bianchetti, Christopher M. Multifunctional cellulase and hemicellulase. United States: N. p., 2015. Web.
Fox, Brian G., Takasuka, Taichi, & Bianchetti, Christopher M. Multifunctional cellulase and hemicellulase. United States.
Fox, Brian G., Takasuka, Taichi, and Bianchetti, Christopher M. Tue . "Multifunctional cellulase and hemicellulase". United States. https://www.osti.gov/servlets/purl/1221980.
@article{osti_1221980,
title = {Multifunctional cellulase and hemicellulase},
author = {Fox, Brian G. and Takasuka, Taichi and Bianchetti, Christopher M.},
abstractNote = {A multifunctional polypeptide capable of hydrolyzing cellulosic materials, xylan, and mannan is disclosed. The polypeptide includes the catalytic core (cc) of Clostridium thermocellum Cthe_0797 (CelE), the cellulose-specific carbohydrate-binding module CBM3 of the cellulosome anchoring protein cohesion region (CipA) of Clostridium thermocellum (CBM3a), and a linker region interposed between the catalytic core and the cellulose-specific carbohydrate binding module. Methods of using the multifunctional polypeptide are also disclosed.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {9}
}

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Works referenced in this record:

Action of Designer Cellulosomes on Homogeneous Versus Complex Substrates : CONTROLLED INCORPORATION OF THREE DISTINCT ENZYMES INTO A DEFINED TRIFUNCTIONAL SCAFFOLDIN
journal, February 2005


Multiple display of catalytic modules on a protein scaffold: Nano-fabrication of enzyme particles
journal, September 2007


A major new component in the cellulosome of Clostridium thermocellum is a processive endo-β-1,4-glucanase producing cellotetraose
journal, August 2005


Restructuring the Crystalline Cellulose Hydrogen Bond Network Enhances Its Depolymerization Rate
journal, July 2011


Enhanced Bacterial Protein Expression During Auto-Induction Obtained by Alteration of Lac Repressor Dosage and Medium Composition
journal, January 2007


High-throughput Purification and Quality Assurance of Arabidopsis thaliana Proteins for Eukaryotic Structural Genomics
journal, September 2005


A combined approach to improving large-scale production of tobacco etch virus protease
journal, September 2007


Colloid-based multiplexed screening for plant biomass-degrading glycoside hydrolase activities in microbial communities
journal, January 2011


Nanostructure‐Initiator Mass Spectrometry (NIMS) for the Analysis of Enzyme Activities
journal, June 2012


Cellulose-degrading bacteria associated with the invasive woodwasp Sirex noctilio
journal, March 2011


Aerobic deconstruction of cellulosic biomass by an insect-associated Streptomyces
journal, January 2013