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Title: Multifunctional cellulase and hemicellulase

A multifunctional polypeptide capable of hydrolyzing cellulosic materials, xylan, and mannan is disclosed. The polypeptide includes the catalytic core (cc) of Clostridium thermocellum Cthe_0797 (CelE), the cellulose-specific carbohydrate-binding module CBM3 of the cellulosome anchoring protein cohesion region (CipA) of Clostridium thermocellum (CBM3a), and a linker region interposed between the catalytic core and the cellulose-specific carbohydrate binding module. Methods of using the multifunctional polypeptide are also disclosed.
Inventors:
; ;
Issue Date:
OSTI Identifier:
1221980
Assignee:
Wisconsin Alumni Research Foundation (Madison, WI) CHO
Patent Number(s):
9,145,551
Application Number:
14/030,290
Contract Number:
FC02-07ER64494
Resource Relation:
Patent File Date: 2013 Sep 18
Research Org:
Wisconsin Alumni Research Foundation, Madison, WI (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Other works cited in this record:

Multiple display of catalytic modules on a protein scaffold: Nano-fabrication of enzyme particles
journal, September 2007

A combined approach to improving large-scale production of tobacco etch virus protease
journal, September 2007
  • Blommel, Paul G.; Fox, Brian G.
  • Protein Expression and Purification, Vol. 55, Issue 1, p. 53-68
  • DOI: 10.1016/j.pep.2007.04.013

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