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Title: Method for isolating nucleic acids

The current disclosure provides methods and kits for isolating nucleic acid from an environmental sample. The current methods and compositions further provide methods for isolating nucleic acids by reducing adsorption of nucleic acids by charged ions and particles within an environmental sample. The methods of the current disclosure provide methods for isolating nucleic acids by releasing adsorbed nucleic acids from charged particles during the nucleic acid isolation process. The current disclosure facilitates the isolation of nucleic acids of sufficient quality and quantity to enable one of ordinary skill in the art to utilize or analyze the isolated nucleic acids for a wide variety of applications including, sequencing or species population analysis.
Inventors:
;
Issue Date:
OSTI Identifier:
1221979
Assignee:
UT-Battelle, LLC (Oak Ridge, TN) ORNL
Patent Number(s):
9,145,553
Application Number:
13/918,201
Contract Number:
AC05-00OR22725
Resource Relation:
Patent File Date: 2013 Jun 14
Research Org:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Works referenced in this record:

Competitive adsorption, displacement, and transport of organic matter on iron oxide: I. Competitive adsorption
journal, June 1996
  • Gu, Baohua; Mehlhorn, Tonia L.; Liang, Liyuan
  • Geochimica et Cosmochimica Acta, Vol. 60, Issue 11, p. 1943-1950
  • DOI: 10.1016/0016-7037(96)00059-2

Competitive adsorption, displacement, and transport of organic matter on iron oxide: II. Displacement and transport
journal, August 1996
  • Gu, Baohua; Mehlhorn, Tonia L.; Liang, Liyuan
  • Geochimica et Cosmochimica Acta, Vol. 60, Issue 16, p. 2977-2992
  • DOI: 10.1016/0016-7037(96)00157-3