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Title: Method for isolating nucleic acids

Abstract

The current disclosure provides methods and kits for isolating nucleic acid from an environmental sample. The current methods and compositions further provide methods for isolating nucleic acids by reducing adsorption of nucleic acids by charged ions and particles within an environmental sample. The methods of the current disclosure provide methods for isolating nucleic acids by releasing adsorbed nucleic acids from charged particles during the nucleic acid isolation process. The current disclosure facilitates the isolation of nucleic acids of sufficient quality and quantity to enable one of ordinary skill in the art to utilize or analyze the isolated nucleic acids for a wide variety of applications including, sequencing or species population analysis.

Inventors:
;
Issue Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1221979
Patent Number(s):
9145553
Application Number:
13/918,201
Assignee:
UT-Battelle, LLC (Oak Ridge, TN)
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AC05-00OR22725
Resource Type:
Patent
Resource Relation:
Patent File Date: 2013 Jun 14
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Hurt, Jr., Richard Ashley, and Elias, Dwayne A. Method for isolating nucleic acids. United States: N. p., 2015. Web.
Hurt, Jr., Richard Ashley, & Elias, Dwayne A. Method for isolating nucleic acids. United States.
Hurt, Jr., Richard Ashley, and Elias, Dwayne A. Tue . "Method for isolating nucleic acids". United States. https://www.osti.gov/servlets/purl/1221979.
@article{osti_1221979,
title = {Method for isolating nucleic acids},
author = {Hurt, Jr., Richard Ashley and Elias, Dwayne A.},
abstractNote = {The current disclosure provides methods and kits for isolating nucleic acid from an environmental sample. The current methods and compositions further provide methods for isolating nucleic acids by reducing adsorption of nucleic acids by charged ions and particles within an environmental sample. The methods of the current disclosure provide methods for isolating nucleic acids by releasing adsorbed nucleic acids from charged particles during the nucleic acid isolation process. The current disclosure facilitates the isolation of nucleic acids of sufficient quality and quantity to enable one of ordinary skill in the art to utilize or analyze the isolated nucleic acids for a wide variety of applications including, sequencing or species population analysis.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {9}
}

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Works referenced in this record:

High-Sensitivity Stable-Isotope Probing by a Quantitative Terminal Restriction Fragment Length Polymorphism Protocol
journal, October 2011


PCR Amplification-Independent Methods for Detection of Microbial Communities by the High-Density Microarray PhyloChip
journal, July 2011


Competitive adsorption, displacement, and transport of organic matter on iron oxide: I. Competitive adsorption
journal, June 1996


Competitive adsorption, displacement, and transport of organic matter on iron oxide: II. Displacement and transport
journal, August 1996


Simultaneous Recovery of RNA and DNA from Soils and Sediments
journal, October 2001


Bacterial community succession during in situ uranium bioremediation: spatial similarities along controlled flow paths
journal, September 2008


Changes in bacterial community structure correlate with initial operating conditions of a field-scale denitrifying fluidized bed reactor
journal, November 2005