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Title: Microfluidic device for acoustic cell lysis

A microfluidic acoustic-based cell lysing device that can be integrated with on-chip nucleic acid extraction. Using a bulk acoustic wave (BAW) transducer array, acoustic waves can be coupled into microfluidic cartridges resulting in the lysis of cells contained therein by localized acoustic pressure. Cellular materials can then be extracted from the lysed cells. For example, nucleic acids can be extracted from the lysate using silica-based sol-gel filled microchannels, nucleic acid binding magnetic beads, or Nafion-coated electrodes. Integration of cell lysis and nucleic acid extraction on-chip enables a small, portable system that allows for rapid analysis in the field.
Inventors:
; ; ; ;
Issue Date:
OSTI Identifier:
1207283
Assignee:
Sandia Corporation (Albuquerque, NM) SSO
Patent Number(s):
9,096,823
Application Number:
12/872,919
Contract Number:
AC04-94AL85000
Resource Relation:
Patent File Date: 2010 Aug 31
Research Org:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Other works cited in this record:

Microfluidic sonicator for real-time disruption of eukaryotic cells and bacterial spores for DNA analysis
journal, September 2005
  • Marentis, Theodore Cosmo; Kusler, Brenda; Yaralioglu, Goksen G.
  • Ultrasound in Medicine & Biology, Vol. 31, Issue 9, p. 1265-1277
  • DOI: 10.1016/j.ultrasmedbio.2005.05.005

Nucleic acid purification using microfabricated silicon structures
journal, October 2003

Reversible capture of genomic DNA by a Nafion-coated electrode
journal, September 2008
  • Lee, Myoyong; Lee, Jung-Nam; Kim, Jintae
  • Analytical Biochemistry, Vol. 380, Issue 2, p. 335-337
  • DOI: 10.1016/j.ab.2008.06.007

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