Nucleic acid encoding a self-assembling split-fluorescent protein system
Abstract
The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.
- Inventors:
- Issue Date:
- Research Org.:
- Los Alamos National Security, LLC, Los Alamos, NM (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1193372
- Patent Number(s):
- 9081014
- Application Number:
- 14/166,770
- Assignee:
- Los Alamos National Security, LLC (Los Alamos, NM)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FG02-98ER62647
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2014 Jan 28
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Waldo, Geoffrey S., and Cabantous, Stephanie. Nucleic acid encoding a self-assembling split-fluorescent protein system. United States: N. p., 2015.
Web.
Waldo, Geoffrey S., & Cabantous, Stephanie. Nucleic acid encoding a self-assembling split-fluorescent protein system. United States.
Waldo, Geoffrey S., and Cabantous, Stephanie. Tue .
"Nucleic acid encoding a self-assembling split-fluorescent protein system". United States. https://www.osti.gov/servlets/purl/1193372.
@article{osti_1193372,
title = {Nucleic acid encoding a self-assembling split-fluorescent protein system},
author = {Waldo, Geoffrey S. and Cabantous, Stephanie},
abstractNote = {The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {7}
}
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