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Title: Engineering microbes for efficient production of chemicals

Abstract

This present invention relates to production of chemicals from microorganisms that have been genetically engineered and metabolically evolved. Improvements in chemical production have been established, and particular mutations that lead to those improvements have been identified. Specific examples are given in the identification of mutations that occurred during the metabolic evolution of a bacterial strain genetically engineered to produce succinic acid. This present invention also provides a method for evaluating the industrial applicability of mutations that were selected during the metabolic evolution for increased succinic acid production. This present invention further provides microorganisms engineered to have mutations that are selected during metabolic evolution and contribute to improved production of succinic acid, other organic acids and other chemicals of commercial interest.

Inventors:
; ; ; ; ;
Issue Date:
Research Org.:
Myriant Corporation, Quincy, MA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1178677
Patent Number(s):
9,017,976
Application Number:
13/503,999
Assignee:
Myriant Corporation (Quincy, MA)
DOE Contract Number:  
EE0002878
Resource Type:
Patent
Resource Relation:
Patent File Date: 2010 Nov 17
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 54 ENVIRONMENTAL SCIENCES

Citation Formats

Gong, Wei, Dole, Sudhanshu, Grabar, Tammy, Collard, Andrew Christopher, Pero, Janice G, and Yocum, R Rogers. Engineering microbes for efficient production of chemicals. United States: N. p., 2015. Web.
Gong, Wei, Dole, Sudhanshu, Grabar, Tammy, Collard, Andrew Christopher, Pero, Janice G, & Yocum, R Rogers. Engineering microbes for efficient production of chemicals. United States.
Gong, Wei, Dole, Sudhanshu, Grabar, Tammy, Collard, Andrew Christopher, Pero, Janice G, and Yocum, R Rogers. Tue . "Engineering microbes for efficient production of chemicals". United States. https://www.osti.gov/servlets/purl/1178677.
@article{osti_1178677,
title = {Engineering microbes for efficient production of chemicals},
author = {Gong, Wei and Dole, Sudhanshu and Grabar, Tammy and Collard, Andrew Christopher and Pero, Janice G and Yocum, R Rogers},
abstractNote = {This present invention relates to production of chemicals from microorganisms that have been genetically engineered and metabolically evolved. Improvements in chemical production have been established, and particular mutations that lead to those improvements have been identified. Specific examples are given in the identification of mutations that occurred during the metabolic evolution of a bacterial strain genetically engineered to produce succinic acid. This present invention also provides a method for evaluating the industrial applicability of mutations that were selected during the metabolic evolution for increased succinic acid production. This present invention further provides microorganisms engineered to have mutations that are selected during metabolic evolution and contribute to improved production of succinic acid, other organic acids and other chemicals of commercial interest.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {4}
}

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