Hydrogel nanoparticle based immunoassay
Abstract
An immunoassay device incorporating porous polymeric capture nanoparticles within either the sample collection vessel or pre-impregnated into a porous substratum within fluid flow path of the analytical device is presented. This incorporation of capture particles within the immunoassay device improves sensitivity while removing the requirement for pre-processing of samples prior to loading the immunoassay device. A preferred embodiment is coreshell bait containing capture nanoparticles which perform three functions in one step, in solution: a) molecular size sieving, b) target analyte sequestration and concentration, and c) protection from degradation. The polymeric matrix of the capture particles may be made of co-polymeric materials having a structural monomer and an affinity monomer, the affinity monomer having properties that attract the analyte to the capture particle. This device is useful for point of care diagnostic assays for biomedical applications and as field deployable assays for environmental, pathogen and chemical or biological threat identification.
- Inventors:
- Issue Date:
- Research Org.:
- George Mason Research Foundation, Inc., Fairfax, VA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1178255
- Patent Number(s):
- 9012240
- Application Number:
- 13/061,507
- Assignee:
- George Mason Research Foundation, Inc. (Fairfax, VA)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- FC52-04NA25455
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2009 Aug 26
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 47 OTHER INSTRUMENTATION; 59 BASIC BIOLOGICAL SCIENCES; 77 NANOSCIENCE AND NANOTECHNOLOGY
Citation Formats
Liotta, Lance A, Luchini, Alessandra, Petricoin, Emanuel F, and Espina, Virginia. Hydrogel nanoparticle based immunoassay. United States: N. p., 2015.
Web.
Liotta, Lance A, Luchini, Alessandra, Petricoin, Emanuel F, & Espina, Virginia. Hydrogel nanoparticle based immunoassay. United States.
Liotta, Lance A, Luchini, Alessandra, Petricoin, Emanuel F, and Espina, Virginia. Tue .
"Hydrogel nanoparticle based immunoassay". United States. https://www.osti.gov/servlets/purl/1178255.
@article{osti_1178255,
title = {Hydrogel nanoparticle based immunoassay},
author = {Liotta, Lance A and Luchini, Alessandra and Petricoin, Emanuel F and Espina, Virginia},
abstractNote = {An immunoassay device incorporating porous polymeric capture nanoparticles within either the sample collection vessel or pre-impregnated into a porous substratum within fluid flow path of the analytical device is presented. This incorporation of capture particles within the immunoassay device improves sensitivity while removing the requirement for pre-processing of samples prior to loading the immunoassay device. A preferred embodiment is coreshell bait containing capture nanoparticles which perform three functions in one step, in solution: a) molecular size sieving, b) target analyte sequestration and concentration, and c) protection from degradation. The polymeric matrix of the capture particles may be made of co-polymeric materials having a structural monomer and an affinity monomer, the affinity monomer having properties that attract the analyte to the capture particle. This device is useful for point of care diagnostic assays for biomedical applications and as field deployable assays for environmental, pathogen and chemical or biological threat identification.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Apr 21 00:00:00 EDT 2015},
month = {Tue Apr 21 00:00:00 EDT 2015}
}
Works referenced in this record:
Smart Hydrogel Particles: Biomarker Harvesting: One-Step Affinity Purification, Size Exclusion, and Protection against Degradation
journal, January 2008
- Luchini, Alessandra; Geho, David H.; Bishop, Barney
- Nano Letters, Vol. 8, Issue 1
Ligand-Functionalized Core/Shell Microgels with Permselective Shells
journal, December 2004
- Nayak, Satish; Lyon, L. Andrew
- Angewandte Chemie International Edition, Vol. 43, Issue 48, p. 6706-6709
Dry chemistry, lateral flow-reconstituted chromatographic enzyme-driven assays
patent, September 2008
- Piasio, Roger N.; Turner, Nathan
- US Patent Document 7,425,302