Global to push GA events into
skip to main content

Title: Devices, systems, and methods for microscale isoelectric fractionation

Embodiments of the present invention provide devices, systems, and methods for microscale isoelectric fractionation. Analytes in a sample may be isolated according to their isoelectric point within a fractionation microchannel. A microfluidic device according to an embodiment of the invention includes a substrate at least partially defining a fractionation microchannel. The fractionation microchannel has at least one cross-sectional dimension equal to or less than 1 mm. A plurality of membranes of different pHs are disposed in the microchannel. Analytes having an isoelectric point between the pH of the membranes may be collected in a region of the fractionation channel between the first and second membranes through isoelectric fractionation.
Inventors:
; ; ;
Issue Date:
OSTI Identifier:
1178015
Assignee:
Sandia Corporation (Albuquerque, NM) SNL-A
Patent Number(s):
9,005,417
Application Number:
12/243,817
Contract Number:
AC04-94AL85000
Resource Relation:
Patent File Date: 2008 Oct 01
Research Org:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Other works cited in this record:

Multistage Isoelectric Focusing in a Polymeric Microfluidic Chip
journal, December 2005
  • Cui, Huanchun; Horiuchi, Keisuke; Dutta, Prashanta
  • Analytical Chemistry, Vol. 77, Issue 24, p. 7878-7886
  • DOI: 10.1021/ac050781s

Effects of separation length and voltage on isoelectric focusing in a plastic microfluidic device
journal, September 2006

Preparative-scale isoelectric trapping separations using a modified Gradiflow unit
journal, December 2002

The current state of two-dimensional electrophoresis with immobilized pH gradients
journal, April 2000

Recent developments in two-dimensional gel electrophoresis with immobilized pH gradients: Wide pH gradients up to pH 12, longer separation distances and simplified procedures
journal, January 1999

Capillary columns with in situ formed porous monolithic packing for micro high-performance liquid chromatography and capillary electrochromatography
journal, September 1999

Parallel processing in the isoelectric focusing chip
journal, November 2003
  • Zilberstein, Gleb V.; Baskin, Emmanuil M.; Bukshpan, Shmuel
  • ELECTROPHORESIS, Vol. 24, Issue 21, p. 3735-3744
  • DOI: 10.1002/elps.200305663

Parallel isoelectric focusing chip
journal, September 2004
  • Zilberstein, Gleb; Korol, Leonid; Bukshpan, Shmuel
  • PROTEOMICS, Vol. 4, Issue 9, p. 2533-2540
  • DOI: 10.1002/pmic.200300794

Parallel isoelectric focusing II
journal, November 2004
  • Zilberstein, Gleb V.; Baskin, Emmanuil M.; Bukshpan, Shmuel
  • ELECTROPHORESIS, Vol. 25, Issue 21-22, p. 3643-3651
  • DOI: 10.1002/elps.200406117

Detecting Single Stranded DNA with a Solid State Nanopore
journal, October 2005
  • Fologea, Daniel; Gershow, Marc; Ledden, Bradley
  • Nano Letters, Vol. 5, Issue 10, p. 1905-1909
  • DOI: 10.1021/nl051199m

On-Chip Coupling of Isoelectric Focusing and Free Solution Electrophoresis for Multidimensional Separations
journal, March 2003
  • Herr, Amy E.; Molho, Joshua I.; Drouvalakis, Katerina A.
  • Analytical Chemistry, Vol. 75, Issue 5, p. 1180-1187
  • DOI: 10.1021/ac026239a

Microfabrication of a tapered channel for isoelectric focusing with thermally generated pH gradient
journal, October 2002

Bead-based microfluidic immunoassays: The next generation
journal, February 2007

Integration of nanoporous membranes for sample filtration/preconcentration in microchip electrophoresis
journal, December 2006
  • Long, Zhicheng; Liu, Dayu; Ye, Nannan
  • ELECTROPHORESIS, Vol. 27, Issue 24, p. 4927-4934
  • DOI: 10.1002/elps.200600252

Comparison of sample fixation and the use of LDS-751 or anti-CD45 for leukocyte identification in mouse whole blood for flow cytometry
journal, January 2007
  • Maes, Melissa L.; Davidson, Lisa B.; McDonagh, Paul F.
  • Journal of Immunological Methods, Vol. 319, Issue 1-2, p. 79-86
  • DOI: 10.1016/j.jim.2006.10.016

Functional integration of DNA purification and concentration into a real time micro-PCR chip
journal, January 2011
  • Min, Junhong; Kim, Joon-Ho; Lee, Youngsun
  • Lab on a Chip, Vol. 11, Issue 2, p. 259-265
  • DOI: 10.1039/C0LC00320D

Plasma DNA concentration as a predictor of mortality and sepsis in critically ill patients
journal, April 2006
  • Rhodes, Andrew; Wort, Stephen J.; Thomas, Helen
  • Critical Care, Vol. 10, Issue 2, Article No. R60
  • DOI: 10.1186/cc4894

A B Cell-Based Sensor for Rapid Identification of Pathogens
journal, July 2003
  • Rider, Todd H.; Petrovick, Martha S.; Nargi, Frances E.
  • Science, Vol. 301, Issue 5630, p. 213-215
  • DOI: 10.1126/science.1084920

Read-out concepts for multiplexed bead-based fluorescence immunoassays on centrifugal microfluidic platforms
journal, February 2006
  • Riegger, L.; Grumann, M.; Nann, T.
  • Sensors and Actuators A: Physical, Vol. 126, Issue 2, p. 455-462
  • DOI: 10.1016/j.sna.2005.11.006

The Alpher, Bethe and Gamow of IEF, the alpha-Centaury of electrokinetic methodologies. Part II: Immobilized pH gradients
journal, February 2007

The Alpher, Bethe, Gamow of isoelectric focusing, the alpha-Centaury of electrokinetic methodologies. Part I
journal, March 2006

Whole Blood Immunoassay Based on Centrifugal Bead Sedimentation
journal, March 2011

Miniaturized capillary isoelectric focusing in plastic microfluidic devices
journal, October 2002

Rapid isoelectric trapping in a micropreparative-scale multicompartment electrolyzer
journal, June 2007
  • Lim, Peniel; North, Robert; Vigh, Gyula
  • ELECTROPHORESIS, Vol. 28, Issue 12, p. 1851-1859
  • DOI: 10.1002/elps.200600846

On-Chip Isoelectric Focusing Using Photopolymerized Immobilized pH Gradients
journal, May 2008
  • Sommer, Greg J.; Singh, Anup K.; Hatch, Anson V.
  • Analytical Chemistry, Vol. 80, Issue 9, p. 3327-3333
  • DOI: 10.1021/ac702523g

A new biodosimetric method: branched DNA-based quantitative detection of B1 DNA in mouse plasma
journal, August 2010
  • Zhang, L.; Zhang, M.; Yang, S.
  • The British Journal of Radiology, Vol. 83, Issue 992, p. 694-701
  • DOI: 10.1259/bjr/49886569

Circulating DNA: a new diagnostic gold mine?
journal, October 2002
  • Ziegler, Annemarie; Zangemeister-Wittke, Uwe; Stahel, Rolf A.
  • Cancer Treatment Reviews, Vol. 28, Issue 5, p. 255-271
  • DOI: 10.1016/S0305-7372(02)00077-4

Similar records in DOepatents and OSTI.GOV collections: