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Title: EGVII endoglucanase and nucleic acids encoding the same

The present invention provides a novel endoglucanase nucleic acid sequence, designated egl7, and the corresponding EGVII amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVII, recombinant EGVII proteins and methods for producing the same.
Inventors:
; ; ;
Issue Date:
OSTI Identifier:
1178008
Assignee:
Danisco US Inc. (Palo Alto, CA) NREL
Patent Number(s):
9,005,948
Application Number:
14/159,044
Contract Number:
AC36-99GO10337
Resource Relation:
Patent File Date: 2014 Jan 20
Research Org:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Works referenced in this record:

Basic local alignment search tool
journal, October 1990
  • Altschul, Stephen F.; Gish, Warren; Miller, Webb
  • Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410
  • DOI: 10.1016/S0022-2836(05)80360-2

Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
journal, September 1997
  • Altschul, Stephen F.; Madden, Thomas L.; Schäffer, Alejandro A.
  • Nucleic Acids Research, Vol. 25, Issue 17, p. 3389-3402
  • DOI: 10.1093/nar/25.17.3389

ACEII, a Novel Transcriptional Activator Involved in Regulation of Cellulase and Xylanase Genes of Trichoderma reesei
journal, April 2001
  • Aro, Nina; Saloheimo, Anu; Ilmén, Marja
  • Journal of Biological Chemistry, Vol. 276, Issue 26, p. 24309-24314
  • DOI: 10.1074/jbc.M003624200

Current Protocols in Molecular Biology
book, May 2001

Cloning and Amplification of the Gene Encoding an Extracellular β-Glucosidase from Trichoderma reesei: Evidence for Improved Rates of Saccharification of Cellulosic Substrates
journal, June 1991
  • Barnett, Christopher C.; Berka, Randy M.; Fowler, Tim
  • Nature Biotechnology, Vol. 9, Issue 6, p. 562-567
  • DOI: 10.1038/nbt0691-562

Fractionation of cellulase and β-glucosidase in a Trichoderma reesei culture liquid by use of two-phase partitioning
journal, November 1999
  • Brumbauer, Anikó; Johansson, Göte; Réczey, Kati
  • Bioseparation, Vol. 7, Issue 6, p. 287-295
  • DOI: 10.1023/A:1008153418019

Purification and characterization of two extracellular β-glucosidases from Trichoderma reesei
journal, May 1992
  • Chen, Huizhong; Hayn, Marianne; Esterbauer, Hermann
  • Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol. 1121, Issue 1-2, p. 54-60
  • DOI: 10.1016/0167-4838(92)90336-C

Genetically engineered peptide fusions for improved protein partitioning in aqueous two-phase systems
journal, March 2001

Analytical separation of Trichoderma reesei cellulases by ion-exchange fast protein liquid chromatography
journal, January 1987

Purification and characterization of a β-glucosidase from solid-state cultures of Humicola grisea var. thermoidea
journal, January 1996
  • Filho, Edivaldo Ximenes Ferreira
  • Canadian Journal of Microbiology, Vol. 42, Issue 1, p. 1-5
  • DOI: 10.1139/m96-001

Characterization of cellulases by HPLC separation
journal, January 1983
  • Fliess, A.; Schügerl, K.
  • European Journal of Applied Microbiology and Biotechnology, Vol. 17, Issue 5, p. 314-318
  • DOI: 10.1007/BF00508028

Characteristics of fungal cellulases
journal, January 1991

Cloning, sequencing and overexpression of a Rhodothermus marinus gene encoding a thermostable cellulase of glycosyl hydrolase family 12
journal, March 1998
  • Halldórsdóttir, S.; Thórólfsdót, E. T.; Spilliaert, R.
  • Applied Microbiology and Biotechnology, Vol. 49, Issue 3, p. 277-284
  • DOI: 10.1007/s002530051169

Purification and properties of an extracellular β-glucosidase from Lenzites trabea
journal, January 1978
  • Herr, D.; Baumer, F.; Dellweg, H.
  • European Journal of Applied Microbiology and Biotechnology, Vol. 5, Issue 1, p. 29-36
  • DOI: 10.1007/BF00515684

Antibodies specific for the human retinoblastoma protein identify a family of related polypeptides.
journal, November 1991
  • Hu, Q. J.; Bautista, C.; Edwards, G. M.
  • Molecular and Cellular Biology, Vol. 11, Issue 11, p. 5792-5799
  • DOI: 10.1128/MCB.11.11.5792

Production of fully human antibodies by transgenic mice
journal, January 1995

The First Structure of a Glycoside Hydrolase Family 61 Member, Cel61B from Hypocrea jecorina, at 1.6 Å Resolution
journal, October 2008
  • Karkehabadi, Saeid; Hansson, Henrik; Kim, Steve
  • Journal of Molecular Biology, Vol. 383, Issue 1, p. 144-154
  • DOI: 10.1016/j.jmb.2008.08.016

Cloning and sequencing of the cDNA encoding β-glucosidase 1 from Aspergillus aculeatus
journal, September 1996

Cellulase families and their genes
journal, September 1987
  • Knowles, Jonathan; Lehtovaara, Päivi; Teeri, Tuula
  • Trends in Biotechnology, Vol. 5, Issue 9, p. 255-261
  • DOI: 10.1016/0167-7799(87)90102-8

Continuous cultures of fused cells secreting antibody of predefined specificity
journal, August 1975
  • Köhler, G.; Milstein, C.
  • Nature, Vol. 256, Issue 5517, p. 495-497
  • DOI: 10.1038/256495a0

Simultaneous saccharification and fermentation of lignocellulosic wastes to ethanol using a thermotolerant yeast
journal, April 2001

Directed immobilization of recombinant staphylococci on cotton fibers by functional display of a fungal cellulose-binding domain
journal, February 2001

The roles and function of cellulose-binding domains
journal, September 1997

Kinetics of cellulose saccharification by Trichoderma reesei cellulases
journal, January 2001

Efficient secretion of two fungal cellobiohydrolases by Saccharomyces cerevisiae
journal, March 1988

Homology between cellulase genes of Trichoderma reesei: complete nucleotide sequence of the endoglucanase I gene
journal, January 1986

Synthesis and secretion of wheat α-amylase in Saccharomyces cerevisiae
journal, January 1987

CelS: a novel endoglucanase identified from Erwinia carotovora subsp. carotovora
journal, May 1990

Cloning and sequencing of cellulase cDNA from Aspergillus kawachii and its expression in Saccharomyces cerevisiae
journal, April 1995
  • Sakamoto, S.; Tamura, G.; Ito, K.
  • Current Genetics, Vol. 27, Issue 5, p. 435-439
  • DOI: 10.1007/BF00311212

A novel, small endoglucanase gene, egl5, from Trichoderma reesei isolated by expression in yeast
journal, July 1994

EGIII, a new endoglucanase from Trichoderma reesei: the characterization of both gene and enzyme
journal, March 1988

cDNA Cloning of a Trichoderma reesei Cellulase and Demonstration of Endoglucanase Activity by Expression in Yeast
journal, October 1997
  • Saloheimo, Markku; Nakari-SetaLa, Tiina; Tenkanen, Maija
  • European Journal of Biochemistry, Vol. 249, Issue 2, p. 584-591
  • DOI: 10.1111/j.1432-1033.1997.00584.x

[79] Purification of all glycolytic enzymes from one muscle extract
journal, January 1982

Molecular Cloning of Exo–Cellobiohydrolase I Derived from Trichoderma Reesei Strain L27
journal, October 1983
  • Shoemaker, S.; Schweickart, V.; Ladner, M.
  • Nature Biotechnology, Vol. 1, Issue 8, p. 691-696
  • DOI: 10.1038/nbt1083-691

Cloning and Sequencing of a Rhodothermus marinus Gene, bglA, Coding for a Thermostable beta-Glucanase and its Expression in Escherichia coli
journal, September 1994
  • Spilliaert, Remi; Hreggvidsson, Gudmundur Oli; Kristjansson, Jakob Kristjan
  • European Journal of Biochemistry, Vol. 224, Issue 3, p. 923-930
  • DOI: 10.1111/j.1432-1033.1994.00923.x

A New Model For Enzymatic Hydrolysis of Cellulose Based on the Two-Domain Structure of Cellobiohydrolase I
journal, March 1991
  • Ståhlberg, Jerry; Johansson, Gunnar; Pettersson, Göran
  • Nature Biotechnology, Vol. 9, Issue 3, p. 286-290
  • DOI: 10.1038/nbt0391-286

Trichoderma reesei cellulases and their core domains in the hydrolysis and modification of chemical pulp
journal, June 2000

Separation of endo- and exo-type cellulases using a new affinity chromatography method
journal, April 1984
  • van Tilbeurgh, Herman; Bhikhabhai, Ramagauri; Göran Pettersson, L.
  • FEBS Letters, Vol. 169, Issue 2, p. 215-218
  • DOI: 10.1016/0014-5793(84)80321-X

Homologous domains in Trichoderma reesei cellulolytic enzymes: Gene sequence and expression of cellobiohydrolase II
journal, January 1987

Organization of a gene cluster expressed specifically in the asexual spores of A. nidulans
journal, October 1981

Studies on the chromatographic fractionation of Trichoderma reesei cellulases by hydrophobic interaction
journal, December 1999

Studies of the cellulolytic system of Trichoderma reesei QM 9414. Analysis of domain function in two cellobiohydrolases by limited proteolysis
journal, January 1988

Codon optimization of xylanase gene xynB from the thermophilic bacterium Dictyoglomus thermophilum for expression in the filamentous fungus Trichoderma reesei
journal, September 2000

Engineering yeast for efficient cellulose degradation
journal, January 1998

Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites
journal, January 1985

Methods for measuring cellulase activities
journal, January 1988

Properties of cellulolytic enzyme systems
journal, April 1985
  • Wood, Thomas M.
  • Biochemical Society Transactions, Vol. 13, Issue 2, p. 407-410
  • DOI: 10.1042/bst0130407