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Title: Buoyant triacylglycerol-filled green algae and methods therefor

Cultures of Chlamydomonas are disclosed comprising greater than 340 mg/l triacylglycerols (TAG). The cultures can include buoyant Chlamydomonas. Methods of forming the cultures are also disclosed. In some embodiments, these methods comprise providing Chlamydomonas growing in log phase in a first culture medium comprising a nitrogen source and acetate, replacing the first culture medium with a second medium comprising acetate but no nitrogen source, and subsequently supplementing the second medium with additional acetate. In some embodiments, a culture can comprise at least 1,300 mg/l triacyglycerols. In some embodiments, cultures can be used to produce a biofuel such as biodiesel.
Inventors:
;
Issue Date:
OSTI Identifier:
1178007
Assignee:
Washington University (Saint Louis, MO) DOESC
Patent Number(s):
9,005,955
Application Number:
13/662,549
Resource Relation:
Patent File Date: 2012 Oct 29
Research Org:
Washington University, Saint Louis, MO (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS

Other works cited in this record:

A chloroplast pathway for the de novo biosynthesis of triacylglycerol in Chlamydomonas reinhardtii
journal, May 2011

Prospects for molecular farming in the green alga Chlamydomonas reinhardtii
journal, April 2004
  • Franklin, Scott E.; Mayfield, Stephen P.
  • Current Opinion in Plant Biology, Vol. 7, Issue 2, p. 159-165
  • DOI: 10.1016/j.pbi.2004.01.012

Oil extraction from microalgae for biodiesel production
journal, January 2011

Chlamydomonas starchless mutant defective in ADP-glucose pyrophosphorylase hyper-accumulates triacylglycerol
journal, July 2010

Inhibition of starch synthesis results in overproduction of lipids in Chlamydomonas reinhardtii
journal, May 2010
  • Li, Yantao; Han, Danxiang; Hu, Guangrong
  • Biotechnology and Bioengineering, Vol. 107, Issue 2, p. 258-268
  • DOI: 10.1002/bit.22807

The Polyphosphate Bodies of Chlamydomonas reinhardtii Possess a Proton-pumping Pyrophosphatase and Are Similar to Acidocalcisomes
journal, September 2001
  • Ruiz, Felix A.; Marchesini, Norma; Seufferheld, Manfredo
  • Journal of Biological Chemistry, Vol. 276, Issue 49, p. 46196-46203
  • DOI: 10.1074/jbc.M105268200

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