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Title: Buoyant triacylglycerol-filled green algae and methods therefor

Abstract

Cultures of Chlamydomonas are disclosed comprising greater than 340 mg/l triacylglycerols (TAG). The cultures can include buoyant Chlamydomonas. Methods of forming the cultures are also disclosed. In some embodiments, these methods comprise providing Chlamydomonas growing in log phase in a first culture medium comprising a nitrogen source and acetate, replacing the first culture medium with a second medium comprising acetate but no nitrogen source, and subsequently supplementing the second medium with additional acetate. In some embodiments, a culture can comprise at least 1,300 mg/l triacyglycerols. In some embodiments, cultures can be used to produce a biofuel such as biodiesel.

Inventors:
;
Issue Date:
Research Org.:
Washington University, Saint Louis, MO (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1178007
Patent Number(s):
9,005,955
Application Number:
13/662,549
Assignee:
Washington University (Saint Louis, MO)
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 Oct 29
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS

Citation Formats

Goodenough, Ursula, and Goodson, Carrie. Buoyant triacylglycerol-filled green algae and methods therefor. United States: N. p., 2015. Web.
Goodenough, Ursula, & Goodson, Carrie. Buoyant triacylglycerol-filled green algae and methods therefor. United States.
Goodenough, Ursula, and Goodson, Carrie. Tue . "Buoyant triacylglycerol-filled green algae and methods therefor". United States. https://www.osti.gov/servlets/purl/1178007.
@article{osti_1178007,
title = {Buoyant triacylglycerol-filled green algae and methods therefor},
author = {Goodenough, Ursula and Goodson, Carrie},
abstractNote = {Cultures of Chlamydomonas are disclosed comprising greater than 340 mg/l triacylglycerols (TAG). The cultures can include buoyant Chlamydomonas. Methods of forming the cultures are also disclosed. In some embodiments, these methods comprise providing Chlamydomonas growing in log phase in a first culture medium comprising a nitrogen source and acetate, replacing the first culture medium with a second medium comprising acetate but no nitrogen source, and subsequently supplementing the second medium with additional acetate. In some embodiments, a culture can comprise at least 1,300 mg/l triacyglycerols. In some embodiments, cultures can be used to produce a biofuel such as biodiesel.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {4}
}

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Works referenced in this record:

Evolution of acidocalcisomes and their role in polyphosphate storage and osmoregulation in eukaryotic microbes
journal, March 2010

  • Docampo, Roberto; Ulrich, Paul; Moreno, Silvia N. J.
  • Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 365, Issue 1541
  • DOI: 10.1098/rstb.2009.0179

A chloroplast pathway for the de novo biosynthesis of triacylglycerol in Chlamydomonas reinhardtii
journal, May 2011


Prospects for molecular farming in the green alga Chlamydomonas reinhardtii
journal, April 2004

  • Franklin, Scott E.; Mayfield, Stephen P.
  • Current Opinion in Plant Biology, Vol. 7, Issue 2, p. 159-165
  • DOI: 10.1016/j.pbi.2004.01.012

Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardi.
journal, December 1965

  • Gorman, D. S.; Levine, R. P.
  • Proceedings of the National Academy of Sciences, Vol. 54, Issue 6
  • DOI: 10.1073/pnas.54.6.1665

Oil extraction from microalgae for biodiesel production
journal, January 2011


Chlamydomonas starchless mutant defective in ADP-glucose pyrophosphorylase hyper-accumulates triacylglycerol
journal, July 2010


Inhibition of starch synthesis results in overproduction of lipids in Chlamydomonas reinhardtii
journal, May 2010

  • Li, Yantao; Han, Danxiang; Hu, Guangrong
  • Biotechnology and Bioengineering, Vol. 107, Issue 2, p. 258-268
  • DOI: 10.1002/bit.22807

Gametic differentiation in Chlamydomonas reinhardtii. I. Production of gametes and their fine structure
journal, November 1975


Changes in Transcript Abundance in Chlamydomonas reinhardtii following Nitrogen Deprivation Predict Diversion of Metabolism
journal, October 2010

  • Miller, Rachel; Wu, Guangxi; Deshpande, Rahul R.
  • Plant Physiology, Vol. 154, Issue 4
  • DOI: 10.1104/pp.110.165159

RNA Interference Silencing of a Major Lipid Droplet Protein Affects Lipid Droplet Size in Chlamydomonas reinhardtii
journal, November 2009

  • Moellering, Eric R.; Benning, Christoph
  • Eukaryotic Cell, Vol. 9, Issue 1
  • DOI: 10.1128/EC.00203-09

The Polyphosphate Bodies of Chlamydomonas reinhardtii Possess a Proton-pumping Pyrophosphatase and Are Similar to Acidocalcisomes
journal, September 2001

  • Ruiz, Felix A.; Marchesini, Norma; Seufferheld, Manfredo
  • Journal of Biological Chemistry, Vol. 276, Issue 49, p. 46196-46203
  • DOI: 10.1074/jbc.M105268200

Suppression of Soybean Oleosin Produces Micro-Oil Bodies that Aggregate into Oil Body/ER Complexes
journal, November 2008

  • Schmidt, Monica A.; Herman, Eliot M.
  • Molecular Plant, Vol. 1, Issue 6
  • DOI: 10.1093/mp/ssn049

<i>Entophlyctis apiculata</i>, a chytrid parasite of <i>Chlamydomonas</i> sp. (Chlorophyceae)
journal, January 2001

  • Shin, Woongghi; Boo, Sung Min; Longcore, Joyce E.
  • Canadian Journal of Botany, Vol. 79, Issue 9
  • DOI: 10.1139/cjb-79-9-1083

Algal Lipid Bodies: Stress Induction, Purification, and Biochemical Characterization in Wild-Type and Starchless Chlamydomonas reinhardtii
journal, October 2009

  • Wang, Zi Teng; Ullrich, Nico; Joo, Sunjoo
  • Eukaryotic Cell, Vol. 8, Issue 12
  • DOI: 10.1128/EC.00272-09

Growth and reproduction of Daphnia galeata in response to changes in fatty acids, phosphorus, and nitrogen in Chlamydomonas reinhardtii
journal, November 1997


Starchless Mutants of Chlamydomonas reinhardtii Lack the Small Subunit of a Heterotetrameric ADP-Glucose Pyrophosphorylase
journal, February 2001


Increased Lipid Accumulation in the Chlamydomonas reinhardtii sta7-10 Starchless Isoamylase Mutant and Increased Carbohydrate Synthesis in Complemented Strains
journal, June 2010

  • Work, Victoria H.; Radakovits, Randor; Jinkerson, Robert E.
  • Eukaryotic Cell, Vol. 9, Issue 8
  • DOI: 10.1128/EC.00075-10