skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Buoyant triacylglycerol-filled green algae and methods therefor

Abstract

Cultures of Chlamydomonas are disclosed comprising greater than 340 mg/l triacylglycerols (TAG). The cultures can include buoyant Chlamydomonas. Methods of forming the cultures are also disclosed. In some embodiments, these methods comprise providing Chlamydomonas growing in log phase in a first culture medium comprising a nitrogen source and acetate, replacing the first culture medium with a second medium comprising acetate but no nitrogen source, and subsequently supplementing the second medium with additional acetate. In some embodiments, a culture can comprise at least 1,300 mg/l triacyglycerols. In some embodiments, cultures can be used to produce a biofuel such as biodiesel.

Inventors:
;
Issue Date:
Research Org.:
Washington University, Saint Louis, MO (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1178007
Patent Number(s):
9005955
Application Number:
13/662,549
Assignee:
Washington University (Saint Louis, MO)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 Oct 29
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS

Citation Formats

Goodenough, Ursula, and Goodson, Carrie. Buoyant triacylglycerol-filled green algae and methods therefor. United States: N. p., 2015. Web.
Goodenough, Ursula, & Goodson, Carrie. Buoyant triacylglycerol-filled green algae and methods therefor. United States.
Goodenough, Ursula, and Goodson, Carrie. Tue . "Buoyant triacylglycerol-filled green algae and methods therefor". United States. https://www.osti.gov/servlets/purl/1178007.
@article{osti_1178007,
title = {Buoyant triacylglycerol-filled green algae and methods therefor},
author = {Goodenough, Ursula and Goodson, Carrie},
abstractNote = {Cultures of Chlamydomonas are disclosed comprising greater than 340 mg/l triacylglycerols (TAG). The cultures can include buoyant Chlamydomonas. Methods of forming the cultures are also disclosed. In some embodiments, these methods comprise providing Chlamydomonas growing in log phase in a first culture medium comprising a nitrogen source and acetate, replacing the first culture medium with a second medium comprising acetate but no nitrogen source, and subsequently supplementing the second medium with additional acetate. In some embodiments, a culture can comprise at least 1,300 mg/l triacyglycerols. In some embodiments, cultures can be used to produce a biofuel such as biodiesel.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {4}
}

Patent:

Save / Share:

Works referenced in this record:

Evolution of acidocalcisomes and their role in polyphosphate storage and osmoregulation in eukaryotic microbes
journal, March 2010


A chloroplast pathway for the de novo biosynthesis of triacylglycerol in Chlamydomonas reinhardtii
journal, May 2011


Prospects for molecular farming in the green alga Chlamydomonas reinhardtii
journal, April 2004


Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardi.
journal, December 1965


Oil extraction from microalgae for biodiesel production
journal, January 2011


Chlamydomonas starchless mutant defective in ADP-glucose pyrophosphorylase hyper-accumulates triacylglycerol
journal, July 2010


Inhibition of starch synthesis results in overproduction of lipids in Chlamydomonas reinhardtii
journal, May 2010


Changes in Transcript Abundance in Chlamydomonas reinhardtii following Nitrogen Deprivation Predict Diversion of Metabolism
journal, October 2010


RNA Interference Silencing of a Major Lipid Droplet Protein Affects Lipid Droplet Size in Chlamydomonas reinhardtii
journal, November 2009


The Polyphosphate Bodies of Chlamydomonas reinhardtii Possess a Proton-pumping Pyrophosphatase and Are Similar to Acidocalcisomes
journal, September 2001


Suppression of Soybean Oleosin Produces Micro-Oil Bodies that Aggregate into Oil Body/ER Complexes
journal, November 2008


<i>Entophlyctis apiculata</i>, a chytrid parasite of <i>Chlamydomonas</i> sp. (Chlorophyceae)
journal, January 2001


Starchless Mutants of Chlamydomonas reinhardtii Lack the Small Subunit of a Heterotetrameric ADP-Glucose Pyrophosphorylase
journal, February 2001