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Title: Conditioning biomass for microbial growth

The present invention relates to methods for improving the yield of microbial processes that use lignocellulose biomass as a nutrient source. The methods comprise conditioning a composition comprising lignocellulose biomass with an enzyme composition that comprises a phenol oxidizing enzyme. The conditioned composition can support a higher rate of growth of microorganisms in a process. In one embodiment, a laccase composition is used to condition lignocellulose biomass derived from non-woody plants, such as corn and sugar cane. The invention also encompasses methods for culturing microorganisms that are sensitive to inhibitory compounds in lignocellulose biomass. The invention further provides methods of making a product by culturing the production microorganisms in conditioned lignocellulose biomass.
Issue Date:
OSTI Identifier:
Danisco US Inc. (Palo Alto, CA) GFO
Patent Number(s):
Application Number:
Contract Number:
Resource Relation:
Patent File Date: 2007 Dec 17
Research Org:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org:
Country of Publication:
United States

Other works cited in this record:

Ethanol production from enzymatic hydrolysates of sugarcane bagasse using recombinant xylose-utilising Saccharomyces cerevisiae
journal, August 2002
  • Martı́n, Carlos; Galbe, Mats; Wahlbom, C. Fredrik
  • Enzyme and Microbial Technology, Vol. 31, Issue 3, p. 274-282
  • DOI: 10.1016/S0141-0229(02)00112-6

A general method applicable to the search for similarities in the amino acid sequence of two proteins
journal, March 1970

Fermentation of lignocellulosic hydrolysates. I: inhibition and detoxification
journal, August 2000

Fermentation of lignocellulosic hydrolysates. II: inhibitors and mechanisms of inhibition
journal, August 2000

Laccase catalyzed-oxidative coupling of 3-methyl 2-benzothiazolinone hydrazone and methoxyphenols
journal, August 1999
  • Setti, Leonardo; Giuliani, Silvia; Spinozzi, Giovanni
  • Enzyme and Microbial Technology, Vol. 25, Issue 3-5, p. 285-289
  • DOI: 10.1016/S0141-0229(99)00059-9

Characterization of the secreted cellulases of Trichoderma reesei wild type and mutants during controlled fermentations
journal, July 1984
  • Sheir-Neiss, G.; Montenecourt, B. S.
  • Applied Microbiology and Biotechnology, Vol. 20, Issue 1, p. 46-53
  • DOI: 10.1007/BF00254645

Comparison of biosequences
journal, December 1981

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