Rapid classification of biological components

Thompson, Vicki S.; Barrett, Karen B.; Key, Diane E.

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Title: Rapid classification of biological components

Abstract

A method is disclosed for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an illustrative embodiment of the invention, the analyte is a drug, such as marijuana, cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method involves attaching antigens to a surface of a solid support in a preselected pattern to form an array wherein the locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to antigens in the array, thereby forming immune complexes; washing away antibodies that do not form immune complexes; and detecting the immune complexes, thereby forming an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to a subject's identity.

Inventors:: Thompson, Vicki S.; Barrett, Karen B.; Key, Diane E.

Issue Date:: 2013-10-15

Research Org.:: Idaho National Laboratory (INL), Idaho Falls, ID (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1176725

Patent Number(s):: RE44,539

Application Number:: 13/425,181

Assignee:: United States Department of Energy (Washington, DC)

DOE Contract Number:: AC07-051D14517; AC07-94ID13223; AC07-99ID13727

Resource Type:: Patent

Resource Relation:: Patent File Date: 2012 Mar 20

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats


                    Thompson, Vicki S., Barrett, Karen B., and Key, Diane E. Rapid classification of biological components.  United States: N. p., 2013. 
        Web.

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                    Thompson, Vicki S., Barrett, Karen B., & Key, Diane E. Rapid classification of biological components.  United States.

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                    Thompson, Vicki S., Barrett, Karen B., and Key, Diane E. Tue .  
        "Rapid classification of biological components".  United States.  https://www.osti.gov/servlets/purl/1176725.

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@article{osti_1176725,

  title        = {Rapid classification of biological components},

  author       = {Thompson, Vicki S. and Barrett, Karen B. and Key, Diane E.},

  abstractNote = {A method is disclosed for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an illustrative embodiment of the invention, the analyte is a drug, such as marijuana, cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method involves attaching antigens to a surface of a solid support in a preselected pattern to form an array wherein the locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to antigens in the array, thereby forming immune complexes; washing away antibodies that do not form immune complexes; and detecting the immune complexes, thereby forming an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to a subject's identity.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2013},

  month        = {10}

}

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Works referenced in this record:

Primary Antibody–Fab Fragment Complexes: A Flexible Alternative to Traditional Direct and Indirect Immunolabeling Techniques
journal, September 2004

Brown, Jeremy K.; Pemberton, Alan D.; Wright, Steven H.
Journal of Histochemistry & Cytochemistry, Vol. 52, Issue 9
https://doi.org/10.1369/jhc.3A6200.2004