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Title: Rapid classification of biological components

Abstract

A method is disclosed for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an illustrative embodiment of the invention, the analyte is a drug, such as marijuana, cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method involves attaching antigens to a surface of a solid support in a preselected pattern to form an array wherein the locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to antigens in the array, thereby forming immune complexes; washing away antibodies that do not form immune complexes; and detecting the immune complexes, thereby forming an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to a subject's identity.

Inventors:
; ;
Issue Date:
Research Org.:
Idaho National Lab. (INL), Idaho Falls, ID (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1176725
Patent Number(s):
RE44,539
Application Number:
13/425,181
Assignee:
United States Department of Energy (Washington, DC)
DOE Contract Number:  
AC07-051D14517; AC07-94ID13223; AC07-99ID13727
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 Mar 20
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats

Thompson, Vicki S., Barrett, Karen B., and Key, Diane E. Rapid classification of biological components. United States: N. p., 2013. Web.
Thompson, Vicki S., Barrett, Karen B., & Key, Diane E. Rapid classification of biological components. United States.
Thompson, Vicki S., Barrett, Karen B., and Key, Diane E. Tue . "Rapid classification of biological components". United States. https://www.osti.gov/servlets/purl/1176725.
@article{osti_1176725,
title = {Rapid classification of biological components},
author = {Thompson, Vicki S. and Barrett, Karen B. and Key, Diane E.},
abstractNote = {A method is disclosed for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an illustrative embodiment of the invention, the analyte is a drug, such as marijuana, cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method involves attaching antigens to a surface of a solid support in a preselected pattern to form an array wherein the locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to antigens in the array, thereby forming immune complexes; washing away antibodies that do not form immune complexes; and detecting the immune complexes, thereby forming an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to a subject's identity.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2013},
month = {10}
}

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