Mutant E. coli strain with increased succinic acid production
Abstract
A method for isolating succinic acid producing bacteria is provided comprising increasing the biomass of an organism which lacks the ability to catabolize pyruvate, and then subjecting the biomass to glucose-rich medium in an anaerobic environment to enable pyruvate-catabolizing mutants to grow. The invention also provides for a mutant that produces high amounts of succinic acid, which has been derived from a parent which lacked the genes for pyruvate formate lyase and lactate dehydrogenase, and which belongs to the E.coli Group of Bacteria.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of Chicago, IL (United States); Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1176700
- Patent Number(s):
- RE37393
- Application Number:
- 09/429,693; 5,770,435
- Assignee:
- The University of Chicago (Chicago, IL)
- DOE Contract Number:
- W-31-109-ENG-38
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 1999 Oct 27
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES
Citation Formats
Donnelly, Mark, Millard, Cynthia S., and Stols, Lucy. Mutant E. coli strain with increased succinic acid production. United States: N. p., 2001.
Web.
Donnelly, Mark, Millard, Cynthia S., & Stols, Lucy. Mutant E. coli strain with increased succinic acid production. United States.
Donnelly, Mark, Millard, Cynthia S., and Stols, Lucy. Tue .
"Mutant E. coli strain with increased succinic acid production". United States. https://www.osti.gov/servlets/purl/1176700.
@article{osti_1176700,
title = {Mutant E. coli strain with increased succinic acid production},
author = {Donnelly, Mark and Millard, Cynthia S. and Stols, Lucy},
abstractNote = {A method for isolating succinic acid producing bacteria is provided comprising increasing the biomass of an organism which lacks the ability to catabolize pyruvate, and then subjecting the biomass to glucose-rich medium in an anaerobic environment to enable pyruvate-catabolizing mutants to grow. The invention also provides for a mutant that produces high amounts of succinic acid, which has been derived from a parent which lacked the genes for pyruvate formate lyase and lactate dehydrogenase, and which belongs to the E.coli Group of Bacteria.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2001},
month = {9}
}