Production of polyhydroxybutyrate in switchgrass

Somleva, Mariya N.; Snell, Kristi D.; Beaulieu, Julie; Peoples, Oliver P.; Garrison, Bradley; Patterson, Nii

Title: Production of polyhydroxybutyrate in switchgrass

Abstract

Transgenic plants, plant material, and plant cells for synthesis of polyhydroxyalkanoates, preferably poly(3-hydroxybutyrate) (also referred to a as PHB) are provided. Preferred plants that can be genetically engineered to produce PHB include plants that do not normally produce storage products such as oils and carbohydrates, and plants that have a C.sub.4 NAD-malic enzyme photosynthetic pathway. Such plants also advantageously produce lignocellulosic biomass that can be converted into biofuels. An exemplary plant that can be genetically engineered to produce PHB and produce lignocellulosic biomass is switchgrass, Panicum virgatum L. A preferred cultivar of switchgrass is Alamo. Other suitable cultivars of switchgrass include but are not limited to Blackwell, Kanlow, Nebraska 28, Pathfinder, Cave-in-Rock, Shelter and Trailblazer.

Inventors:: Somleva, Mariya N.; Snell, Kristi D.; Beaulieu, Julie; Peoples, Oliver P.; Garrison, Bradley; Patterson, Nii

Issue Date:: Tue Jul 16 00:00:00 EDT 2013

Research Org.:: Metabolix, Inc., Cambridge, MA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1176503

Patent Number(s):: 8487159

Application Number:: 12/431,428

Assignee:: Metabolix, Inc. (Cambridge, MA)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/8243 - {involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine}
C12N9/0004 - {Oxidoreductases
C12N9/1025 - {Acyltransferases
C12N9/1029 - {transferring groups other than amino-acyl groups

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DOE Contract Number:: FC07-01ID14214

Resource Type:: Patent

Resource Relation:: Patent File Date: 2009 Apr 28

Country of Publication:: United States

Language:: English

Subject:: 09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Somleva, Mariya N., Snell, Kristi D., Beaulieu, Julie, Peoples, Oliver P., Garrison, Bradley, and Patterson, Nii. Production of polyhydroxybutyrate in switchgrass.  United States: N. p., 2013. 
        Web.

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                    Somleva, Mariya N., Snell, Kristi D., Beaulieu, Julie, Peoples, Oliver P., Garrison, Bradley, & Patterson, Nii. Production of polyhydroxybutyrate in switchgrass.  United States.

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                    Somleva, Mariya N., Snell, Kristi D., Beaulieu, Julie, Peoples, Oliver P., Garrison, Bradley, and Patterson, Nii. Tue .  
        "Production of polyhydroxybutyrate in switchgrass".  United States.  https://www.osti.gov/servlets/purl/1176503.

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@article{osti_1176503,

  title        = {Production of polyhydroxybutyrate in switchgrass},

  author       = {Somleva, Mariya N. and Snell, Kristi D. and Beaulieu, Julie and Peoples, Oliver P. and Garrison, Bradley and Patterson, Nii},

  abstractNote = {Transgenic plants, plant material, and plant cells for synthesis of polyhydroxyalkanoates, preferably poly(3-hydroxybutyrate) (also referred to a as PHB) are provided. Preferred plants that can be genetically engineered to produce PHB include plants that do not normally produce storage products such as oils and carbohydrates, and plants that have a C.sub.4 NAD-malic enzyme photosynthetic pathway. Such plants also advantageously produce lignocellulosic biomass that can be converted into biofuels. An exemplary plant that can be genetically engineered to produce PHB and produce lignocellulosic biomass is switchgrass, Panicum virgatum L. A preferred cultivar of switchgrass is Alamo. Other suitable cultivars of switchgrass include but are not limited to Blackwell, Kanlow, Nebraska 28, Pathfinder, Cave-in-Rock, Shelter and Trailblazer.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Jul 16 00:00:00 EDT 2013},

  month        = {Tue Jul 16 00:00:00 EDT 2013}

}

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Schmidt, J.; Bubunenko, M.; Subramanian, A. R.
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Soybean transformation by microparticle bombardment
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Metabolic pathway engineering in cotton: Biosynthesis of polyhydroxybutyrate in fiber cells
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John, M. E.; Keller, G.
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Molecular breeding of switchgrass for use as a biofuel crop
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Bouton, Joseph H.
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Tetracycline-regulated transcriptional inhibitors
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Bujard, Hermann; Gossen, Manfred
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Light-responsive elements of the tobacco PSI-D gene are located both upstream and within the transcribed region
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Yamamoto, Yoshiharu Y.; Kondo, Yukiko; Kato, Akira
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Transgenic expression of aminoglycoside adenine transferase in the chloroplast: a selectable marker for site-directed transformation of chlamydomonas
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Goldschmidt-Clermont, Michel
Nucleic Acids Research, Vol. 19, Issue 15
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Poly(β-hydroxybutyrate) production in oilseed leukoplasts of Brassica napus
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Houmiel, Kathryn L.; Slater, Steven; Broyles, Debra
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Insecticidal cotton plants
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Barton, Kenneth; Umbeck, Paul F.
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Plant group 2 promoters and uses thereof
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Net energy of cellulosic ethanol from switchgrass
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Schmer, M. R.; Vogel, K. P.; Mitchell, R. B.
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Particle gun
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Tomes, Dwight
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Transgenic maize plants by tissue electroporation.
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D'Halluin, K.; Bonne, E.; Bossut, M.
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Peoples, O. P.; Sinskey, A. J.
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ALS3 promoter
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Baszczynski, Chris; Barbour, Eric; Miki, Brian
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Synthetic promoters
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Signal peptide analogs derived from two chloroplast precursors interact with the signal recognition system of the chloroplast envelope.
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Schnell, D. J.; Blobel, G.; Pain, D.
Journal of Biological Chemistry, Vol. 266, Issue 5
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Switchgrass as a biofuels feedstock in the USA
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Sanderson, M. A.; Adler, P. R.; Boateng, A. A.
Canadian Journal of Plant Science, Vol. 86, Issue Special Issue
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Transfer of foreign genes into intact maize cells with high-velocity microprojectiles
journal, June 1988

Klein, T. M.; Fromm, M.; Weissinger, A.
Proceedings of the National Academy of Sciences, Vol. 85, Issue 12
https://doi.org/10.1073/pnas.85.12.4305

Production of Polyhydroxybutyrate by Polycistronic Expression of Bacterial Genes in Tobacco Plastid
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Arai, Yuko; Shikanai, Toshiharu; Doi, Yoshiharu
Plant and Cell Physiology, Vol. 45, Issue 9
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Pathogen-resistant transgenic plants
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Conkling, Mark; Opperman, Charles H.; Taylor, Christopher
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Spatio-temporal characterization of polyhydroxybutyrate accumulation in sugarcane
journal, January 2007

Purnell, Matthew P.; Petrasovits, Lars A.; Nielsen, Lars K.
Plant Biotechnology Journal, Vol. 5, Issue 1
https://doi.org/10.1111/j.1467-7652.2006.00230.x

Analysis of three tissue-specific elements from the wheat Cab-1 enhancer: Functional elements of a Cab enhancer
journal, April 1993

Gotor, Cecilia; Romero, Luis C.; Inouye, Keiko
The Plant Journal, Vol. 3, Issue 4
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Method for stably transforming plastids of multicellular plants
patent, September 1995

Maliga, Pal; Maliga, Zora S.
US Patent Document 5,451,513
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5451513

Mannose or xylose based positive selection
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Bojsen, Kirsten; Donaldson, Iain; Haldrup, Anna
US Patent Document 5,767,378
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Stable transformation of tobacco by electroporation: evidence for plasmid concatenation.
journal, August 1986

Riggs, C. D.; Bates, G. W.
Proceedings of the National Academy of Sciences, Vol. 83, Issue 15
https://doi.org/10.1073/pnas.83.15.5602

Accumulation of D1 polypeptide in tobacco plastids is regulated via the untranslated region of the psbA mRNA.
journal, February 1993

Staub, J. M.; Maliga, P.
The EMBO Journal, Vol. 12, Issue 2
https://doi.org/10.1002/j.1460-2075.1993.tb05692.x

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Title: Production of polyhydroxybutyrate in switchgrass

Abstract

Citation Formats

Strcuture and transcription of the nopaline synthase gene region of T-DNA journal, January 1983

Modification of the coding sequence enhances plant expression of insect control protein genes. journal, April 1991

Polyester synthesis in transplastomic tobacco (Nicotiana tabacum L.): significant contents of polyhydroxybutyrate are associated with growth reduction journal, April 2003

PHA production, from bacteria to plants journal, June 1999

Method for producing transgenic cereal plants patent, April 1998

Methods for regulating gene expression patent, September 1998

Polyhydroxybutyrate synthesis in transgenic flax journal, January 2004

High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene. journal, February 1993

Proteins with insecticidal properties against homopteran insects and their use in plant protection patent, February 1997

Expression of bacterial poly(3-hydroxybutyrate) synthesis genes in hairy roots of sugar beet (Beta vulgaris L.) journal, January 2003

Selective gene expression in plants patent, May 1992

Method of Agrobacterium-mediated transformation of cultured soybean cells patent, October 1996

Engineering of PHB Synthesis Causes Improved Elastic Properties of Flax Fibers journal, February 2007

Production of polyhydroxybutyrate in sugarcane journal, January 2007

Bonkers about biofuels journal, July 2006

Root preferential promoter patent, May 1997

Vascular-specific expression of the bean GRP 1.8 gene is negatively regulated. journal, October 1991

Tissue-specific and wound-inducible pattern of expression of the mannopine synthase promoter is determined by the interaction between positive and negative cis-regulatory elements journal, September 1993

Construction of a GFP-BAR plasmid and its use for switchgrass transformation journal, January 2001

Tissue-preferential promoters patent, November 1995

A novel thiolase-reductase gene fusion promotes the production of polyhydroxybutyrate in Arabidopsis journal, July 2005

Alterations in the Chlamydomonas Plastocyanin Transit Peptide Have Distinct Effects on in VitroImport and in Vivo Protein Accumulation journal, August 1997

Polyhydroxybutyrate polymerase patent, July 1996

Tissue-Specific Activity of Two Manganese Superoxide Dismutase Promoters in Transgenic Tobacco journal, October 1996

Automatic SMD tester patent, July 1990

Isolation of an efficient actin promoter for use in rice transformation. journal, February 1990

Methods of regeneration of Medicago sativa and expressing foreign DNA in same patent, June 1994

The chlorophyll a/b-binding protein inserts into the thylakoids independent of its cognate transit peptide. journal, October 1988

Method for producing polyester biopolymers patent, September 1997

Metabolic Engineering: Plastids as Bioreactors book, January 2004

Biological systems for manufacture of polyhydroxyalkanoate polymers containing 4-hydroxyacids patent, November 2001

Protein Trafficking in Plant Cells journal, August 1987

Transgenic Arabidopsis plants can accumulate polyhydroxybutyrate to up to 4% of their fresh weight journal, November 2000

Metabolic Engineering of Poly(3-Hydroxyalkanoates): From DNA to Plastic journal, January 1999

Nuclear Genes Encoding the Small Subunit of Ribulose-1,5-Bisphosphate Carboxylase book, January 1983

Constitutive Expression of the β-Ketothiolase Gene in Transgenic Plants. A Major Obstacle for Obtaining Polyhydroxybutyrate-Producing Plants journal, February 2002

Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter journal, February 1985

Metabolic engineering of Arabidopsis and Brassica for poly(3-hydroxybutyrate- co-3-hydroxyvalerate) copolymer production journal, October 1999

Synthetic insecticidal crystal protein gene patent, January 1995

Stable transformation of plastids in higher plants. journal, November 1990

Tissue-Specific Expression and Promoter Analysis of the Tobacco ltp1 Gene journal, October 1996

Biotechnological approaches for the production of polyhydroxyalkanoates in microorganisms and plants — A review journal, March 2007

Root cortex specific gene promoter patent, November 1998

Switchgrass (Panicum virgatum L.) book, January 2006

Controlled expression of plastid transgenes in plants based on a nuclear DNA-encoded and plastid-targeted T7 RNA polymerase. journal, July 1994

Luciferase reporter gene cassettes for plant gene expression studies journal, January 1987

CELLULOSIC ETHANOL: Biofuel Researchers Prepare to Reap a New Harvest journal, March 2007

A steroid-inducible gene expression system for plant cells. journal, December 1991

Long regions of homologous DNA are incorporated into the tobacco plastid genome by transformation. journal, January 1992

Plant promoter .alpha.-glucuronidase gene construct patent, December 1993

Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana results in high levels of polymer accumulation. journal, December 1994

Tissue-Specific and Developmental Regulation of Cotton Gene FbL2A (Demonstration of Promoter Activity in Transgenic Plants) journal, November 1996

Chemically inducible expression of the PHB biosynthetic pathway in Arabidopsis journal, February 2007

Rice chitinase promoter patent, March 1995

Introduction of Bacterial Metabolism into Higher Plants by Polycistronic Transgene Expression journal, January 2001

Immunological Characterization and Chloroplast Localization of the Tryptophan Biosynthetic Enzymes of the Flowering Plant Arabidopsis thaliana journal, March 1995

Inducible Trans-activation of Plastid Transgenes: Expression of the R. eutrophaphb Operon in Transplastomic Tobacco journal, September 2005

-Mediated Genetic Transformation of Switchgrass journal, January 2002

Enhanced expression in a plant plastid patent, August 1996

Promoter enhancer element for gene expression in plant roots patent, June 1991

Expression of Bacillus thuringiensis cry proteins in plant plastids patent, August 1996

Plastid Targeting of Polyhydroxybutyrate Biosynthetic Pathway in Tobacco journal, January 2001

T-DNA organization in tumor cultures and transgenic plants of the monocotyledon Asparagus officinalis journal, August 1987

Stable Transformation of Soybean Callus by DNA-Coated Gold Particles journal, July 1988

Brassica regulatory sequence for root-specific or root-abundant gene expression patent, March 1995

Ammonia-regulated expression of a soybean gene encoding cytosolic glutamine synthetase in transgenic Lotus corniculatus. journal, January 1991

Genes for the synthesis of antipathogenic substances patent, June 1997

Glyphosate-resistant plants patent, February 1993

Methods of inserting viral DNA into plant material patent, October 1996

Production of a Biodegradable Plastic Polymer, Poly‐β‐Hydroxybutyrate, in Transgenic Alfalfa journal, May 2002

Mutations at the Transit Peptide-Mature Protein Junction Separate Two Cleavage Events during Chloroplast Import of the Chlorophyll a/b-binding Protein journal, October 1989

Transactivation of anthocyanin biosynthetic genes following transfer of B regulatory genes into maize tissues. journal, August 1990

Parameters Influencing Stable Transformation of Rice Immature Embryos and Recovery of Transgenic Plants using Electric Discharge Particle Acceleration journal, April 1995

Tissue-specific light-regulated expression directed by the promoter of a C4 gene, maize pyruvate,orthophosphate dikinase, in a C3 plant, rice. journal, October 1993

The Biology and Agronomy of Switchgrass for Biofuels journal, September 2005

Genetic Transformation of Maize Cells by Particle Bombardment journal, September 1989

Inheritance and Expression of Chimeric Genes in the Progeny of Transgenic Maize Plants journal, September 1990

Strcuture and transcription of the nopaline synthase gene region of T-DNA
journal, January 1983

Modification of the coding sequence enhances plant expression of insect control protein genes.
journal, April 1991

Polyester synthesis in transplastomic tobacco (Nicotiana tabacum L.): significant contents of polyhydroxybutyrate are associated with growth reduction
journal, April 2003

PHA production, from bacteria to plants
journal, June 1999

Method for producing transgenic cereal plants
patent, April 1998

Methods for regulating gene expression
patent, September 1998

Polyhydroxybutyrate synthesis in transgenic flax
journal, January 2004

High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene.
journal, February 1993

Proteins with insecticidal properties against homopteran insects and their use in plant protection
patent, February 1997

Expression of bacterial poly(3-hydroxybutyrate) synthesis genes in hairy roots of sugar beet (Beta vulgaris L.)
journal, January 2003

Selective gene expression in plants
patent, May 1992

Method of Agrobacterium-mediated transformation of cultured soybean cells
patent, October 1996

Engineering of PHB Synthesis Causes Improved Elastic Properties of Flax Fibers
journal, February 2007

Production of polyhydroxybutyrate in sugarcane
journal, January 2007

Bonkers about biofuels
journal, July 2006

Root preferential promoter
patent, May 1997

Vascular-specific expression of the bean GRP 1.8 gene is negatively regulated.
journal, October 1991

Tissue-specific and wound-inducible pattern of expression of the mannopine synthase promoter is determined by the interaction between positive and negative cis-regulatory elements
journal, September 1993

Construction of a GFP-BAR plasmid and its use for switchgrass transformation
journal, January 2001

Tissue-preferential promoters
patent, November 1995

A novel thiolase-reductase gene fusion promotes the production of polyhydroxybutyrate in Arabidopsis
journal, July 2005

Alterations in the Chlamydomonas Plastocyanin Transit Peptide Have Distinct Effects on in VitroImport and in Vivo Protein Accumulation
journal, August 1997

Polyhydroxybutyrate polymerase
patent, July 1996

Tissue-Specific Activity of Two Manganese Superoxide Dismutase Promoters in Transgenic Tobacco
journal, October 1996

Automatic SMD tester
patent, July 1990

Isolation of an efficient actin promoter for use in rice transformation.
journal, February 1990

Methods of regeneration of Medicago sativa and expressing foreign DNA in same
patent, June 1994

The chlorophyll a/b-binding protein inserts into the thylakoids independent of its cognate transit peptide.
journal, October 1988

Method for producing polyester biopolymers
patent, September 1997

Metabolic Engineering: Plastids as Bioreactors
book, January 2004

Biological systems for manufacture of polyhydroxyalkanoate polymers containing 4-hydroxyacids
patent, November 2001

Protein Trafficking in Plant Cells
journal, August 1987

Transgenic Arabidopsis plants can accumulate polyhydroxybutyrate to up to 4% of their fresh weight
journal, November 2000

Metabolic Engineering of Poly(3-Hydroxyalkanoates): From DNA to Plastic
journal, January 1999

Nuclear Genes Encoding the Small Subunit of Ribulose-1,5-Bisphosphate Carboxylase
book, January 1983

Constitutive Expression of the β-Ketothiolase Gene in Transgenic Plants. A Major Obstacle for Obtaining Polyhydroxybutyrate-Producing Plants
journal, February 2002

Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter
journal, February 1985

Metabolic engineering of Arabidopsis and Brassica for poly(3-hydroxybutyrate- co-3-hydroxyvalerate) copolymer production
journal, October 1999

Synthetic insecticidal crystal protein gene
patent, January 1995

Stable transformation of plastids in higher plants.
journal, November 1990

Tissue-Specific Expression and Promoter Analysis of the Tobacco ltp1 Gene
journal, October 1996

Biotechnological approaches for the production of polyhydroxyalkanoates in microorganisms and plants — A review
journal, March 2007

Root cortex specific gene promoter
patent, November 1998

Switchgrass (Panicum virgatum L.)
book, January 2006

Controlled expression of plastid transgenes in plants based on a nuclear DNA-encoded and plastid-targeted T7 RNA polymerase.
journal, July 1994

Luciferase reporter gene cassettes for plant gene expression studies
journal, January 1987

CELLULOSIC ETHANOL: Biofuel Researchers Prepare to Reap a New Harvest
journal, March 2007

A steroid-inducible gene expression system for plant cells.
journal, December 1991

Long regions of homologous DNA are incorporated into the tobacco plastid genome by transformation.
journal, January 1992

Plant promoter .alpha.-glucuronidase gene construct
patent, December 1993

Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana results in high levels of polymer accumulation.
journal, December 1994

Tissue-Specific and Developmental Regulation of Cotton Gene FbL2A (Demonstration of Promoter Activity in Transgenic Plants)
journal, November 1996

Chemically inducible expression of the PHB biosynthetic pathway in Arabidopsis
journal, February 2007

Rice chitinase promoter
patent, March 1995

Introduction of Bacterial Metabolism into Higher Plants by Polycistronic Transgene Expression
journal, January 2001

Immunological Characterization and Chloroplast Localization of the Tryptophan Biosynthetic Enzymes of the Flowering Plant Arabidopsis thaliana
journal, March 1995

Inducible Trans-activation of Plastid Transgenes: Expression of the R. eutrophaphb Operon in Transplastomic Tobacco
journal, September 2005

-Mediated Genetic Transformation of Switchgrass
journal, January 2002

Enhanced expression in a plant plastid
patent, August 1996

Promoter enhancer element for gene expression in plant roots
patent, June 1991

Expression of Bacillus thuringiensis cry proteins in plant plastids
patent, August 1996

Plastid Targeting of Polyhydroxybutyrate Biosynthetic Pathway in Tobacco
journal, January 2001

T-DNA organization in tumor cultures and transgenic plants of the monocotyledon Asparagus officinalis
journal, August 1987

Stable Transformation of Soybean Callus by DNA-Coated Gold Particles
journal, July 1988

Brassica regulatory sequence for root-specific or root-abundant gene expression
patent, March 1995

Ammonia-regulated expression of a soybean gene encoding cytosolic glutamine synthetase in transgenic Lotus corniculatus.
journal, January 1991

Genes for the synthesis of antipathogenic substances
patent, June 1997

Glyphosate-resistant plants
patent, February 1993

Methods of inserting viral DNA into plant material
patent, October 1996

Production of a Biodegradable Plastic Polymer, Poly‐β‐Hydroxybutyrate, in Transgenic Alfalfa
journal, May 2002

Mutations at the Transit Peptide-Mature Protein Junction Separate Two Cleavage Events during Chloroplast Import of the Chlorophyll a/b-binding Protein
journal, October 1989

Transactivation of anthocyanin biosynthetic genes following transfer of B regulatory genes into maize tissues.
journal, August 1990

Parameters Influencing Stable Transformation of Rice Immature Embryos and Recovery of Transgenic Plants using Electric Discharge Particle Acceleration
journal, April 1995

Tissue-specific light-regulated expression directed by the promoter of a C4 gene, maize pyruvate,orthophosphate dikinase, in a C3 plant, rice.
journal, October 1993

The Biology and Agronomy of Switchgrass for Biofuels
journal, September 2005

Genetic Transformation of Maize Cells by Particle Bombardment
journal, September 1989

Inheritance and Expression of Chimeric Genes in the Progeny of Transgenic Maize Plants
journal, September 1990

Glucocorticoid-inducible expression of a bacterial avirulence gene in transgenic Arabidopsis induces hypersensitive cell death: Glucocorticoid-inducible cell death
journal, April 1998

Plant Regeneraton from Callus Cultures of Switchgrass
journal, January 1994

Enhanced expression in plants
patent, January 1997