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Title: Production of polyhydroxybutyrate in switchgrass

Abstract

Transgenic plants, plant material, and plant cells for synthesis of polyhydroxyalkanoates, preferably poly(3-hydroxybutyrate) (also referred to a as PHB) are provided. Preferred plants that can be genetically engineered to produce PHB include plants that do not normally produce storage products such as oils and carbohydrates, and plants that have a C.sub.4 NAD-malic enzyme photosynthetic pathway. Such plants also advantageously produce lignocellulosic biomass that can be converted into biofuels. An exemplary plant that can be genetically engineered to produce PHB and produce lignocellulosic biomass is switchgrass, Panicum virgatum L. A preferred cultivar of switchgrass is Alamo. Other suitable cultivars of switchgrass include but are not limited to Blackwell, Kanlow, Nebraska 28, Pathfinder, Cave-in-Rock, Shelter and Trailblazer.

Inventors:
; ; ; ; ;
Issue Date:
Research Org.:
Metabolix, Inc., Cambridge, MA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1176503
Patent Number(s):
8487159
Application Number:
12/431,428
Assignee:
Metabolix, Inc. (Cambridge, MA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FC07-01ID14214
Resource Type:
Patent
Resource Relation:
Patent File Date: 2009 Apr 28
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Somleva, Mariya N., Snell, Kristi D., Beaulieu, Julie, Peoples, Oliver P., Garrison, Bradley, and Patterson, Nii. Production of polyhydroxybutyrate in switchgrass. United States: N. p., 2013. Web.
Somleva, Mariya N., Snell, Kristi D., Beaulieu, Julie, Peoples, Oliver P., Garrison, Bradley, & Patterson, Nii. Production of polyhydroxybutyrate in switchgrass. United States.
Somleva, Mariya N., Snell, Kristi D., Beaulieu, Julie, Peoples, Oliver P., Garrison, Bradley, and Patterson, Nii. Tue . "Production of polyhydroxybutyrate in switchgrass". United States. https://www.osti.gov/servlets/purl/1176503.
@article{osti_1176503,
title = {Production of polyhydroxybutyrate in switchgrass},
author = {Somleva, Mariya N. and Snell, Kristi D. and Beaulieu, Julie and Peoples, Oliver P. and Garrison, Bradley and Patterson, Nii},
abstractNote = {Transgenic plants, plant material, and plant cells for synthesis of polyhydroxyalkanoates, preferably poly(3-hydroxybutyrate) (also referred to a as PHB) are provided. Preferred plants that can be genetically engineered to produce PHB include plants that do not normally produce storage products such as oils and carbohydrates, and plants that have a C.sub.4 NAD-malic enzyme photosynthetic pathway. Such plants also advantageously produce lignocellulosic biomass that can be converted into biofuels. An exemplary plant that can be genetically engineered to produce PHB and produce lignocellulosic biomass is switchgrass, Panicum virgatum L. A preferred cultivar of switchgrass is Alamo. Other suitable cultivars of switchgrass include but are not limited to Blackwell, Kanlow, Nebraska 28, Pathfinder, Cave-in-Rock, Shelter and Trailblazer.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2013},
month = {7}
}

Works referenced in this record:

Strcuture and transcription of the nopaline synthase gene region of T-DNA
journal, January 1983


Modification of the coding sequence enhances plant expression of insect control protein genes.
journal, April 1991


PHA production, from bacteria to plants
journal, June 1999


Method for producing transgenic cereal plants
patent, April 1998


Methods for regulating gene expression
patent, September 1998


Polyhydroxybutyrate synthesis in transgenic flax
journal, January 2004


High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene.
journal, February 1993


Proteins with insecticidal properties against homopteran insects and their use in plant protection
patent, February 1997


Expression of bacterial poly(3-hydroxybutyrate) synthesis genes in hairy roots of sugar beet (Beta vulgaris L.)
journal, January 2003


Selective gene expression in plants
patent, May 1992


Engineering of PHB Synthesis Causes Improved Elastic Properties of Flax Fibers
journal, February 2007


Production of polyhydroxybutyrate in sugarcane
journal, January 2007


Bonkers about biofuels
journal, July 2006


Root preferential promoter
patent, May 1997


Vascular-specific expression of the bean GRP 1.8 gene is negatively regulated.
journal, October 1991


Construction of a GFP-BAR plasmid and its use for switchgrass transformation
journal, January 2001


Tissue-preferential promoters
patent, November 1995


A novel thiolase-reductase gene fusion promotes the production of polyhydroxybutyrate in Arabidopsis
journal, July 2005


Polyhydroxybutyrate polymerase
patent, July 1996


Tissue-Specific Activity of Two Manganese Superoxide Dismutase Promoters in Transgenic Tobacco
journal, October 1996


Automatic SMD tester
patent, July 1990


Isolation of an efficient actin promoter for use in rice transformation.
journal, February 1990


Methods of regeneration of Medicago sativa and expressing foreign DNA in same
patent, June 1994


Method for producing polyester biopolymers
patent, September 1997


Metabolic Engineering: Plastids as Bioreactors
book, January 2004


Biological systems for manufacture of polyhydroxyalkanoate polymers containing 4-hydroxyacids
patent, November 2001


Protein Trafficking in Plant Cells
journal, August 1987


Transgenic Arabidopsis plants can accumulate polyhydroxybutyrate to up to 4% of their fresh weight
journal, November 2000


Metabolic Engineering of Poly(3-Hydroxyalkanoates): From DNA to Plastic
journal, January 1999


Constitutive Expression of the β-Ketothiolase Gene in Transgenic Plants. A Major Obstacle for Obtaining Polyhydroxybutyrate-Producing Plants
journal, February 2002


Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter
journal, February 1985


Metabolic engineering of Arabidopsis and Brassica for poly(3-hydroxybutyrate-  co-3-hydroxyvalerate) copolymer production
journal, October 1999


Synthetic insecticidal crystal protein gene
patent, January 1995


Stable transformation of plastids in higher plants.
journal, November 1990


Tissue-Specific Expression and Promoter Analysis of the Tobacco ltp1 Gene
journal, October 1996


Biotechnological approaches for the production of polyhydroxyalkanoates in microorganisms and plants — A review
journal, March 2007


Root cortex specific gene promoter
patent, November 1998


Switchgrass (Panicum virgatum L.)
book, January 2006


Controlled expression of plastid transgenes in plants based on a nuclear DNA-encoded and plastid-targeted T7 RNA polymerase.
journal, July 1994


Luciferase reporter gene cassettes for plant gene expression studies
journal, January 1987


A steroid-inducible gene expression system for plant cells.
journal, December 1991


Plant promoter .alpha.-glucuronidase gene construct
patent, December 1993


Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana results in high levels of polymer accumulation.
journal, December 1994


Tissue-Specific and Developmental Regulation of Cotton Gene FbL2A (Demonstration of Promoter Activity in Transgenic Plants)
journal, November 1996


Chemically inducible expression of the PHB biosynthetic pathway in Arabidopsis
journal, February 2007


Rice chitinase promoter
patent, March 1995


Introduction of Bacterial Metabolism into Higher Plants by Polycistronic Transgene Expression
journal, January 2001


Inducible Trans-activation of Plastid Transgenes: Expression of the R. eutrophaphb Operon in Transplastomic Tobacco
journal, September 2005


-Mediated Genetic Transformation of Switchgrass
journal, January 2002


Enhanced expression in a plant plastid
patent, August 1996


Promoter enhancer element for gene expression in plant roots
patent, June 1991


Plastid Targeting of Polyhydroxybutyrate Biosynthetic Pathway in Tobacco
journal, January 2001


T-DNA organization in tumor cultures and transgenic plants of the monocotyledon Asparagus officinalis
journal, August 1987


Stable Transformation of Soybean Callus by DNA-Coated Gold Particles
journal, July 1988


Brassica regulatory sequence for root-specific or root-abundant gene expression
patent, March 1995


Genes for the synthesis of antipathogenic substances
patent, June 1997


Glyphosate-resistant plants
patent, February 1993


Methods of inserting viral DNA into plant material
patent, October 1996


Production of a Biodegradable Plastic Polymer, Poly‐β‐Hydroxybutyrate, in Transgenic Alfalfa
journal, May 2002


Tissue-specific light-regulated expression directed by the promoter of a C4 gene, maize pyruvate,orthophosphate dikinase, in a C3 plant, rice.
journal, October 1993


The Biology and Agronomy of Switchgrass for Biofuels
journal, September 2005


Genetic Transformation of Maize Cells by Particle Bombardment
journal, September 1989


Inheritance and Expression of Chimeric Genes in the Progeny of Transgenic Maize Plants
journal, September 1990


Plant Regeneraton from Callus Cultures of Switchgrass
journal, January 1994


Enhanced expression in plants
patent, January 1997


Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cells
journal, December 1984


Root specific gene promoter
patent, October 1995


The Path Forward for Biofuels and Biomaterials
journal, January 2006


A cassette containing the bar gene of Streptomyces hygroscopicus : a selectable marker for plant transformation
journal, January 1990


Soybean transformation by microparticle bombardment
patent, June 1994


Firefly luciferase gene: structure and expression in mammalian cells
journal, February 1987


Metabolic pathway engineering in cotton: Biosynthesis of polyhydroxybutyrate in fiber cells
journal, November 1996


Molecular breeding of switchgrass for use as a biofuel crop
journal, December 2007


Tetracycline-regulated transcriptional inhibitors
patent, August 1998


Light-responsive elements of the tobacco PSI-D gene are located both upstream and within the transcribed region
journal, August 1997


Poly(β-hydroxybutyrate) production in oilseed leukoplasts of Brassica napus
journal, October 1999


Insecticidal cotton plants
patent, March 1997


Plant group 2 promoters and uses thereof
patent, March 1997


Net energy of cellulosic ethanol from switchgrass
journal, January 2008


Multigene expression vectors for the biosynthesis of products via multienzyme biological pathways
patent, September 2002


Particle gun
patent, August 1993


Method for transporting substances into living cells and tissues and apparatus therefor
patent, July 1990


Chemically inducible expression of biosynthetic pathways
patent, June 2010


Transgenic maize plants by tissue electroporation.
journal, December 1992


Poly-β-hydroxybutyrate biosynthesis in Alcaligenes eutrophus H16
journal, September 1989


ALS3 promoter
patent, August 1997


Synthetic promoters
patent, June 2000


Switchgrass as a biofuels feedstock in the USA
journal, December 2006


Transfer of foreign genes into intact maize cells with high-velocity microprojectiles
journal, June 1988


Production of Polyhydroxybutyrate by Polycistronic Expression of Bacterial Genes in Tobacco Plastid
journal, September 2004


Pathogen-resistant transgenic plants
patent, May 1998


Spatio-temporal characterization of polyhydroxybutyrate accumulation in sugarcane
journal, January 2007


Method for stably transforming plastids of multicellular plants
patent, September 1995


Mannose or xylose based positive selection
patent, June 1998


Stable transformation of tobacco by electroporation: evidence for plasmid concatenation.
journal, August 1986