Detection of protease and protease activity using a single nanoscrescent SERS probe
Abstract
This invention pertains to the in vitro detection of proteases using a single peptide-conjugate nanocrescent surface enhanced Raman scattering (SERS) probes with at least nanomolar sensitivity. The probe enables detection of proteolytic activity in extremely small volume and at low concentration. In certain embodiments the probes comprise an indicator for the detection of an active protease, where the indicator comprises a nanocrescent attached to a peptide, where said peptide comprises a recognition site for the protease and a Raman tag attached to the peptide.
- Inventors:
- Issue Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1176495
- Patent Number(s):
- 8361932
- Application Number:
- 12/299,284
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- AC02-05CH11231
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2007 May 02
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; 47 OTHER INSTRUMENTATION
Citation Formats
Liu, Gang L., Ellman, Jonathan A., Lee, Luke P., and Chen, Fanqing Frank. Detection of protease and protease activity using a single nanoscrescent SERS probe. United States: N. p., 2013.
Web.
Liu, Gang L., Ellman, Jonathan A., Lee, Luke P., & Chen, Fanqing Frank. Detection of protease and protease activity using a single nanoscrescent SERS probe. United States.
Liu, Gang L., Ellman, Jonathan A., Lee, Luke P., and Chen, Fanqing Frank. Tue .
"Detection of protease and protease activity using a single nanoscrescent SERS probe". United States. https://www.osti.gov/servlets/purl/1176495.
@article{osti_1176495,
title = {Detection of protease and protease activity using a single nanoscrescent SERS probe},
author = {Liu, Gang L. and Ellman, Jonathan A. and Lee, Luke P. and Chen, Fanqing Frank},
abstractNote = {This invention pertains to the in vitro detection of proteases using a single peptide-conjugate nanocrescent surface enhanced Raman scattering (SERS) probes with at least nanomolar sensitivity. The probe enables detection of proteolytic activity in extremely small volume and at low concentration. In certain embodiments the probes comprise an indicator for the detection of an active protease, where the indicator comprises a nanocrescent attached to a peptide, where said peptide comprises a recognition site for the protease and a Raman tag attached to the peptide.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 29 00:00:00 EST 2013},
month = {Tue Jan 29 00:00:00 EST 2013}
}
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