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Title: Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine

Abstract

The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the unnatural amino acid phenylselenocysteine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal aminoacyl-tRNA synthetases, polynucleotides encoding the novel synthetase molecules, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid phenylselenocysteine and translation systems. The invention further provides methods for producing modified proteins (e.g., lipidated proteins) through targeted modification of the phenylselenocysteine residue in a protein.

Inventors:
;
Issue Date:
Research Org.:
The Scripps Research Inst., La Jolla, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1176457
Patent Number(s):
8217145
Application Number:
12/225,261
Assignee:
Scripps Research Institute (La Jolla, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
FG02-03ER46051; FG03-00ER45812
Resource Type:
Patent
Resource Relation:
Patent File Date: 2007 Mar 13
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Wang, Jiangyun, and Schultz, Peter G. Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine. United States: N. p., 2012. Web.
Wang, Jiangyun, & Schultz, Peter G. Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine. United States.
Wang, Jiangyun, and Schultz, Peter G. Tue . "Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine". United States. https://www.osti.gov/servlets/purl/1176457.
@article{osti_1176457,
title = {Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine},
author = {Wang, Jiangyun and Schultz, Peter G.},
abstractNote = {The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the unnatural amino acid phenylselenocysteine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal aminoacyl-tRNA synthetases, polynucleotides encoding the novel synthetase molecules, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid phenylselenocysteine and translation systems. The invention further provides methods for producing modified proteins (e.g., lipidated proteins) through targeted modification of the phenylselenocysteine residue in a protein.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2012},
month = {7}
}

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Works referenced in this record:

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