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Title: Xylitol synthesis mutant of xylose-utilizing zymomonas for ethanol production

Abstract

A strain of xylose-utilizing Zymomonas was engineered with a genetic modification to the glucose-fructose oxidoreductase gene resulting in reduced expression of GFOR enzyme activity. The engineered strain exhibits reduced production of xylitol, a detrimental by-product of xylose metabolism. It also consumes more xylose and produces more ethanol during mixed sugar fermentation under process-relevant conditions.

Inventors:
; ; ;
Issue Date:
Research Org.:
E. I. du Pont de Nemours and Company, Wilmington, DE (United States); Alliance for Sustainable Energy LLC, Golden, CO (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1176384
Patent Number(s):
7741119
Application Number:
11/862,566
Assignee:
E. I. du Pont de Nemours and Company (Wilmington, DE); Alliance for Sustainable Energy LLC (Golden, CO)
Patent Classifications (CPCs):
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FC36-03GO13146
Resource Type:
Patent
Resource Relation:
Patent File Date: 2007 Sep 27
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS

Citation Formats

Viitanen, Paul V., Chou, Yat-Chen, McCutchen, Carol M., and Zhang, Min. Xylitol synthesis mutant of xylose-utilizing zymomonas for ethanol production. United States: N. p., 2010. Web.
Viitanen, Paul V., Chou, Yat-Chen, McCutchen, Carol M., & Zhang, Min. Xylitol synthesis mutant of xylose-utilizing zymomonas for ethanol production. United States.
Viitanen, Paul V., Chou, Yat-Chen, McCutchen, Carol M., and Zhang, Min. Tue . "Xylitol synthesis mutant of xylose-utilizing zymomonas for ethanol production". United States. https://www.osti.gov/servlets/purl/1176384.
@article{osti_1176384,
title = {Xylitol synthesis mutant of xylose-utilizing zymomonas for ethanol production},
author = {Viitanen, Paul V. and Chou, Yat-Chen and McCutchen, Carol M. and Zhang, Min},
abstractNote = {A strain of xylose-utilizing Zymomonas was engineered with a genetic modification to the glucose-fructose oxidoreductase gene resulting in reduced expression of GFOR enzyme activity. The engineered strain exhibits reduced production of xylitol, a detrimental by-product of xylose metabolism. It also consumes more xylose and produces more ethanol during mixed sugar fermentation under process-relevant conditions.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {6}
}

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Works referenced in this record:

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