Ethanol production using xylitol synthesis mutant of xylose-utilizing zymomonas
Abstract
Production of ethanol using a strain of xylose-utilizing Zymomonas with a genetic modification of the glucose-fructose oxidoreductase gene was found to be improved due to greatly reduced production of xylitol, a detrimental by-product of xylose metabolism synthesized during fermentation.
- Inventors:
- Issue Date:
- Research Org.:
- E. I. du Pont de Nemours and Company, Wilmington, DE (United States); Alliance for Sustainable Energy LLC, Golden, CO (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1176383
- Patent Number(s):
- 7741084
- Application Number:
- 11/862,736
- Assignee:
- E. I. du Pont de Nemours and Company (Wilmington, DE); Alliance for Sustainable Energy LLC (Golden, CO)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- DOE Contract Number:
- FC36-03GO13146
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2007 Sep 27
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS
Citation Formats
Viitanen, Paul V., McCutchen, Carol M., Emptage, Mark, Caimi, Perry G., Zhang, Min, and Chou, Yat-Chen. Ethanol production using xylitol synthesis mutant of xylose-utilizing zymomonas. United States: N. p., 2010.
Web.
Viitanen, Paul V., McCutchen, Carol M., Emptage, Mark, Caimi, Perry G., Zhang, Min, & Chou, Yat-Chen. Ethanol production using xylitol synthesis mutant of xylose-utilizing zymomonas. United States.
Viitanen, Paul V., McCutchen, Carol M., Emptage, Mark, Caimi, Perry G., Zhang, Min, and Chou, Yat-Chen. Tue .
"Ethanol production using xylitol synthesis mutant of xylose-utilizing zymomonas". United States. https://www.osti.gov/servlets/purl/1176383.
@article{osti_1176383,
title = {Ethanol production using xylitol synthesis mutant of xylose-utilizing zymomonas},
author = {Viitanen, Paul V. and McCutchen, Carol M. and Emptage, Mark and Caimi, Perry G. and Zhang, Min and Chou, Yat-Chen},
abstractNote = {Production of ethanol using a strain of xylose-utilizing Zymomonas with a genetic modification of the glucose-fructose oxidoreductase gene was found to be improved due to greatly reduced production of xylitol, a detrimental by-product of xylose metabolism synthesized during fermentation.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {6}
}
Works referenced in this record:
Nuclear Magnetic Resonance Studies of Acetic Acid Inhibition of Rec Zymomonas mobilis ZM4(pZB5)
book, January 2000
- Kim, In S.; Barrow, Kevin D.; Rogers, Peter L.
- Twenty-First Symposium on Biotechnology for Fuels and Chemicals
Sorbitol promotes growth of Zymomonas mobilis in environments with high concentrations of sugar: evidence for a physiological function of glucose-fructose oxidoreductase in osmoprotection.
journal, January 1994
- Loos, H.; Krämer, R.; Sahm, H.
- Journal of Bacteriology, Vol. 176, Issue 24
The Effect of Glucose on High-Level Xylose Fermentations by Recombinant Zymomonas in Batch and Fed-Batch Fermentations
book, January 1999
- Lawford, Hugh G.; Rousseau, Joyce D.
- Twentieth Symposium on Biotechnology for Fuels and Chemicals
d-Xylose (d-glucose) isomerase from Arthrobacter strain N.R.R.L. B3728. Purification and properties
journal, July 1991
- Smith, C. A.; Rangarajan, M.; Hartley, B. S.
- Biochemical Journal, Vol. 277, Issue 1
Ethanol production from cellulose by coupled saccharification/fermentation usingSaccharomyces cerevisiae and cellulase complex fromSclerotiun rolfsii UV-8 mutant
journal, September 1992
- Deshpande, Mukund V.
- Applied Biochemistry and Biotechnology, Vol. 36, Issue 3, p. 227-234
Site-specific and directional gene replacement mediated by Cre recombinase
journal, October 2000
- Trinh, K. Ryan; Morrison, Sherie L.
- Journal of Immunological Methods, Vol. 244, Issue 1-2
Microbial Cellulose Utilization: Fundamentals and Biotechnology
journal, September 2002
- Lynd, L. R.; Weimer, P. J.; van Zyl, W. H.
- Microbiology and Molecular Biology Reviews, Vol. 66, Issue 3, p. 506-577
Export of the periplasmic NADP-containing glucose-fructose oxidoreductase of Zymomonas mobilis
journal, July 1996
- Wiegert, Thomas; Sahm, Hermann; Sprenger, G. A.
- Archives of Microbiology, Vol. 166, Issue 1
Pentose metabolism in Zymomonas mobilis wild-type and recombinant strains
journal, December 1992
- Feldmann, SigrunD.; Sahm, Hermann; Sprenger, GeorgA.
- Applied Microbiology and Biotechnology, Vol. 38, Issue 3
Metabolic Engineering of a Pentose Metabolism Pathway in Ethanologenic Zymomonas mobilis
journal, January 1995
- Zhang, M.; Eddy, C.; Deanda, K.
- Science, Vol. 267, Issue 5195
Bacteriophage P1 cre gene and its regulatory region
journal, January 1986
- Sternberg, N.; Sauer, B.; Hoess, R.
- Journal of Molecular Biology, Vol. 187, Issue 2
Rapid ethanol production from sucrose without by-product formation
journal, September 1993
- Kirk, Lisa A.; Doelle, Horst W.
- Biotechnology Letters, Vol. 15, Issue 9
Cellular biosensing system for assessing immunomodulating effects on the inducible nitric oxide synthase (iNOS) cascade
journal, January 2003
- Kamei, Ken-ichiro; Haruyama, Tetsuya; Mie, Masayasu
- Biotechnology Letters, Vol. 25, Issue 4, p. 321-325
Bacteriophage P1 site-specific recombination
journal, August 1981
- Sternberg, Nat; Hamilton, Daniel
- Journal of Molecular Biology, Vol. 150, Issue 4
Glucose-fructose oxidoreductase, a new enzyme isolated from Zymomonas mobilis that is responsible for sorbitol production.
journal, January 1986
- Zachariou, M.; Scopes, R. K.
- Journal of Bacteriology, Vol. 167, Issue 3
Kinetic and Nuclear Magnetic Resonance Studies of Xylose Metabolism by Recombinant Zymomonas mobilisZM4(pZB5)
journal, January 2000
- Kim, In Seop; Barrow, Kevin D.; Rogers, Peter L.
- Applied and Environmental Microbiology, Vol. 66, Issue 1