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Title: Multi-stage microbial system for continuous hydrogen production

Abstract

A method of using sequential chemostat culture vessels to provide continuous H.sub.2 production, in which photosynthetic O.sub.2 evolution and H.sub.2 photoproduction are separated physically into two separate bioreactors, comprising: a) growing a microorganism culture able to continuously generate H.sub.2 by photosynthetically producing cells at about the early-to-late log state in a first photobioreactor operating as a sulfur chemostat under aerobic and/or conditions; b) continuously feeding cells from the first photobioreactor to a second photobioreactor operating under anaerobic conditions and sulfur deprivation conditions resulting from constant uptake of sulfate in the first bioreactor and a low rate of culture flow between the first and second bioreactors, for induction of hydrogenase and H.sub.2 photoproduction to allow for continuous cultivation of the microorganism's cells in the first photobioreactor and constant H.sub.2 production in the second photobioreactor, and c) H.sub.2 gas from the second photobioreactor.

Inventors:
; ;
Issue Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1176340
Patent Number(s):
7732174
Application Number:
10/543,866
Assignee:
Alliance For Sustainable Energy, LLC (Golden, CO)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C - CHEMISTRY C12 - BIOCHEMISTRY C12M - APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
DOE Contract Number:  
AC36-99GO10337
Resource Type:
Patent
Resource Relation:
Patent File Date: 2003 Oct 01
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 08 HYDROGEN

Citation Formats

Kosourov, Sergey, Ghirardi, Maria L., and Seibert, Michael. Multi-stage microbial system for continuous hydrogen production. United States: N. p., 2010. Web.
Kosourov, Sergey, Ghirardi, Maria L., & Seibert, Michael. Multi-stage microbial system for continuous hydrogen production. United States.
Kosourov, Sergey, Ghirardi, Maria L., and Seibert, Michael. Tue . "Multi-stage microbial system for continuous hydrogen production". United States. https://www.osti.gov/servlets/purl/1176340.
@article{osti_1176340,
title = {Multi-stage microbial system for continuous hydrogen production},
author = {Kosourov, Sergey and Ghirardi, Maria L. and Seibert, Michael},
abstractNote = {A method of using sequential chemostat culture vessels to provide continuous H.sub.2 production, in which photosynthetic O.sub.2 evolution and H.sub.2 photoproduction are separated physically into two separate bioreactors, comprising: a) growing a microorganism culture able to continuously generate H.sub.2 by photosynthetically producing cells at about the early-to-late log state in a first photobioreactor operating as a sulfur chemostat under aerobic and/or conditions; b) continuously feeding cells from the first photobioreactor to a second photobioreactor operating under anaerobic conditions and sulfur deprivation conditions resulting from constant uptake of sulfate in the first bioreactor and a low rate of culture flow between the first and second bioreactors, for induction of hydrogenase and H.sub.2 photoproduction to allow for continuous cultivation of the microorganism's cells in the first photobioreactor and constant H.sub.2 production in the second photobioreactor, and c) H.sub.2 gas from the second photobioreactor.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {6}
}

Works referenced in this record:

Sustained hydrogen photoproduction byChlamydomonas reinhardtii: Effects of culture parameters
journal, May 2002


Probing green algal hydrogen production
journal, October 2002