skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Computational method and system for modeling, analyzing, and optimizing DNA amplification and synthesis

Abstract

A computational method and computer-based system of modeling DNA synthesis for the design and interpretation of PCR amplification, parallel DNA synthesis, and microarray chip analysis. The method and system include modules that address the bioinformatics, kinetics, and thermodynamics of DNA amplification and synthesis. Specifically, the steps of DNA selection, as well as the kinetics and thermodynamics of DNA hybridization and extensions, are addressed, which enable the optimization of the processing and the prediction of the products as a function of DNA sequence, mixing protocol, time, temperature and concentration of species.

Inventors:
; ;
Issue Date:
Research Org.:
Lawrence Livermore National Security, LLC, Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1176277
Patent Number(s):
7,711,491
Application Number:
10/840,779
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA)
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
97 MATHEMATICS AND COMPUTING; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Vandersall, Jennifer A., Gardner, Shea N., and Clague, David S. Computational method and system for modeling, analyzing, and optimizing DNA amplification and synthesis. United States: N. p., 2010. Web.
Vandersall, Jennifer A., Gardner, Shea N., & Clague, David S. Computational method and system for modeling, analyzing, and optimizing DNA amplification and synthesis. United States.
Vandersall, Jennifer A., Gardner, Shea N., and Clague, David S. Tue . "Computational method and system for modeling, analyzing, and optimizing DNA amplification and synthesis". United States. https://www.osti.gov/servlets/purl/1176277.
@article{osti_1176277,
title = {Computational method and system for modeling, analyzing, and optimizing DNA amplification and synthesis},
author = {Vandersall, Jennifer A. and Gardner, Shea N. and Clague, David S.},
abstractNote = {A computational method and computer-based system of modeling DNA synthesis for the design and interpretation of PCR amplification, parallel DNA synthesis, and microarray chip analysis. The method and system include modules that address the bioinformatics, kinetics, and thermodynamics of DNA amplification and synthesis. Specifically, the steps of DNA selection, as well as the kinetics and thermodynamics of DNA hybridization and extensions, are addressed, which enable the optimization of the processing and the prediction of the products as a function of DNA sequence, mixing protocol, time, temperature and concentration of species.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {5}
}

Patent:

Save / Share: