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Title: Flow cytometer acquisition and detection system

Abstract

A flow cytometer has a flow cell through which a sample flows and at least one laser emitting an excitation beam for illuminating a corresponding interrogation region in the flow cell. Scattered and fluorescence light from each interrogation region is collected by one or more input fibers for that region, and the input fiber(s) are fed to a dispersion module for that interrogation region that disperses the incoming light into different spectral regions. The dispersed light is conveyed, such as by a plurality of output fibers, to one or more photosensitive detectors. Thus, time multiplexed light signals may be delivered to a detector whereby several unique light signals can be measured by a single detector.

Inventors:
; ; ;
Issue Date:
Research Org.:
Advanced Cytometry Instrumentation Systems, Inc., Amherst, NY (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1176275
Patent Number(s):
7709821
Application Number:
11/380,434
Assignee:
Advanced Cytometry Instrumentation Systems, Inc. (Amherst, NY)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G - PHYSICS G01 - MEASURING G01J - MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRA-RED, VISIBLE OR ULTRA-VIOLET LIGHT
DOE Contract Number:  
FG02-01ER83134
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Casstevens, Martin K., Burzynski, Ryszard, Weibel, John, and Kachynski, Alexander. Flow cytometer acquisition and detection system. United States: N. p., 2010. Web.
Casstevens, Martin K., Burzynski, Ryszard, Weibel, John, & Kachynski, Alexander. Flow cytometer acquisition and detection system. United States.
Casstevens, Martin K., Burzynski, Ryszard, Weibel, John, and Kachynski, Alexander. Tue . "Flow cytometer acquisition and detection system". United States. https://www.osti.gov/servlets/purl/1176275.
@article{osti_1176275,
title = {Flow cytometer acquisition and detection system},
author = {Casstevens, Martin K. and Burzynski, Ryszard and Weibel, John and Kachynski, Alexander},
abstractNote = {A flow cytometer has a flow cell through which a sample flows and at least one laser emitting an excitation beam for illuminating a corresponding interrogation region in the flow cell. Scattered and fluorescence light from each interrogation region is collected by one or more input fibers for that region, and the input fiber(s) are fed to a dispersion module for that interrogation region that disperses the incoming light into different spectral regions. The dispersed light is conveyed, such as by a plurality of output fibers, to one or more photosensitive detectors. Thus, time multiplexed light signals may be delivered to a detector whereby several unique light signals can be measured by a single detector.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {5}
}

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