Metal resistant plants and phytoremediation of environmental contamination
Abstract
The present disclosure provides a method of producing transgenic plants which are resistant to at least one metal ion by transforming the plant with a recombinant DNA comprising a nucleic acid encoding a bacterial arsenic reductase under the control of a plant expressible promoter, and a nucleic acid encoding a nucleotide sequence encoding a phytochelatin biosynthetic enzyme under the control of a plant expressible promoter. The invention also relates a method of phytoremediation of a contaminated site by growing in the site a transgenic plant expressing a nucleic acid encoding a bacterial arsenate reductase and a nucleic acid encoding a phytochelatin biosynthetic enzyme.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of Georgia, Athens, GA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1176245
- Patent Number(s):
- 7700827
- Application Number:
- 10/450,731
- Assignee:
- The University of Georgia Research Foundation, Inc. (Athens, GA)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B09 - DISPOSAL OF SOLID WASTE B09C - RECLAMATION OF CONTAMINATED SOIL
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
- DOE Contract Number:
- FG07-96ER20257
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2001 Dec 13
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES
Citation Formats
Meagher, Richard B., Li, Yujing, and Dhankher, Om P. Metal resistant plants and phytoremediation of environmental contamination. United States: N. p., 2010.
Web.
Meagher, Richard B., Li, Yujing, & Dhankher, Om P. Metal resistant plants and phytoremediation of environmental contamination. United States.
Meagher, Richard B., Li, Yujing, and Dhankher, Om P. Tue .
"Metal resistant plants and phytoremediation of environmental contamination". United States. https://www.osti.gov/servlets/purl/1176245.
@article{osti_1176245,
title = {Metal resistant plants and phytoremediation of environmental contamination},
author = {Meagher, Richard B. and Li, Yujing and Dhankher, Om P.},
abstractNote = {The present disclosure provides a method of producing transgenic plants which are resistant to at least one metal ion by transforming the plant with a recombinant DNA comprising a nucleic acid encoding a bacterial arsenic reductase under the control of a plant expressible promoter, and a nucleic acid encoding a nucleotide sequence encoding a phytochelatin biosynthetic enzyme under the control of a plant expressible promoter. The invention also relates a method of phytoremediation of a contaminated site by growing in the site a transgenic plant expressing a nucleic acid encoding a bacterial arsenate reductase and a nucleic acid encoding a phytochelatin biosynthetic enzyme.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Apr 20 00:00:00 EDT 2010},
month = {Tue Apr 20 00:00:00 EDT 2010}
}
Works referenced in this record:
Tissue-specific expression of divergent actins in soybean root.
journal, April 1990
- McLean, B. G.; Eubanks, S.; Meagher, R. B.
- The Plant Cell, Vol. 2, Issue 4
Zinc and Cadmium Uptake by Hyperaccumulator Thlaspi caerulescens Grown in Nutrient Solution
journal, January 1995
- Brown, S. L.; Chaney, R. L.; Angle, J. S.
- Soil Science Society of America Journal, Vol. 59, Issue 1
Sequence of the fourth and fifth Photosystem II Type I chlorophyll a/b-binding protein genes of Arabidopsis thaliana and evidence for the presence of a full complement of the extended CAB gene family
journal, August 1992
- McGrath, J. Mitchell; Terzaghi, William B.; Sridhar, Padma
- Plant Molecular Biology, Vol. 19, Issue 5
The zntA gene of Escherichia coli encodes a Zn(II)-translocating P-type ATPase
journal, December 1997
- Rensing, C.; Mitra, B.; Rosen, B. P.
- Proceedings of the National Academy of Sciences, Vol. 94, Issue 26
Mercuric ion reduction and resistance in transgenic Arabidopsis thaliana plants expressing a modified bacterial merA gene.
journal, April 1996
- Rugh, C. L.; Wilde, H. D.; Stack, N. M.
- Proceedings of the National Academy of Sciences, Vol. 93, Issue 8
Regulation of S-Like Ribonuclease Levels in Arabidopsis. Antisense Inhibition of RNS1 or RNS2 Elevates Anthocyanin Accumulation
journal, January 1999
- Bariola, Pauline A.; MacIntosh, Gustavo C.; Green, Pamela J.
- Plant Physiology, Vol. 119, Issue 1
Spatial Proximity of Cys 113 , Cys 172 , and Cys 422 in the Metalloactivation Domain of the ArsA ATPase
journal, October 1996
- Bhattacharjee, Hiranmoy; Rosen, Barry P.
- Journal of Biological Chemistry, Vol. 271, Issue 40
Cryptic polyadenylation sites within the coding sequence of three yeast genes expressed in tobacco
journal, January 2000
- Grec, Sébastien; Wang, Yingchun; Le Guen, Laurence
- Gene, Vol. 242, Issue 1-2
Strong, constitutive expression of the Arabidopsis ACT2/ACT8 actin subclass in vegetative tissues
journal, July 1996
- An, Yong-Qiang; McDowell, John M.; Huang, Shurong
- The Plant Journal, Vol. 10, Issue 1
Both positive and negative regulatory elements mediate expression of a photoregulated CAB gene from Nicotiana plumbaginifolia.
journal, July 1988
- Castresana, C.; Garcia-Luque, I.; Alonso, E.
- The EMBO Journal, Vol. 7, Issue 7
Structural Analysis of Plant Genes
journal, June 1986
- Heidecker, G.; Messing, J.
- Annual Review of Plant Physiology, Vol. 37, Issue 1
The Potential for the Use of Metal-Accumulating Plants for the in Situ Decontamination of Metal-Polluted Soils
book, January 1993
- McGrath, S. P.; Sidoli, C. M. D.; Baker, A. J. M.
- Soil & Environment
RPS2 of Arabidopsis thaliana: a leucine-rich repeat class of plant disease resistance genes
journal, September 1994
- Bent, A.; Kunkel, B.; Dahlbeck, D.
- Science, Vol. 265, Issue 5180
Engineering tolerance and hyperaccumulation of arsenic in plants by combining arsenate reductase and γ-glutamylcysteine synthetase expression
journal, October 2002
- Dhankher, Om Parkash; Li, Yujing; Rosen, Barry P.
- Nature Biotechnology, Vol. 20, Issue 11
Bacterial resistance to heavy metals and metalloids
journal, August 1996
- Rosen, Barry P.
- JBIC Journal of Biological Inorganic Chemistry, Vol. 1, Issue 4
Zinc and Cadmium Uptake by Hyperaccumulator Thlaspi caerulescens and Metal Tolerant Silene vulgaris Grown on Sludge-Amended Soils
journal, June 1995
- Brown, Sally L.; Chaney, Rufus L.; Angle, J. Scott.
- Environmental Science & Technology, Vol. 29, Issue 6
Identification of an Essential Cysteinyl Residue in the ArsC Arsenate Reductase of Plasmid R773
journal, October 1995
- Liu, Jiyang; Gladysheva, Tatiana B.; Lee, Lana
- Biochemistry, Vol. 34, Issue 41
Vaccine engineering: enhancement of immunogenicity of synthetic peptide vaccines related to hepatitis in chemically defined models consisting of T- and B-cell epitopes.
journal, December 1989
- Tam, J. P.; Lu, Y. A.
- Proceedings of the National Academy of Sciences, Vol. 86, Issue 23
Arsenate reduction mediated by the plasmid-encoded ArsC protein is coupled to glutathione
journal, April 1994
- Oden, Kristine L.; Gladysheva, Tatiana B.; Rosen, Barry P.
- Molecular Microbiology, Vol. 12, Issue 2
Molecular analysis of an anion pump: Purification of the ArsC protein
journal, February 1991
- Rosen, Barry P.; Weigel, Ulrich; Monticello, Robert A.
- Archives of Biochemistry and Biophysics, Vol. 284, Issue 2
AtPCS1, a phytochelatin synthase from Arabidopsis: Isolation and in vitro reconstitution
journal, June 1999
- Vatamaniuk, O. K.; Mari, S.; Lu, Y. -P.
- Proceedings of the National Academy of Sciences, Vol. 96, Issue 12
Phytoremediation: A Novel Strategy for the Removal of Toxic Metals from the Environment Using Plants
journal, May 1995
- Salt, David E.; Blaylock, Michael; Kumar, Nanda P. B. A.
- Nature Biotechnology, Vol. 13, Issue 5
The reactivity of anti-peptide antibodies is a function of the atomic mobility of sites in a protein
journal, November 1984
- Tainer, John A.; Getzoff, Elizabeth D.; Alexander, Hannah
- Nature, Vol. 312, Issue 5990
Expression and regulation of the antimonite, arsenite, and arsenate resistance operon of Staphylococcus xylosus plasmid pSX267.
journal, January 1992
- Rosenstein, R.; Peschel, A.; Wieland, B.
- Journal of Bacteriology, Vol. 174, Issue 11
Overexpression of Glutathione Synthetase in Indian Mustard Enhances Cadmium Accumulation and Tolerance
journal, January 1999
- Liang Zhu, Yong; Pilon-Smits, Elizabeth A. H.; Jouanin, Lise
- Plant Physiology, Vol. 119, Issue 1
Development of transgenic yellow poplar for mercury phytoremediation
journal, October 1998
- Rugh, Clayton L.; Senecoff, Julie F.; Meagher, Richard B.
- Nature Biotechnology, Vol. 16, Issue 10
Phytodetoxification of hazardous organomercurials by genetically engineered plants
journal, February 2000
- Bizily, Scott P.; Rugh, Clayton L.; Meagher, Richard B.
- Nature Biotechnology, Vol. 18, Issue 2
Conserved expression of Arabidopsis thaliana poly (A) binding protein 2 (PAB2) in distinct vegetative and reproductive tissues
journal, May 2000
- Palanivelu, Ravishankar; Belostotsky, Dmitry A.; Meagher, Richard B.
- The Plant Journal, Vol. 22, Issue 3
Comparison of the expression of two highly homologous members of the soybean ribulose-1,5-bisphosphate carboxylase small subunit gene family
journal, June 1990
- Shirley, B. W.; Ham, D. P.; Senecoff, J. F.
- Plant Molecular Biology, Vol. 14, Issue 6
5′ proximal sequences of a soybean ribulose-1, 5-bisphosphate carboxylase small subunit gene direct light and phytochrome controlled transcription
journal, January 1987
- Shirley, B. W.; Berry-Lowe, S. L.; Rogers, S. G.
- Nucleic Acids Research, Vol. 15, Issue 16
Mechanism of Heavy Metal Ion Activation of Phytochelatin (PC) Synthase: BLOCKED THIOLS ARE SUFFICIENT FOR PC SYNTHASE-CATALYZED TRANSPEPTIDATION OF GLUTATHIONE AND RELATED THIOL PEPTIDES
journal, May 2000
- Vatamaniuk, Olena K.; Mari, Stéphane; Lu, Yu-Ping
- Journal of Biological Chemistry, Vol. 275, Issue 40
Immunogenicity comparison of a multi-antigenic peptide bearing V3 sequences of the human immunodeficiency virus type 1 with TAB9 protein in mice
journal, May 2000
- Cruz, Luis J.; Quintana, Diogenes; Iglesias, Enrique
- Journal of Peptide Science, Vol. 6, Issue 5, p. 217-224
Pink water, green plants and pink elephants
journal, December 2001
- Meagher, Richard B.
- Nature Biotechnology, Vol. 19, Issue 12
Cloning and expression of R-factor mediated arsenate resistance in Escherichia coli
journal, January 1983
- Mobley, Harry L. T.; Chen, Chih-Ming; Silver, Simon
- Molecular and General Genetics MGG, Vol. 191, Issue 3
Plant genetic engineering may help with environmental cleanup.
journal, April 1996
- Raskin, I.
- Proceedings of the National Academy of Sciences, Vol. 93, Issue 8
Phytochelatin Synthase Genes from Arabidopsis and the Yeast Schizosaccharomyces pombe
journal, June 1999
- Ha, Suk-Bong; Smith, Aaron P.; Howden, Ross
- The Plant Cell, Vol. 11, Issue 6
Rapid Isolation of Monoclonal Antibodies. Monitoring Enzymes in the Phytochelatin Synthesis Pathway
journal, November 2001
- Li, Yujing; Kandasamy, Muthugapatti K.; Meagher, Richard B.
- Plant Physiology, Vol. 127, Issue 3
High level arsenite resistance in Leishmania tarentolae is mediated by an active extrusion system
journal, September 1994
- Dey, Saibal; Papadopoulou, Barbara; Haimeur, Anass
- Molecular and Biochemical Parasitology, Vol. 67, Issue 1
Actin-organelle interaction: Association with chloroplast inArabidopsis leaf mesophyll cells
journal, October 1999
- Kandasamy, Muthugapatti K.; Meagher, Richard B.
- Cell Motility and the Cytoskeleton, Vol. 44, Issue 2, p. 110-118
Cadmium Tolerance and Accumulation in Indian Mustard Is Enhanced by Overexpressing γ-Glutamylcysteine Synthetase
journal, December 1999
- Zhu, Yong Liang; Pilon-Smits, Elizabeth A. H.; Tarun, Alice S.
- Plant Physiology, Vol. 121, Issue 4
Properties of the Arsenate Reductase of Plasmid R773
journal, June 1994
- Gladysheva, Tatiana B.; Oden, Kristine L.; Rosen, Barry P.
- Biochemistry, Vol. 33, Issue 23
Phytoremediation of methylmercury pollution: merB expression in Arabidopsis thaliana confers resistance to organomercurials
journal, June 1999
- Bizily, S. P.; Rugh, C. L.; Summers, A. O.
- Proceedings of the National Academy of Sciences, Vol. 96, Issue 12
Characterization of the Expression of the Petunia Glycine-Rich Protein-1 Gene Product
journal, June 1990
- Condit, Carol M.; McLean, B. Gail; Meagher, Richard B.
- Plant Physiology, Vol. 93, Issue 2
Resistance Mechanisms to Arsenicals and Antimonials
journal, January 1995
- Rosen, Barry P.
- Journal of Basic and Clinical Physiology and Pharmacology, Vol. 6, Issue 3-4
The Chromosomal Arsenic Resistance Genes of Thiobacillus ferrooxidans Have an Unusual Arrangement and Confer Increased Arsenic and Antimony Resistance to Escherichia coli
journal, May 2000
- Butcher, B. G.; Deane, S. M.; Rawlings, D. E.
- Applied and Environmental Microbiology, Vol. 66, Issue 5
Phytochelatins and Their Roles in Heavy Metal Detoxification
journal, July 2000
- Cobbett, Christopher S.
- Plant Physiology, Vol. 123, Issue 3
The potential of the high-biomass nickel hyperaccumulator Berkheya coddii for phytoremediation and phytomining
journal, December 1997
- Robinson, B. H.; Brooks, R. R.; Howes, A. W.
- Journal of Geochemical Exploration, Vol. 60, Issue 2
Metalloid Resistance Mechanisms in Prokaryotes
journal, January 1998
- Xu, C.; Zhou, T.; Kuroda, M.
- Journal of Biochemistry, Vol. 123, Issue 1
Fortified Foods and Phytoremediation. Two Sides of the Same Coin: Fig. 1.
journal, January 2001
- Guerinot, Mary Lou; Salt, David E.
- Plant Physiology, Vol. 125, Issue 1
Tolerance to toxic metals by a gene family of phytochelatin synthases from plants and yeast
journal, June 1999
- Clemens, S.
- The EMBO Journal, Vol. 18, Issue 12
Phytoremediation of toxic elemental and organic pollutants
journal, April 2000
- Meagher, Richard B.
- Current Opinion in Plant Biology, Vol. 3, Issue 2
BACTERIAL HEAVY METAL RESISTANCE: New Surprises
journal, October 1996
- Silver, Simon; Phung, Le T.
- Annual Review of Microbiology, Vol. 50, Issue 1
The glutathione-deficient, cadmium-sensitive mutant, cad2-1, of Arabidopsis thaliana is deficient in gamma-glutamylcysteine synthetase
journal, October 1998
- Cobbett, Christopher S.; May, Mike J.; Howden, Ross
- The Plant Journal, Vol. 16, Issue 1
Families of arsenic transporters
journal, May 1999
- Rosen, Barry P.
- Trends in Microbiology, Vol. 7, Issue 5