Antibody profiling sensitivity through increased reporter antibody layering
Abstract
A method for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an embodiment of the invention, the analyte is a drug, such as marijuana, Cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method comprises attaching antigens to a surface of a solid support in a preselected pattern to form an array wherein locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to the antigens in the array to form immune complexes; washing away antibodies that do form immune complexes; and detecting the immune complexes, to form an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to the subject's identity.
- Inventors:
- Issue Date:
- Research Org.:
- Idaho National Laboratory (INL), Idaho Falls, ID (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1176231
- Patent Number(s):
- 7695919
- Application Number:
- 11/691,096
- Assignee:
- Battelle Energy Alliance, LLC (Idaho Falls, ID)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- DOE Contract Number:
- AC07-05ID14517
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 47 OTHER INSTRUMENTATION
Citation Formats
Apel, William A., and Thompson, Vicki S. Antibody profiling sensitivity through increased reporter antibody layering. United States: N. p., 2010.
Web.
Apel, William A., & Thompson, Vicki S. Antibody profiling sensitivity through increased reporter antibody layering. United States.
Apel, William A., and Thompson, Vicki S. Tue .
"Antibody profiling sensitivity through increased reporter antibody layering". United States. https://www.osti.gov/servlets/purl/1176231.
@article{osti_1176231,
title = {Antibody profiling sensitivity through increased reporter antibody layering},
author = {Apel, William A. and Thompson, Vicki S},
abstractNote = {A method for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an embodiment of the invention, the analyte is a drug, such as marijuana, Cocaine (crystalline tropane alkaloid), methamphetamine, methyltestosterone, or mesterolone. The method comprises attaching antigens to a surface of a solid support in a preselected pattern to form an array wherein locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to the antigens in the array to form immune complexes; washing away antibodies that do form immune complexes; and detecting the immune complexes, to form an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to the subject's identity.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Apr 13 00:00:00 EDT 2010},
month = {Tue Apr 13 00:00:00 EDT 2010}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Simple and Reliable Salivary Tests for HIV and Hepatitis A and B Virus Diagnosis and Surveillance
journal, September 1993
- Parry, John V.
- Annals of the New York Academy of Sciences, Vol. 694, Issue 1 Saliva as a D
Yeast RNA polymerase II genes: isolation with antibody probes
journal, November 1983
- Young, R.; Davis, R.
- Science, Vol. 222, Issue 4625
Peptides on phage: a vast library of peptides for identifying ligands
journal, August 1990
- Cwirla, S. E.; Peters, E. A.; Barrett, R. W.
- Proceedings of the National Academy of Sciences, Vol. 87, Issue 16, p. 6378-6382
Testing for drugs of abuse in saliva and sweat
journal, August 1998
- Kidwell, David A.; Holland, Janel C.; Athanaselis, Sotiris
- Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 713, Issue 1, p. 111-135
Athletic drug Testing
journal, April 1998
- Bowers, Larry D.
- Clinics in Sports Medicine, Vol. 17, Issue 2
Fiber-Optic Immunosensor for the Detection of Fumonisin B1
journal, April 1996
- Thompson, Vicki S.; Maragos, Chris M.
- Journal of Agricultural and Food Chemistry, Vol. 44, Issue 4, p. 1041-1046
Combinatorial Peptide Library Protocols
book, January 1998
- Cabilly, Shmuel
- Methods in Molecular Biology
Preliminary Investigations into Tris(2,2'-bipyridyl) Ruthenium (III) as a Chemiluminescent Reagent for the Detection of 3,6-Diacetylmorphine (Heroin) on Surfaces
journal, September 2007
- Agg, Kent M.; Barnett, Neil W.; Lewis, Simon W.
- Journal of Forensic Sciences, Vol. 52, Issue 5, p. 1111-1114
Difference gel electrophoresis. A single gel method for detecting changes in protein extracts
journal, January 1997
- Ünlü, Mustafa; Morgan, Mary E.; Minden, Jonathan S.
- Electrophoresis, Vol. 18, Issue 11, p. 2071-2077
Antibody profiling as an identification tool for forensic samples
conference, February 1999
- Thompson, Vicki S.; Barrett, Karen B.; Davis, Tilton
- Enabling Technologies for Law Enforcement and Security, vp.
Electrogenerated Chemiluminescence: An Oxidative-Reduction Type ECL Reaction Sequence Using Tripropyl Amine
journal, January 1990
- Leland, Jonathan K.; Powell, Michael J.
- Journal of The Electrochemical Society, Vol. 137, Issue 10, p. 3127-3131
Cloning of open reading frames and promoters from the Saccharomyces cerevisiae genome: construction of genomic libraries of random small fragments
journal, January 1986
- Santangelo, George M.; Tornow, Joanne; Moldave, Kivie
- Gene, Vol. 46, Issue 2-3, p. 181-186
Sensitivity of Two Enzyme-linked Immunosorbent Assay Tests in Relation to Western Blot in Detecting Human T-Cell Lymphotropic Virus Types I and II Infection among HIV-1 Infected Patients from São Paulo, Brazil
journal, March 1998
- Caterino-de-Araujo, Adele; de los Santos-Fortuna, Elizabeth; Meleiro, Mônica Cristina Zandoná
- Diagnostic Microbiology and Infectious Disease, Vol. 30, Issue 3, p. 173-182
Saliva Testing for Drugs of Abuse
journal, September 1993
- Cone, Edward J.
- Annals of the New York Academy of Sciences, Vol. 694, Issue 1, p. 91-127
Fluoroimmunoassays and immunofluorometric assays.
journal, March 1985
- Hemmilä, I.
- Clinical Chemistry, Vol. 31, Issue 3
Drugs of Abuse in Saliva: A Review
journal, January 1992
- Schramm, W.; Smith, R. H.; Craig, P. A.
- Journal of Analytical Toxicology, Vol. 16, Issue 1, p. 1-9
DNA SEQUENCING: Massively Parallel Genomics
journal, July 1997
- Fodor, S. P.
- Science, Vol. 277, Issue 5324
Exploring the Metabolic and Genetic Control of Gene Expression on a Genomic Scale
journal, October 1997
- DeRisi, J. L.; Iyer, Vishwanath R.; Brown, Patrick O.
- Science, Vol. 278, Issue 5338, p. 680-686
Random peptide libraries: a source of specific protein binding molecules
journal, July 1990
- Devlin, J.; Panganiban, L.; Devlin, P.
- Science, Vol. 249, Issue 4967
A new type of synthetic peptide library for identifying ligand-binding activity
journal, November 1991
- Lam, Kit S.; Salmon, Sydney E.; Hersh, Evan M.
- Nature, Vol. 354, Issue 6348
Searching for peptide ligands with an epitope library
journal, July 1990
- Scott, J.; Smith, G.
- Science, Vol. 249, Issue 4967
Antibody Fingerprinting: A Novel Method for Identifying Individual People and Animals
journal, July 1988
- Francoeur, Ann-Michele
- Nature Biotechnology, Vol. 6, Issue 7
Micromosaic Immunoassays
journal, November 2000
- Bernard, André; Michel, Bruno; Delamarche, Emmanuel
- Analytical Chemistry, Vol. 73, Issue 1, p. 8-12