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Title: Recombinant cells that highly express chromosomally-integrated heterologous gene

Abstract

Recombinant host cells are obtained that comprise (A) a heterologous, polypeptide-encoding polynucleotide segment, stably integrated into a chromosome, which is under transcriptional control of an endogenous promoter and (B) a mutation that effects increased expression of the heterologous segment, resulting in enhanced production by the host cells of each polypeptide encoded by that segment, relative to production of each polypeptide by the host cells in the absence of the mutation. The increased expression thus achieved is retained in the absence of conditions that select for cells displaying such increased expression. When the integrated segment comprises, for example, ethanol-production genes from an efficient ethanol producer like Zymomonas mobilis, recombinant Escherichia coli and other enteric bacterial cells within the present invention are capable of converting a wide range of biomass-derived sugars efficiently to ethanol.

Inventors:
; ;
Issue Date:
Research Org.:
Univ. of Florida, Gainesville, FL (United States)
Sponsoring Org.:
USDOE; US Dept. of Agriculture (USDA)
OSTI Identifier:
1176154
Patent Number(s):
7192772
Application Number:
09/620,060
Assignee:
The University of Florida Research Foundations, Inc. (Gainesville, FL)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
DOE Contract Number:  
FG05-86ER13574; 88-37233-3987
Resource Type:
Patent
Resource Relation:
Patent File Date: 2000 Jul 21
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS

Citation Formats

Ingram, Lonnie O., Ohta, Kazuyoshi, and Wood, Brent E. Recombinant cells that highly express chromosomally-integrated heterologous gene. United States: N. p., 2007. Web.
Ingram, Lonnie O., Ohta, Kazuyoshi, & Wood, Brent E. Recombinant cells that highly express chromosomally-integrated heterologous gene. United States.
Ingram, Lonnie O., Ohta, Kazuyoshi, and Wood, Brent E. Tue . "Recombinant cells that highly express chromosomally-integrated heterologous gene". United States. https://www.osti.gov/servlets/purl/1176154.
@article{osti_1176154,
title = {Recombinant cells that highly express chromosomally-integrated heterologous gene},
author = {Ingram, Lonnie O. and Ohta, Kazuyoshi and Wood, Brent E.},
abstractNote = {Recombinant host cells are obtained that comprise (A) a heterologous, polypeptide-encoding polynucleotide segment, stably integrated into a chromosome, which is under transcriptional control of an endogenous promoter and (B) a mutation that effects increased expression of the heterologous segment, resulting in enhanced production by the host cells of each polypeptide encoded by that segment, relative to production of each polypeptide by the host cells in the absence of the mutation. The increased expression thus achieved is retained in the absence of conditions that select for cells displaying such increased expression. When the integrated segment comprises, for example, ethanol-production genes from an efficient ethanol producer like Zymomonas mobilis, recombinant Escherichia coli and other enteric bacterial cells within the present invention are capable of converting a wide range of biomass-derived sugars efficiently to ethanol.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 20 00:00:00 EDT 2007},
month = {Tue Mar 20 00:00:00 EDT 2007}
}

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