skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Recombinant cells that highly express chromosomally-integrated heterologous gene

Abstract

Recombinant host cells are obtained that comprise (A) a heterologous, polypeptide-encoding polynucleotide segment, stably integrated into a chromosome, which is under transcriptional control of an endogenous promoter and (B) a mutation that effects increased expression of the heterologous segment, resulting in enhanced production by the host cells of each polypeptide encoded by that segment, relative to production of each polypeptide by the host cells in the absence of the mutation. The increased expression thus achieved is retained in the absence of conditions that select for cells displaying such increased expression. When the integrated segment comprises, for example, ethanol-production genes from an efficient ethanol producer like Zymomonas mobilis, recombinant Escherichia coli and other enteric bacterial cells within the present invention are capable of converting a wide range of biomass-derived sugars efficiently to ethanol.

Inventors:
; ;
Issue Date:
Research Org.:
Univ. of Florida, Gainesville, FL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1176154
Patent Number(s):
7192772
Application Number:
09/620,060
Assignee:
The University of Florida Research Foundations, Inc. (Gainesville, FL)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
DOE Contract Number:  
FG05-86ER3574
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS

Citation Formats

Ingram, Lonnie O., Ohta, Kazuyoshi, and Wood, Brent E.. Recombinant cells that highly express chromosomally-integrated heterologous gene. United States: N. p., 2007. Web.
Ingram, Lonnie O., Ohta, Kazuyoshi, & Wood, Brent E.. Recombinant cells that highly express chromosomally-integrated heterologous gene. United States.
Ingram, Lonnie O., Ohta, Kazuyoshi, and Wood, Brent E.. Tue . "Recombinant cells that highly express chromosomally-integrated heterologous gene". United States. https://www.osti.gov/servlets/purl/1176154.
@article{osti_1176154,
title = {Recombinant cells that highly express chromosomally-integrated heterologous gene},
author = {Ingram, Lonnie O. and Ohta, Kazuyoshi and Wood, Brent E.},
abstractNote = {Recombinant host cells are obtained that comprise (A) a heterologous, polypeptide-encoding polynucleotide segment, stably integrated into a chromosome, which is under transcriptional control of an endogenous promoter and (B) a mutation that effects increased expression of the heterologous segment, resulting in enhanced production by the host cells of each polypeptide encoded by that segment, relative to production of each polypeptide by the host cells in the absence of the mutation. The increased expression thus achieved is retained in the absence of conditions that select for cells displaying such increased expression. When the integrated segment comprises, for example, ethanol-production genes from an efficient ethanol producer like Zymomonas mobilis, recombinant Escherichia coli and other enteric bacterial cells within the present invention are capable of converting a wide range of biomass-derived sugars efficiently to ethanol.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2007},
month = {3}
}

Patent:

Save / Share:

Works referenced in this record:

A perspective on the biotechnological potential of extremophiles
journal, January 1992


Purification and characterization of phosphatidylethanolamine N-methyltransferase from Zymomonas mobilis.
journal, January 1987


Functional genes for cellobiose utilization in natural isolates of Escherichia coli.
journal, June 1987


Efficient ethanol production from glucose, lactose, and xylose by recombinant Escherichia coli.
journal, January 1989


Isolation, Crystallization and Properties of a New Cellulase of Clostridium thermocellum Overproduced in Escherichia coli
journal, October 1986


Expression and Secretion of a Cellulomonas fimi Exoglucanase in Saccharomyces cerevisiae
journal, January 1988


Induction of T4 DNA ligase in a recombinant strain ofEscherichia coli
journal, March 1989


Nucleotide sequence and deletion analysis of the xylanase gene (xynZ) of Clostridium thermocellum.
journal, October 1988


Cloning of the debranching-enzyme gene from Thermoanaerobium brockii into Escherichia coli and Bacillus subtilis.
journal, January 1987


Expression of theZymomonas mobilis alcohol dehydrogenase II (adhB) and pyruvate decarboxylase (pdc) genes inBacillus
journal, May 1994


Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas mobilis.
journal, June 1987


Genetic engineering of ethanol production in Escherichia coli.
journal, January 1987


Molecular cloning of Erwinia chrysanthemi pectinase and cellulase structural genes
journal, March 1985


Influence of ethanol on the hopanoid content and the fatty acid pattern in batch and continuous cultures of Zymomonas mobilis
journal, January 1985


Anaerobic regulation of pyruvate formate-lyase from Escherichia coli K-12.
journal, November 1988


Expression Vector for Zymomonas mobilis†
journal, January 1987


Fermentation of d-Xylose to Ethanol by Genetically Modified Klebsiella planticola
journal, January 1987


Ethanol production by recombinantescherichia coli carrying genes from zymomonas mobilis
journal, March 1991


Promoter and nucleotide sequences of the Zymomonas mobilis pyruvate decarboxylase.
journal, January 1987


Characterization of the total extractable lipids of Zymomonas mobilis var. mobilis
journal, October 1982


Establishment of a Host-vector System in Lactobacillus helveticus with β-Galactosidase Activity as a Selection Marker
journal, January 1992


Production of Ethanol from d-Xylose by Using d-Xylose Isomerase and Yeasts
journal, January 1981


Cloning and sequencing of the major intracellular serine protease gene of Bacillus subtilis.
journal, January 1986


Microbial tolerance to alcohols: role of the cell membrane
journal, February 1986


Transcriptional patterns for the thrS-infC-rplT operon of Escherichia coli
journal, March 1988


Pyruvate decarboxylase from Zymomonas mobilis. Isolation and partial characterization
journal, November 1986


Production of ethanol byClostridium thermosaccharolyticum: II. A quantitative model describing product distributions
journal, December 1989


Ethanol tolerance and carbohydrate metabolism in lactobacilli
journal, July 1992


Production of ethanol byClostridium thermosaccharolyticum: I. Effect of cell recycle and environmental parameters
journal, December 1989


Metabolic engineering of Klebsiella oxytoca M5A1 for ethanol production from xylose and glucose.
journal, January 1991


Cloning of ten distinct DNA fragments of Clostridium thermocellum coding for cellulases
journal, August 1985


Isolation of free ceramide from Zymomonas mobilis.
journal, January 1990


Lipid composition of Zymomonas mobilis: effects of ethanol and glucose.
journal, January 1983


Genetic engineering of soft-rot bacteria for ethanol production from lignocellulose
journal, May 1993


Thermal Tolerance of Zymomonas mobilis: Temperature-Induced Changes in Membrane Composition †
journal, January 1986


Bacterial sterol surrogates. Biosynthesis of the side-chain of bacteriohopanetetrol and of a carbocyclic pseudopentose from 13C-labelled glucose in Zymomonas mobilis
journal, January 1989


Differential effects of ethanol and hexanol on the Escherichia coli cell envelope.
journal, January 1980


Fermentation of d-Xylose and l-Arabinose to Ethanol by Erwinia chrysanthemi
journal, January 1987


Purification and properties of the endoglucanase C of Clostridium thermocellum produced in Escherichia coli
journal, May 1986


Cloning and expression of the structural gene for pyruvate decarboxylase of Zymomonas mobilis in Escherichia coli
journal, April 1986


Cellulose Fermentation by an Asporogenous Mutant and an Ethanol-Tolerant Mutant of Clostridium thermocellum
journal, January 1989


A Mutant of Escherichia Coli that Leaks Cellulase Activity Encoded by Cloned Cellulase Genes from Cellulomonas Fimi
journal, March 1984


Nucleotide sequence of the pyruvate decarboxylase gene from Zymomonas mobilis
journal, January 1987


Adaptation of membrane lipids to alcohols.
journal, January 1976


Energy and calcium ion dependence of proteolysis during sporulation of Bacillus subtilis cells.
journal, January 1990


Microbial xylanolytic systems
journal, November 1985


Alcohol production from glucose and xylose using Escherichia coli containing Zymomonas mobilis genes
journal, September 1988


Expression of a xylanase gene of Bacillus pumilus in Escherichia coli and Bacillus subtilis
journal, August 1985


Activation of an enhancerless gene by chromosomal integration.
journal, December 1986


Expression of the Escherichia coli xylose isomerase gene in Saccharomyces cerevisiae.
journal, January 1987


New method for generating deletions and gene replacements in Escherichia coli.
journal, January 1989


Characterization of Two Cel (Cellulose Degradation) Genes of Clostridium Thermocellum Coding for Endoglucanases
journal, September 1983