Title: Organo luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes

Abstract

A semiconductor nanocrystal compound is described capable of linking to an affinity molecule. The compound comprises (1) a semiconductor nanocrystal capable of emitting electromagnetic radiation and/or absorbing energy, and/or scattering or diffracting electromagnetic radiation--when excited by an electromagnetic radiation source or a particle beam; and (2) at least one linking agent, having a first portion linked to the semiconductor nanocrystal and a second portion capable of linking to an affinity molecule. The compound is linked to an affinity molecule to form a semiconductor nanocrystal probe capable of bonding with a detectable substance. subsequent exposure to excitation energy will excite the semiconductor nanocrystal in the probe causing the emission of electromagnetic radiation. Further described are processes for respectively: making the luminescent semiconductor nanocrystal compound; making the semiconductor nanocrystal probe; and using the probe to determine the presence of a detectable substance in a material.

Inventors:

Weiss, Shimon; Bruchez, Jr., Marcel; Alivisatos, Paul

Issue Date:

2006-09-05

Research Org.:

Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1175902

Patent Number(s):

7101718

Application Number:

10/918,622

Assignee:

The Regents of the University of California (Oakland, CA)

Patent Classifications (CPCs):

B - PERFORMING OPERATIONS B82 - NANOTECHNOLOGY B82Y - SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

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B - PERFORMING OPERATIONS B82 - NANOTECHNOLOGY B82Y - SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES

B82Y15/00 - Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/6816 - characterised by the detection means
C12Q2563/107 - fluorescence

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N33/533 - with fluorescent label
G01N33/542 - with steric inhibition or signal modification, e.g. fluorescent quenching
G01N33/54373 - {involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings}
G01N33/588 - {with semiconductor nanocrystal label, e.g. quantum dots}

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

Y10T428/2982 - Particulate matter [e.g., sphere, flake, etc.]
Y10T428/2989 - Microcapsule with solid core [includes liposome]
Y10T428/2991 - Coated
Y10T428/2993 - Silicic or refractory material containing [e.g., tungsten oxide, glass, cement, etc.]
Y10T428/2995 - Silane, siloxane or silicone coating
Y10T428/2996 - Glass particles or spheres
Y10T436/24 - Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

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DOE Contract Number:  

AC03-76SF00098

Resource Type:

Patent

Resource Relation:

Patent File Date: 2004 Aug 12

Country of Publication:

United States

Language:

English

Subject:

36 MATERIALS SCIENCE