Organo luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes
Abstract
A semiconductor nanocrystal compound is described capable of linking to an affinity molecule. The compound comprises (1) a semiconductor nanocrystal capable of emitting electromagnetic radiation and/or absorbing energy, and/or scattering or diffracting electromagnetic radiation--when excited by an electromagnetic radiation source or a particle beam; and (2) at least one linking agent, having a first portion linked to the semiconductor nanocrystal and a second portion capable of linking to an affinity molecule. The compound is linked to an affinity molecule to form a semiconductor nanocrystal probe capable of bonding with a detectable substance. subsequent exposure to excitation energy will excite the semiconductor nanocrystal in the probe causing the emission of electromagnetic radiation. Further described are processes for respectively: making the luminescent semiconductor nanocrystal compound; making the semiconductor nanocrystal probe; and using the probe to determine the presence of a detectable substance in a material.
- Inventors:
- Issue Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1175902
- Patent Number(s):
- 7101718
- Application Number:
- 10/918,622
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B82 - NANOTECHNOLOGY B82Y - SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- AC03-76SF00098
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2004 Aug 12
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE
Citation Formats
Weiss, Shimon, Bruchez, Jr., Marcel, and Alivisatos, Paul. Organo luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes. United States: N. p., 2006.
Web.
Weiss, Shimon, Bruchez, Jr., Marcel, & Alivisatos, Paul. Organo luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes. United States.
Weiss, Shimon, Bruchez, Jr., Marcel, and Alivisatos, Paul. Tue .
"Organo luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes". United States. https://www.osti.gov/servlets/purl/1175902.
@article{osti_1175902,
title = {Organo luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes},
author = {Weiss, Shimon and Bruchez, Jr., Marcel and Alivisatos, Paul},
abstractNote = {A semiconductor nanocrystal compound is described capable of linking to an affinity molecule. The compound comprises (1) a semiconductor nanocrystal capable of emitting electromagnetic radiation and/or absorbing energy, and/or scattering or diffracting electromagnetic radiation--when excited by an electromagnetic radiation source or a particle beam; and (2) at least one linking agent, having a first portion linked to the semiconductor nanocrystal and a second portion capable of linking to an affinity molecule. The compound is linked to an affinity molecule to form a semiconductor nanocrystal probe capable of bonding with a detectable substance. subsequent exposure to excitation energy will excite the semiconductor nanocrystal in the probe causing the emission of electromagnetic radiation. Further described are processes for respectively: making the luminescent semiconductor nanocrystal compound; making the semiconductor nanocrystal probe; and using the probe to determine the presence of a detectable substance in a material.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2006},
month = {9}
}
Works referenced in this record:
Characterization of quantum-confined CdS nanocrystallites stabilized by deoxyribonucleic acid (DNA)
journal, April 1992
- Coffer, J. L.; Bigham, S. R.; Pinizzotto, R. F.
- Nanotechnology, Vol. 3, Issue 2
Quantum Dot Bioconjugates for Ultrasensitive Nonisotopic Detection
journal, September 1998
- Chan, Warren C. W.; Nie, Shuming
- Science, Vol. 281, Issue 5385, p. 2016-2018
Quantum Dots as Inorganic DNA-Binding Proteins
journal, January 1996
- Murphy, Catherine J.; Brauns, Eric B.; Gearheart, Latha
- MRS Proceedings, Vol. 452
Semiconductor Nanocrystals as Fluorescent Biological Labels
patent, September 1998
- Bruchez Jr., Marcel; Moronne, Mario Moronne; Gin, Peter Gin
- Science, Vol. 281, Issue 5385, p. 2013-2016
Stabilization of CdS semiconductor nanoparticles against photodegradation by a silica coating procedure
journal, April 1998
- Correa-Duarte, Miguel A.; Giersig, Michael; Liz-Marzán, Luis M.
- Chemical Physics Letters, Vol. 286, Issue 5-6, p. 497-501
MICROSCOPY:Semiconductor Beacons Light Up Cell Structures
journal, September 1998
- Service, R. F.
- Science, Vol. 281, Issue 5385
Scintillation proximity assay: a versatile high-throughput screening technology
journal, July 1996
- Cook, Neil D.
- Drug Discovery Today, Vol. 1, Issue 7, p. 287-294
Multicolor Spectral Karyotyping of Human Chromosomes
journal, July 1996
- Schrock, E.; du Manoir, S.; Veldman, T.
- Science, Vol. 273, Issue 5274
Organization of 'nanocrystal molecules' using DNA
journal, August 1996
- Alivisatos, A. Paul; Johnsson, Kai P.; Peng, Xiaogang
- Nature, Vol. 382, Issue 6592
Synthesis of Nanosized Gold−Silica Core−Shell Particles
journal, January 1996
- Liz-Marzán, Luis M.; Giersig, Michael; Mulvaney, Paul
- Langmuir, Vol. 12, Issue 18
Epitaxial Growth of Highly Luminescent CdSe/CdS Core/Shell Nanocrystals with Photostability and Electronic Accessibility
journal, July 1997
- Peng, Xiaogang; Schlamp, Michael C.; Kadavanich, Andreas V.
- Journal of the American Chemical Society, Vol. 119, Issue 30, p. 7019-7029
Semiconductor Clusters, Nanocrystals, and Quantum Dots
journal, February 1996
- Alivisatos, A. P.
- Science, Vol. 271, Issue 5251, p. 933-937
Synthesis and Isolatin of a Homodimer of Cadmium Selenide Nanocrystals
journal, February 1997
- Peng, Xiaogang; Wilson, Troy E.; Alivisatos, A. Paul
- Angewandte Chemie International Edition in English, Vol. 36, Issue 12, p. 145-147
Protein-Sized Quantum Dot Luminescence Can Distinguish between "Straight", "Bent", and "Kinked" Oligonucleotides
journal, September 1995
- Mahtab, Rahina; Rogers, Jessica P.; Murphy, Catherine J.
- Journal of the American Chemical Society, Vol. 117, Issue 35
Perspectives on the Physical Chemistry of Semiconductor Nanocrystals
journal, January 1996
- Alivisatos, A. P.
- The Journal of Physical Chemistry, Vol. 100, Issue 31
(CdSe)ZnS Core−Shell Quantum Dots: Synthesis and Characterization of a Size Series of Highly Luminescent Nanocrystallites
journal, November 1997
- Dabbousi, B. O.; Rodriguez-Viejo, J.; Mikulec, F. V.
- The Journal of Physical Chemistry B, Vol. 101, Issue 46, p. 9463-9475
QUANTUM DOTS MEET BIOMOLECULES: Method uses brightly fluorescing semiconductor nanocrystals as labels
journal, September 1998
- Jacoby, Mitch
- Chemical & Engineering News, Vol. 76, Issue 39
Preparation and microscopic visualization of multicolor luminescent immunophosphors
journal, January 1992
- Beverloo, H. B.; van Schadewijk, A.; Bonnet, J.
- Cytometry, Vol. 13, Issue 6
Electronic Energy Transfer in CdSe Quantum Dot Solids
journal, February 1996
- Kagan, C. R.; Murray, C. B.; Nirmal, M.
- Physical Review Letters, Vol. 76, Issue 9
Preferential Adsorption of a “Kinked” DNA to a Neutral Curved Surface: Comparisons to and Implications for Nonspecific DNA−Protein Interactions
journal, January 1996
- Mahtab, Rahina; Rogers, Jessica P.; Singleton, Chainey P.
- Journal of the American Chemical Society, Vol. 118, Issue 30