skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Methods and compositions for efficient nucleic acid sequencing

Abstract

Disclosed are novel methods and compositions for rapid and highly efficient nucleic acid sequencing based upon hybridization with two sets of small oligonucleotide probes of known sequences. Extremely large nucleic acid molecules, including chromosomes and non-amplified RNA, may be sequenced without prior cloning or subcloning steps. The methods of the invention also solve various current problems associated with sequencing technology such as, for example, high noise to signal ratios and difficult discrimination, attaching many nucleic acid fragments to a surface, preparing many, longer or more complex probes and labelling more species.

Inventors:
Issue Date:
Research Org.:
The University Of Chicago, Chicago, IL (United States); Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1175815
Patent Number(s):
7070927
Application Number:
10/068,301
Assignee:
University of Chicago (Chicago, IL)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
W-31-109-ENG-38
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Drmanac, Radoje. Methods and compositions for efficient nucleic acid sequencing. United States: N. p., 2006. Web.
Drmanac, Radoje. Methods and compositions for efficient nucleic acid sequencing. United States.
Drmanac, Radoje. Tue . "Methods and compositions for efficient nucleic acid sequencing". United States. https://www.osti.gov/servlets/purl/1175815.
@article{osti_1175815,
title = {Methods and compositions for efficient nucleic acid sequencing},
author = {Drmanac, Radoje},
abstractNote = {Disclosed are novel methods and compositions for rapid and highly efficient nucleic acid sequencing based upon hybridization with two sets of small oligonucleotide probes of known sequences. Extremely large nucleic acid molecules, including chromosomes and non-amplified RNA, may be sequenced without prior cloning or subcloning steps. The methods of the invention also solve various current problems associated with sequencing technology such as, for example, high noise to signal ratios and difficult discrimination, attaching many nucleic acid fragments to a surface, preparing many, longer or more complex probes and labelling more species.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2006},
month = {7}
}

Patent:

Save / Share:

Works referenced in this record:

An Algorithm for the DNA Sequence Generation from k-Tuple Word Contents of the Minimal Number of Random Fragments
journal, April 1991


DNA sequencing by hybridization — a megasequencing method and a diagnostic tool?
journal, January 1994


Likelihood DNA Sequencing By Hybridization
journal, December 1993


Nucleic acid hybridization assays employing dA-tailed capture probes. Single capture methods
journal, June 1989


Light-directed, spatially addressable parallel chemical synthesis
journal, February 1991


Rapid shotgeun cloning utillizing the two base recongition endonuclease Cvi JI
journal, January 1992


Continuous, on-line DNA sequencing using oligodeoxynucleotide primers with multiple fluorophores.
journal, August 1988


Specificity of the nick-closing activity of bacteriophage T4 DNA ligase
journal, March 1989


Dissociation of Duplexes Formed by Hybridization of DNA with Gel-Immobilized Oligonucleotides
journal, February 1994


Site-directed modification of DNA duplexes by chemical ligation
journal, January 1988


A versatile solid support system for oligodeoxynucleotide probe-based hybridization assays
journal, January 1991


Multiplex DNA sequencing
journal, April 1988


Enhanced DNA sequencing by hybridization.
journal, April 1994


Light-generated oligonucleotide arrays for rapid DNA sequence analysis.
journal, May 1994


An oligonucleotide hybridization approach to DNA sequencing
journal, October 1989


Affinity chromatography of a sequence-specific DNA binding protein using teflon-linked oligonucleotides
journal, February 1988


Reliable Hybridization of Oligonucleotides as Short as Six Nucleotides
journal, September 1990


A new method for sequencing DNA.
journal, February 1977


1-Tuple DNA Sequencing: Computer Analysis
journal, August 1989


Covalent immobilization of DNA onto polystyrene microwells: The molecules are only bound at the 5′ end
journal, October 1991


DNA sequencing by hybridization: 100 bases read by a non-gel-based method.
journal, November 1991


DNA sequence determination by hybridization: a strategy for efficient large-scale sequencing
journal, June 1993


Genomic Southern analysis with alkaline-phosphatase-conjugated oligonucleotide probes and the chemiluminescent substrate AMPPD
journal, May 1991


DNA Sequence Confidence Estimation
journal, February 1994


Inhibitory Effects of Human and Bovine Milk Constituents on Rotavirus Infections
journal, December 2004


Quantification of picogram levels of specific DNA immobilized in microtiter wells
journal, April 1985


Technique for maintaining and screening many microbial cultures
journal, December 1989


Derivatization of unprotected polynucleotides
journal, January 1983


Genomic sequencing.
journal, April 1984


DNA sequencing by primer walking with strings of contiguous hexamers
journal, December 1992


Improved Chips for Sequencing by Hybridization
journal, October 1991


A method for DNA sequencing by hybridization with oligonucleotide matrix
journal, January 1991


Selecting varieties using a series of trials and a combinatorial ordering method
journal, January 1994


Constant denaturant capillary electrophoresis (CDCE): a high resolution approach to mutational anaylsis
journal, January 1994


Low pressure DNA shearing: a method for random DNA sequence analysis
journal, January 1990


One-step labelling of oligonucleotides with fluoresceine during automated synthesis
journal, January 1990


Convergent Evolution of Alternative Splices at Domain Boundaries of the BK Channel
journal, March 2009


Microarrays: their origins and applications
journal, June 1999


DNA sequencing with chain-terminating inhibitors
journal, December 1977


Microplate hybridization of amplified viral DNA segment.
journal, January 1990


Worldwide Distribution of a Common Methylenetetrahydrofolate Reductase Mutation
journal, May 1998


Sensitive detection of genes by sandwich hybridization and time-resolved fluorometry
journal, June 1987


A universal nucleoside for use at ambiguous sites in DNA primers
journal, June 1994


Fluorescent-labeled oligonucleotide probes: detection of hybrid formation in solution by fluorescence polarization spectroscopy
journal, January 1991


Report on the sequencing by Hybridization workshop
journal, August 1992


A novel method for nucleic acid sequence determination
journal, December 1988


A ligase-mediated gene detection technique
journal, August 1988


Sequencing by hybridization: Towards an automated sequencing of one million M13 clones arrayed on membranes
journal, January 1992


Labelling oligonucleotides to high specific activity (I)
journal, January 1989


Chemical ligation of DNA: the first non-enzymatic assembly of a biologically active gene
journal, January 1991