skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Double stranded nucleic acid biochips

Abstract

This invention describes a new method of constructing double-stranded DNA (dsDNA) microarrays based on the use of pre-synthesized or natural DNA duplexes without a stem-loop structure. The complementary oligonucleotide chains are bonded together by a novel connector that includes a linker for immobilization on a matrix. A non-enzymatic method for synthesizing double-stranded nucleic acids with this novel connector enables the construction of inexpensive and robust dsDNA/dsRNA microarrays. DNA-DNA and DNA-protein interactions are investigated using the microarrays.

Inventors:
;
Issue Date:
Research Org.:
The University Of Chicago, Chicago, IL (United States); Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1175749
Patent Number(s):
7049073
Application Number:
10/283,670
Assignee:
The University of Chicago (Chicago, IL)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07F - ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
DOE Contract Number:  
W-31-109-ENG-38
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Chernov, Boris, and Golova, Julia. Double stranded nucleic acid biochips. United States: N. p., 2006. Web.
Chernov, Boris, & Golova, Julia. Double stranded nucleic acid biochips. United States.
Chernov, Boris, and Golova, Julia. Tue . "Double stranded nucleic acid biochips". United States. https://www.osti.gov/servlets/purl/1175749.
@article{osti_1175749,
title = {Double stranded nucleic acid biochips},
author = {Chernov, Boris and Golova, Julia},
abstractNote = {This invention describes a new method of constructing double-stranded DNA (dsDNA) microarrays based on the use of pre-synthesized or natural DNA duplexes without a stem-loop structure. The complementary oligonucleotide chains are bonded together by a novel connector that includes a linker for immobilization on a matrix. A non-enzymatic method for synthesizing double-stranded nucleic acids with this novel connector enables the construction of inexpensive and robust dsDNA/dsRNA microarrays. DNA-DNA and DNA-protein interactions are investigated using the microarrays.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2006},
month = {5}
}

Patent:

Save / Share:

Works referenced in this record:

DNA-templated assembly and electrode attachment of a conducting silver wire
journal, February 1998


Parallel DNA: generation of a duplex between two Drosophila sequences in vitro
journal, November 1989


Solid-Phase PCR in Microwells: Effects of Linker Length and Composition on Tethering, Hybridization, and Extension
journal, February 2002


Parallel-Stranded DNA with Mixed AT/GC Composition:  Role of trans G·C Base Pairs in Sequence Dependent Helical Stability
journal, August 2000


DNA microarrays with stem–loop DNA probes: preparation and applications
journal, October 2001


Immobilization of oligodeoxyribonucleotides with multiple anchors to microchips
journal, February 2001


Double-stranded DNA arrays: next steps in the surface campaign
journal, June 1999


Quantifying DNA–protein interactions by double-stranded DNA arrays
journal, June 1999


A Multistep Chemical Modification Procedure To Create DNA Arrays on Gold Surfaces for the Study of Protein−DNA Interactions with Surface Plasmon Resonance Imaging
journal, September 1999


Electrochemistry at DNA-modified surfaces: new probes for charge transport through the double helix
journal, April 2001


Enzyme-Modulated Cleavage of dsDNA for Supramolecular Design of Biosensors
journal, November 2001


Morphology of 15-mer Duplexes Tethered to Au(111) Probed Using Scanning Probe Microscopy
journal, September 2001


Steric factors influencing hybridisation of nucleic acids to oligonucleotide arrays
journal, March 1997