Double stranded nucleic acid biochips
Abstract
This invention describes a new method of constructing double-stranded DNA (dsDNA) microarrays based on the use of pre-synthesized or natural DNA duplexes without a stem-loop structure. The complementary oligonucleotide chains are bonded together by a novel connector that includes a linker for immobilization on a matrix. A non-enzymatic method for synthesizing double-stranded nucleic acids with this novel connector enables the construction of inexpensive and robust dsDNA/dsRNA microarrays. DNA-DNA and DNA-protein interactions are investigated using the microarrays.
- Inventors:
- Issue Date:
- Research Org.:
- The University Of Chicago, Chicago, IL (United States); Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1175749
- Patent Number(s):
- 7049073
- Application Number:
- 10/283,670
- Assignee:
- The University of Chicago (Chicago, IL)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07F - ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS
- DOE Contract Number:
- W-31-109-ENG-38
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Chernov, Boris, and Golova, Julia. Double stranded nucleic acid biochips. United States: N. p., 2006.
Web.
Chernov, Boris, & Golova, Julia. Double stranded nucleic acid biochips. United States.
Chernov, Boris, and Golova, Julia. Tue .
"Double stranded nucleic acid biochips". United States. https://www.osti.gov/servlets/purl/1175749.
@article{osti_1175749,
title = {Double stranded nucleic acid biochips},
author = {Chernov, Boris and Golova, Julia},
abstractNote = {This invention describes a new method of constructing double-stranded DNA (dsDNA) microarrays based on the use of pre-synthesized or natural DNA duplexes without a stem-loop structure. The complementary oligonucleotide chains are bonded together by a novel connector that includes a linker for immobilization on a matrix. A non-enzymatic method for synthesizing double-stranded nucleic acids with this novel connector enables the construction of inexpensive and robust dsDNA/dsRNA microarrays. DNA-DNA and DNA-protein interactions are investigated using the microarrays.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2006},
month = {5}
}
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