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Title: Methods and compositions for simultaneous saccharification and fermentation

Abstract

The invention provides compositions and methods for the synergistic degradation of oligosaccharides by endoglucanases. The invention further provides recombinant host cells containing one or more genes encoding endoglucanses which are capable of the synergistic degradation of oligosaccharides. Preferred host cells of the invention are ethanologenic and capable of carrying out simultaneous saccharification and fermentation resulting in the production of ethanol from complex cellulose substrates.

Inventors:
;
Issue Date:
Research Org.:
Univ. of Florida, Gainesville, FL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1175699
Patent Number(s):
7026152
Application Number:
09/885,297
Assignee:
University of Florida Research Foundation, Inc. (Gainesville, FL)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
DOE Contract Number:  
FG02-96ER20222
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Ingram, Lonnie O'Neal, and Zhou, Shengde. Methods and compositions for simultaneous saccharification and fermentation. United States: N. p., 2006. Web.
Ingram, Lonnie O'Neal, & Zhou, Shengde. Methods and compositions for simultaneous saccharification and fermentation. United States.
Ingram, Lonnie O'Neal, and Zhou, Shengde. Tue . "Methods and compositions for simultaneous saccharification and fermentation". United States. https://www.osti.gov/servlets/purl/1175699.
@article{osti_1175699,
title = {Methods and compositions for simultaneous saccharification and fermentation},
author = {Ingram, Lonnie O'Neal and Zhou, Shengde},
abstractNote = {The invention provides compositions and methods for the synergistic degradation of oligosaccharides by endoglucanases. The invention further provides recombinant host cells containing one or more genes encoding endoglucanses which are capable of the synergistic degradation of oligosaccharides. Preferred host cells of the invention are ethanologenic and capable of carrying out simultaneous saccharification and fermentation resulting in the production of ethanol from complex cellulose substrates.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2006},
month = {4}
}

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Works referenced in this record:

Degradation of microcrystalline cellulose: Synergism between different endoglucanases ofCellulomonas sp. ATCC 21399
journal, January 1992


Conversion of Mixed Waste Office Paper to Ethanol by Genetically Engineered Klebsiella oxytoca Strain P2
journal, November 1995


An interlaboratory comparison of the performance of ethanol-producing micro-organisms in a xylose-rich acid hydroysate
journal, March 1994


Fuel Ethanol from Cellulosic Biomass
journal, March 1991


Parametric studies of ethanol production form xylose and other sugars by recombinantEscherichia coli
journal, July 1991


Purification and characterization of endoglucanase C from Clostridium cellulolyticum. Catalytic comparison with endoglucanase A
journal, October 1993


Cloned Erwinia chrysanthemi out genes enable Escherichia coli to selectively secrete a diverse family of heterologous proteins to its milieu.
journal, February 1991


Expression of theZymomonas mobilis alcohol dehydrogenase II (adhB) and pyruvate decarboxylase (pdc) genes inBacillus
journal, May 1994


Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas mobilis.
journal, June 1987


Genetic engineering of ethanol production in Escherichia coli.
journal, January 1987


Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans
journal, April 1979


Engineering endoglucanase-secreting strains of ethanologenic Klebsiella oxytoca P2
journal, June 1999


Enhancement of Expression and Apparent Secretion ofErwinia chrysanthemi Endoglucanase (Encoded bycelZ) in Escherichia coli B
journal, June 1999


Enteric Bacterial Catalysts for Fuel Ethanol Production
journal, October 1999


Extracellular Enzymes and Pathogenesis of Soft-Rot Erwinia
journal, September 1994


Fermentation of galacturonic acid and other sugars in orange peel hydrolysates by the ethanologenic strain of Escherichia coli
journal, March 1994


Ethanol production from AFEX pretreated corn fiber by recombinant bacteria
journal, August 1996


Ethanol production from hemicellulose hydrolysates of agricultural residues using genetically engineeredEscherichia coli strain KO11
journal, January 1996


Isolation and characterization of ethanol-tolerant mutants of Escherichia coli KO11 for fuel ethanol production
journal, February 1998


Synergism in cellulase systems
journal, January 1991


Fermentation of sweet whey by ethanologenicEscherichia coli
journal, June 1992


Methods for measuring cellulase activities
journal, January 1988


Detection system for Escherichia coli-specific virulence genes: absence of virulence determinants in B and C strains.
journal, January 1997


Characterization of a new endoglucanase from Erwinia chrysanthemi
journal, January 1987


The complete general secretory pathway in gram-negative bacteria.
journal, January 1993


Genetic engineering of soft-rot bacteria for ethanol production from lignocellulose
journal, May 1993


Mechanism of ethanol inhibition of fermentation in Zymomonas mobilis CP4.
journal, January 1985


Type III Protein Secretion Systems in Bacterial Pathogens of Animals and Plants
journal, June 1998


Simultaneous expression of genes encoding endoglucanase and ?-glucosidase in Zymomonas mobilis
journal, September 1993


Cloning of the Acetobacter xylinum cellulase gene and its expression in Escherichia coli and Zymomonas mobilis
journal, December 1994


Expression of a thermostable cellulase gene from a thermophilic anaerobe in Saccharomyces cerevisiae
journal, January 1990


Conversion of xylan to ethanol by ethanologenic strains of Escherichia coli and Klebsiella oxytoca.
journal, January 1992