skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Methods and compositions for simultaneous saccharification and fermentation

Abstract

The invention provides compositions and methods for the synergistic degradation of oligosaccharides by endoglucanases. The invention further provides recombinant host cells containing one or more genes encoding endoglucanses which are capable of the synergistic degradation of oligosaccharides. Preferred host cells of the invention are ethanologenic and capable of carrying out simultaneous saccharification and fermentation resulting in the production of ethanol from complex cellulose substrates.

Inventors:
;
Issue Date:
Research Org.:
University of Florida Research Foundation, Inc., Gainesville, FL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1175699
Patent Number(s):
7,026,152
Application Number:
09/885,297
Assignee:
University of Florida Research Foundation, Inc. (Gainesville, FL)
DOE Contract Number:  
FG02-96ER20222
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Ingram, Lonnie O'Neal, and Zhou, Shengde. Methods and compositions for simultaneous saccharification and fermentation. United States: N. p., 2006. Web.
Ingram, Lonnie O'Neal, & Zhou, Shengde. Methods and compositions for simultaneous saccharification and fermentation. United States.
Ingram, Lonnie O'Neal, and Zhou, Shengde. Tue . "Methods and compositions for simultaneous saccharification and fermentation". United States. https://www.osti.gov/servlets/purl/1175699.
@article{osti_1175699,
title = {Methods and compositions for simultaneous saccharification and fermentation},
author = {Ingram, Lonnie O'Neal and Zhou, Shengde},
abstractNote = {The invention provides compositions and methods for the synergistic degradation of oligosaccharides by endoglucanases. The invention further provides recombinant host cells containing one or more genes encoding endoglucanses which are capable of the synergistic degradation of oligosaccharides. Preferred host cells of the invention are ethanologenic and capable of carrying out simultaneous saccharification and fermentation resulting in the production of ethanol from complex cellulose substrates.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2006},
month = {4}
}

Patent:

Save / Share:

Works referenced in this record:

Ethanol production from starch by recombinantEscherichia coli containing integrated genes for ethanol production and plasmid genes for saccharification
journal, May 1992

  • Guimaraes, Walter V.; Ohta, Kazuyoshi; Burchhardt, Gerhard
  • Biotechnology Letters, Vol. 14, Issue 5
  • DOI: 10.1007/BF01021257

Degradation of microcrystalline cellulose: Synergism between different endoglucanases ofCellulomonas sp. ATCC 21399
journal, January 1992

  • Poulsen, O. M.; Petersen, L. W.
  • Biotechnology and Bioengineering, Vol. 39, Issue 1
  • DOI: 10.1002/bit.260390118

Expression of Different Levels of Ethanologenic Enzymes from Zymomonas mobilis in Recombinant Strains of Escherichia coli†
journal, January 1988


Conversion of Mixed Waste Office Paper to Ethanol by Genetically Engineered Klebsiella oxytoca Strain P2
journal, November 1995

  • Brooks, Todd A.; Ingram, L. O.
  • Biotechnology Progress, Vol. 11, Issue 6
  • DOI: 10.1021/bp00036a003

An interlaboratory comparison of the performance of ethanol-producing micro-organisms in a xylose-rich acid hydroysate
journal, March 1994

  • Hahn-H�gerdal, B.; Jeppsson, H.; Olsson, L.
  • Applied Microbiology and Biotechnology, Vol. 41, Issue 1
  • DOI: 10.1007/BF00166083

Fuel Ethanol from Cellulosic Biomass
journal, March 1991


Parametric studies of ethanol production form xylose and other sugars by recombinantEscherichia coli
journal, July 1991

  • Beall, David S.; Ohta, K.; Ingram, L. O.
  • Biotechnology and Bioengineering, Vol. 38, Issue 3
  • DOI: 10.1002/bit.260380311

Purification and characterization of endoglucanase C from Clostridium cellulolyticum. Catalytic comparison with endoglucanase A
journal, October 1993


Cloned Erwinia chrysanthemi out genes enable Escherichia coli to selectively secrete a diverse family of heterologous proteins to its milieu.
journal, February 1991

  • He, S. Y.; Lindeberg, M.; Chatterjee, A. K.
  • Proceedings of the National Academy of Sciences, Vol. 88, Issue 3
  • DOI: 10.1073/pnas.88.3.1079

Expression of theZymomonas mobilis alcohol dehydrogenase II (adhB) and pyruvate decarboxylase (pdc) genes inBacillus
journal, May 1994

  • Barbosa, Maria de F. S.; Ingram, L. O.
  • Current Microbiology, Vol. 28, Issue 5
  • DOI: 10.1007/BF01573206

Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas mobilis.
journal, June 1987


Genetic engineering of ethanol production in Escherichia coli.
journal, January 1987


Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans
journal, April 1979

  • Figurski, D. H.; Helinski, D. R.
  • Proceedings of the National Academy of Sciences, Vol. 76, Issue 4
  • DOI: 10.1073/pnas.76.4.1648

Saccharification and fermentation of Sugar Cane bagasse by Klebsiella oxytoca P2 containing chromosomally integrated genes encoding the Zymomonas mobilis ethanol pathway
journal, June 1994

  • Doran, Joy B.; Aldrich, H. C.; Ingram, L. O.
  • Biotechnology and Bioengineering, Vol. 44, Issue 2, p. 240-247
  • DOI: 10.1002/bit.260440213

Engineering endoglucanase-secreting strains of ethanologenic Klebsiella oxytoca P2
journal, June 1999

  • Zhou, S.; Ingram, L. O.
  • Journal of Industrial Microbiology and Biotechnology, Vol. 22, Issue 6
  • DOI: 10.1038/sj.jim.2900666

Enhancement of Expression and Apparent Secretion ofErwinia chrysanthemi Endoglucanase (Encoded bycelZ) in Escherichia coli B
journal, June 1999


Fermentation of crystalline cellulose to ethanol by Klebsiella oxytoca containing chromosomally integrated Zymomonas mobilis genes
journal, September 1993

  • Doran, Joy B.; Ingram, L. O.
  • Biotechnology Progress, Vol. 9, Issue 5
  • DOI: 10.1021/bp00023a013

Enteric Bacterial Catalysts for Fuel Ethanol Production
journal, October 1999

  • Ingram, L. O.; Aldrich, H. C.; Borges, A. C. C.
  • Biotechnology Progress, Vol. 15, Issue 5
  • DOI: 10.1021/bp9901062

Chromosomal Integration of Heterologous DNA in Escherichia coli with Precise Removal of Markers and Replicons Used during Construction
journal, November 1999


Extracellular Enzymes and Pathogenesis of Soft-Rot Erwinia
journal, September 1994


Fermentation of galacturonic acid and other sugars in orange peel hydrolysates by the ethanologenic strain of Escherichia coli
journal, March 1994

  • Grohmann, K.; Baldwin, E. A.; Buslig, B. S.
  • Biotechnology Letters, Vol. 16, Issue 3
  • DOI: 10.1007/BF00134626

Ethanol production from AFEX pretreated corn fiber by recombinant bacteria
journal, August 1996

  • Moniruzzaman, Mohammed; Dien, Bruce S.; Ferrer, Betzabe
  • Biotechnology Letters, Vol. 18, Issue 8
  • DOI: 10.1007/BF00154635

Ethanol production from hemicellulose hydrolysates of agricultural residues using genetically engineeredEscherichia coli strain KO11
journal, January 1996

  • Asghari, A.; Bothast, R. J.; Doran, J. B.
  • Journal of Industrial Microbiology, Vol. 16, Issue 1
  • DOI: 10.1007/BF01569920

Isolation and characterization of ethanol-tolerant mutants of Escherichia coli KO11 for fuel ethanol production
journal, February 1998

  • Yomano, L. P.; York, S. W.; Ingram, L. O.
  • Journal of Industrial Microbiology and Biotechnology, Vol. 20, Issue 2
  • DOI: 10.1038/sj.jim.2900496

Synergism in cellulase systems
journal, January 1991


Isolation of the gene encoding the major endoglucanase of Erwinia chrysanthemi. Homology between cel genes of two strains of Erwinia chrysanthemi
journal, May 1987


Fermentation of sweet whey by ethanologenicEscherichia coli
journal, June 1992

  • Guimaraes, Walter V.; Dudey, Gregory L.; Ingram, L. O.
  • Biotechnology and Bioengineering, Vol. 40, Issue 1
  • DOI: 10.1002/bit.260400107

Methods for measuring cellulase activities
journal, January 1988


Sequence analysis of the cellulase-encoding celY gene of Erwinia chrysanthemi: a possible case of interspecies gene transfer
journal, September 1991


Detection system for Escherichia coli-specific virulence genes: absence of virulence determinants in B and C strains.
journal, January 1997


Characterization of a new endoglucanase from Erwinia chrysanthemi
journal, January 1987


The complete general secretory pathway in gram-negative bacteria.
journal, January 1993


Genetic engineering of soft-rot bacteria for ethanol production from lignocellulose
journal, May 1993

  • Beall, D. S.; Ingram, L. O.
  • Journal of Industrial Microbiology, Vol. 11, Issue 3
  • DOI: 10.1007/BF01583716

Mechanism of ethanol inhibition of fermentation in Zymomonas mobilis CP4.
journal, January 1985


Type III Protein Secretion Systems in Bacterial Pathogens of Animals and Plants
journal, June 1998


Simultaneous expression of genes encoding endoglucanase and ?-glucosidase in Zymomonas mobilis
journal, September 1993

  • Su, P.; Liu, C. -Q; Lucas, R. J.
  • Biotechnology Letters, Vol. 15, Issue 9
  • DOI: 10.1007/BF00131768

Conversion of hydrolysates of corn cobs and hulls into ethanol by recombinantEscherichia coli B containing integrated genes for ethanol production
journal, September 1992

  • Belll, D. S.; Ingram, L. O.; Ben-Bassat, A.
  • Biotechnology Letters, Vol. 14, Issue 9
  • DOI: 10.1007/BF01029153

Cloning of the Acetobacter xylinum cellulase gene and its expression in Escherichia coli and Zymomonas mobilis
journal, December 1994

  • Okamoto, T.; Yamano, S.; Ikeaga, H.
  • Applied Microbiology and Biotechnology, Vol. 42, Issue 4
  • DOI: 10.1007/BF00173921

Expression of a thermostable cellulase gene from a thermophilic anaerobe in Saccharomyces cerevisiae
journal, January 1990

  • Saito, Takao; Suzuki, Tohru; Hayashi, Akihiro
  • Journal of Fermentation and Bioengineering, Vol. 69, Issue 5
  • DOI: 10.1016/0922-338X(90)90106-7

Conversion of xylan to ethanol by ethanologenic strains of Escherichia coli and Klebsiella oxytoca.
journal, January 1992


Synergistic interaction of the Clostridium stercorarium cellulases Avicelase I (CelZ) and Avicelase II (CelY) in the degradation of microcrystalline cellulose
journal, February 1997