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Title: Methods for identifying an essential gene in a prokaryotic microorganism

Abstract

Methods are provided for the rapid identification of essential or conditionally essential DNA segments in any species of haploid cell (one copy chromosome per cell) that is capable of being transformed by artificial means and is capable of undergoing DNA recombination. This system offers an enhanced means of identifying essential function genes in diploid pathogens, such as gram-negative and gram-positive bacteria.

Inventors:
Issue Date:
Research Org.:
California Institute of Technology (CalTech), Pasadena, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1175636
Patent Number(s):
6991900
Application Number:
09/896,509
Assignee:
California Institute of Technology (Pasadena, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG03-99ER62855
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Shizuya, Hiroaki. Methods for identifying an essential gene in a prokaryotic microorganism. United States: N. p., 2006. Web.
Shizuya, Hiroaki. Methods for identifying an essential gene in a prokaryotic microorganism. United States.
Shizuya, Hiroaki. Tue . "Methods for identifying an essential gene in a prokaryotic microorganism". United States. https://www.osti.gov/servlets/purl/1175636.
@article{osti_1175636,
title = {Methods for identifying an essential gene in a prokaryotic microorganism},
author = {Shizuya, Hiroaki},
abstractNote = {Methods are provided for the rapid identification of essential or conditionally essential DNA segments in any species of haploid cell (one copy chromosome per cell) that is capable of being transformed by artificial means and is capable of undergoing DNA recombination. This system offers an enhanced means of identifying essential function genes in diploid pathogens, such as gram-negative and gram-positive bacteria.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2006},
month = {1}
}

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Works referenced in this record:

Construction and Characterization of a Human Bacterial Artificial Chromosome Library
journal, June 1996


The development and applications of the bacterial artificial chromosome cloning system.
journal, January 2001