Method for metabolizing carbazole in petroleum
Abstract
A method for selective cleavage of C--N bonds genes that encode for at least one enzyme suitable for conversion of carbazole to 2-aminobiphenyl-2,3-diol are combined with a gene encoding an amidase suitable for selectively cleaving a C--N bond in 2-aminobiphenyl-2,3-diol, forming an operon that encodes for cleavage of both C--N bonds of said carbazole. The operon is inserted into a host culture which, in turn, is contacted with the carbazole, resulting in selective cleavage of both C--N bonds of the carbazole. Also disclosed is a new microorganism that expresses a carbazole degradation trait constitutively and a method for degrading carbazole employing this microorganism.
- Inventors:
- Issue Date:
- Research Org.:
- National Energy Technology Laboratory (NETL), Pittsburgh, PA, Morgantown, WV (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1175494
- Patent Number(s):
- 6943006
- Application Number:
- 10/658,691
- Assignee:
- Alps Electric Co., Ltd. (Tokyo, JP)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C10 - PETROLEUM, GAS OR COKE INDUSTRIES C10G - CRACKING HYDROCARBON OILS
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AC26-99BC15219
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 02 PETROLEUM
Citation Formats
Kayser, Kevin J., and Kilbane, II, John J. Method for metabolizing carbazole in petroleum. United States: N. p., 2005.
Web.
Kayser, Kevin J., & Kilbane, II, John J. Method for metabolizing carbazole in petroleum. United States.
Kayser, Kevin J., and Kilbane, II, John J. Tue .
"Method for metabolizing carbazole in petroleum". United States. https://www.osti.gov/servlets/purl/1175494.
@article{osti_1175494,
title = {Method for metabolizing carbazole in petroleum},
author = {Kayser, Kevin J. and Kilbane, II, John J.},
abstractNote = {A method for selective cleavage of C--N bonds genes that encode for at least one enzyme suitable for conversion of carbazole to 2-aminobiphenyl-2,3-diol are combined with a gene encoding an amidase suitable for selectively cleaving a C--N bond in 2-aminobiphenyl-2,3-diol, forming an operon that encodes for cleavage of both C--N bonds of said carbazole. The operon is inserted into a host culture which, in turn, is contacted with the carbazole, resulting in selective cleavage of both C--N bonds of the carbazole. Also disclosed is a new microorganism that expresses a carbazole degradation trait constitutively and a method for degrading carbazole employing this microorganism.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2005},
month = {9}
}
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