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Title: Method for metabolizing carbazole in petroleum

Abstract

A method for selective cleavage of C--N bonds genes that encode for at least one enzyme suitable for conversion of carbazole to 2-aminobiphenyl-2,3-diol are combined with a gene encoding an amidase suitable for selectively cleaving a C--N bond in 2-aminobiphenyl-2,3-diol, forming an operon that encodes for cleavage of both C--N bonds of said carbazole. The operon is inserted into a host culture which, in turn, is contacted with the carbazole, resulting in selective cleavage of both C--N bonds of the carbazole. Also disclosed is a new microorganism that expresses a carbazole degradation trait constitutively and a method for degrading carbazole employing this microorganism.

Inventors:
;
Issue Date:
Research Org.:
National Energy Technology Laboratory (NETL), Pittsburgh, PA, Morgantown, WV (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1175494
Patent Number(s):
6943006
Application Number:
10/658,691
Assignee:
Alps Electric Co., Ltd. (Tokyo, JP)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C - CHEMISTRY C10 - PETROLEUM, GAS OR COKE INDUSTRIES C10G - CRACKING HYDROCARBON OILS
DOE Contract Number:  
AC26-99BC15219
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
02 PETROLEUM

Citation Formats

Kayser, Kevin J., and Kilbane, II, John J.. Method for metabolizing carbazole in petroleum. United States: N. p., 2005. Web.
Kayser, Kevin J., & Kilbane, II, John J.. Method for metabolizing carbazole in petroleum. United States.
Kayser, Kevin J., and Kilbane, II, John J.. Tue . "Method for metabolizing carbazole in petroleum". United States. https://www.osti.gov/servlets/purl/1175494.
@article{osti_1175494,
title = {Method for metabolizing carbazole in petroleum},
author = {Kayser, Kevin J. and Kilbane, II, John J.},
abstractNote = {A method for selective cleavage of C--N bonds genes that encode for at least one enzyme suitable for conversion of carbazole to 2-aminobiphenyl-2,3-diol are combined with a gene encoding an amidase suitable for selectively cleaving a C--N bond in 2-aminobiphenyl-2,3-diol, forming an operon that encodes for cleavage of both C--N bonds of said carbazole. The operon is inserted into a host culture which, in turn, is contacted with the carbazole, resulting in selective cleavage of both C--N bonds of the carbazole. Also disclosed is a new microorganism that expresses a carbazole degradation trait constitutively and a method for degrading carbazole employing this microorganism.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2005},
month = {9}
}

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Works referenced in this record:

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