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Title: Particle sizer and DNA sequencer

Abstract

An electrophoretic device separates and detects particles such as DNA fragments, proteins, and the like. The device has a capillary which is coated with a coating with a low refractive index such as Teflon.RTM. AF. A sample of particles is fluorescently labeled and injected into the capillary. The capillary is filled with an electrolyte buffer solution. An electrical field is applied across the capillary causing the particles to migrate from a first end of the capillary to a second end of the capillary. A detector light beam is then scanned along the length of the capillary to detect the location of the separated particles. The device is amenable to a high throughput system by providing additional capillaries. The device can also be used to determine the actual size of the particles and for DNA sequencing.

Inventors:
;
Issue Date:
Research Org.:
Univ. of California, Oakland, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1175491
Patent Number(s):
6942773
Application Number:
09/771,277
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

Citation Formats

Olivares, Jose A., and Stark, Peter C. Particle sizer and DNA sequencer. United States: N. p., 2005. Web.
Olivares, Jose A., & Stark, Peter C. Particle sizer and DNA sequencer. United States.
Olivares, Jose A., and Stark, Peter C. Tue . "Particle sizer and DNA sequencer". United States. https://www.osti.gov/servlets/purl/1175491.
@article{osti_1175491,
title = {Particle sizer and DNA sequencer},
author = {Olivares, Jose A. and Stark, Peter C.},
abstractNote = {An electrophoretic device separates and detects particles such as DNA fragments, proteins, and the like. The device has a capillary which is coated with a coating with a low refractive index such as Teflon.RTM. AF. A sample of particles is fluorescently labeled and injected into the capillary. The capillary is filled with an electrolyte buffer solution. An electrical field is applied across the capillary causing the particles to migrate from a first end of the capillary to a second end of the capillary. A detector light beam is then scanned along the length of the capillary to detect the location of the separated particles. The device is amenable to a high throughput system by providing additional capillaries. The device can also be used to determine the actual size of the particles and for DNA sequencing.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2005},
month = {9}
}

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Works referenced in this record:

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journal, January 1996


Chemiluminescence detection with a liquid core waveguide
journal, October 1999


Multi-capillary optical waveguides for DNA sequencing
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Spatial-Scanning Laser Fluorescence Detection for Capillary Electrophoresis
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An electro-osmotic flow system with integrated planar optical waveguide sensing
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Kinetics of the Interaction between DNA and the Type IC Restriction Enzyme Eco R124II
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Laser-Induced Fluorescence Detection by Liquid Core Waveguiding Applied to DNA Sequencing by Capillary Electrophoresis
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Time- and Wavelength-Resolved Fluorescence Detection for Capillary Zone Electrophoresis with Axial Excitation
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Postelectrophoresis Capillary Scanning Method for DNA Sequencing
journal, January 1996


Luminescence Detection with a Liquid Core Waveguide
journal, April 1999