Integrated crystal mounting and alignment system for high-throughput biological crystallography
Abstract
A method and apparatus for the transportation, remote and unattended mounting, and visual alignment and monitoring of protein crystals for synchrotron generated x-ray diffraction analysis. The protein samples are maintained at liquid nitrogen temperatures at all times: during shipment, before mounting, mounting, alignment, data acquisition and following removal. The samples must additionally be stably aligned to within a few microns at a point in space. The ability to accurately perform these tasks remotely and automatically leads to a significant increase in sample throughput and reliability for high-volume protein characterization efforts. Since the protein samples are placed in a shipping-compatible layered stack of sample cassettes each holding many samples, a large number of samples can be shipped in a single cryogenic shipping container.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of California, Oakland, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1175427
- Patent Number(s):
- 6918698
- Application Number:
- 10/319,282
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
C - CHEMISTRY C30 - CRYSTAL GROWTH C30B - SINGLE-CRYSTAL-GROWTH
- DOE Contract Number:
- AC03-76SF00098
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 47 OTHER INSTRUMENTATION
Citation Formats
Nordmeyer, Robert A., Snell, Gyorgy P., Cornell, Earl W., Kolbe, William, Yegian, Derek, Earnest, Thomas N., Jaklevic, Joseph M., Cork, Carl W., Santarsiero, Bernard D., and Stevens, Raymond C. Integrated crystal mounting and alignment system for high-throughput biological crystallography. United States: N. p., 2005.
Web.
Nordmeyer, Robert A., Snell, Gyorgy P., Cornell, Earl W., Kolbe, William, Yegian, Derek, Earnest, Thomas N., Jaklevic, Joseph M., Cork, Carl W., Santarsiero, Bernard D., & Stevens, Raymond C. Integrated crystal mounting and alignment system for high-throughput biological crystallography. United States.
Nordmeyer, Robert A., Snell, Gyorgy P., Cornell, Earl W., Kolbe, William, Yegian, Derek, Earnest, Thomas N., Jaklevic, Joseph M., Cork, Carl W., Santarsiero, Bernard D., and Stevens, Raymond C. Tue .
"Integrated crystal mounting and alignment system for high-throughput biological crystallography". United States. https://www.osti.gov/servlets/purl/1175427.
@article{osti_1175427,
title = {Integrated crystal mounting and alignment system for high-throughput biological crystallography},
author = {Nordmeyer, Robert A. and Snell, Gyorgy P. and Cornell, Earl W. and Kolbe, William and Yegian, Derek and Earnest, Thomas N. and Jaklevic, Joseph M. and Cork, Carl W. and Santarsiero, Bernard D. and Stevens, Raymond C.},
abstractNote = {A method and apparatus for the transportation, remote and unattended mounting, and visual alignment and monitoring of protein crystals for synchrotron generated x-ray diffraction analysis. The protein samples are maintained at liquid nitrogen temperatures at all times: during shipment, before mounting, mounting, alignment, data acquisition and following removal. The samples must additionally be stably aligned to within a few microns at a point in space. The ability to accurately perform these tasks remotely and automatically leads to a significant increase in sample throughput and reliability for high-volume protein characterization efforts. Since the protein samples are placed in a shipping-compatible layered stack of sample cassettes each holding many samples, a large number of samples can be shipped in a single cryogenic shipping container.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2005},
month = {7}
}