DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Apparatus and methods for high resolution separation of sample components on microfabricated channel devices

Abstract

Sample component separation apparatus and methods are described. An exemplary sample component separation apparatus includes a separation channel having a turn portion configured to reduce band-broadening caused by passage of a sample through the turn portion. To reduce band broadening caused by passage of a sample through a turn portion, the turn portion may be constructed and arranged to have a sample transport characteristic that is different from the corresponding sample transport characteristic of a substantially straight portion of the separation channel. For example, the turn portion may be configured with an effective channel width that is smaller than the effective channel widths of the substantially straight portion of the separation channel. The actual channel width of the turn portion may be smaller than the channel widths of the substantially straight portion; the effective channel width of the turn portion may be reduced by placing one or more sample transport barriers or constrictions in the turn portion of the channel. Alternatively, the sample velocity through the turn portion may be controlled so as to reduce band broadening. For example, sample transport barriers may be disposed in the turn portion so that sample components of a given band travel through themore » turn portion at substantially the same effective rate, whereby the band orientation remains substantially aligned along radial directions characteristic of the turn portion. Other a sample transport characteristics, such as electrical resistance or fluid flow resistance, of the turn portion may be adapted to reduce band broadening caused by passage of the sample through the turn portion.« less

Inventors:
; ; ;
Issue Date:
Research Org.:
Univ. of California, Oakland, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1175416
Patent Number(s):
6913679
Application Number:
09/249,274
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
FG0391ER61125
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
42 ENGINEERING

Citation Formats

Mathies, Richard A., Paegel, Brian, Simpson, Peter C., and Hutt, Lester. Apparatus and methods for high resolution separation of sample components on microfabricated channel devices. United States: N. p., 2005. Web.
Mathies, Richard A., Paegel, Brian, Simpson, Peter C., & Hutt, Lester. Apparatus and methods for high resolution separation of sample components on microfabricated channel devices. United States.
Mathies, Richard A., Paegel, Brian, Simpson, Peter C., and Hutt, Lester. Tue . "Apparatus and methods for high resolution separation of sample components on microfabricated channel devices". United States. https://www.osti.gov/servlets/purl/1175416.
@article{osti_1175416,
title = {Apparatus and methods for high resolution separation of sample components on microfabricated channel devices},
author = {Mathies, Richard A. and Paegel, Brian and Simpson, Peter C. and Hutt, Lester},
abstractNote = {Sample component separation apparatus and methods are described. An exemplary sample component separation apparatus includes a separation channel having a turn portion configured to reduce band-broadening caused by passage of a sample through the turn portion. To reduce band broadening caused by passage of a sample through a turn portion, the turn portion may be constructed and arranged to have a sample transport characteristic that is different from the corresponding sample transport characteristic of a substantially straight portion of the separation channel. For example, the turn portion may be configured with an effective channel width that is smaller than the effective channel widths of the substantially straight portion of the separation channel. The actual channel width of the turn portion may be smaller than the channel widths of the substantially straight portion; the effective channel width of the turn portion may be reduced by placing one or more sample transport barriers or constrictions in the turn portion of the channel. Alternatively, the sample velocity through the turn portion may be controlled so as to reduce band broadening. For example, sample transport barriers may be disposed in the turn portion so that sample components of a given band travel through the turn portion at substantially the same effective rate, whereby the band orientation remains substantially aligned along radial directions characteristic of the turn portion. Other a sample transport characteristics, such as electrical resistance or fluid flow resistance, of the turn portion may be adapted to reduce band broadening caused by passage of the sample through the turn portion.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2005},
month = {7}
}

Works referenced in this record:

Fluorescence scanner employing a macro scanning objective
conference, March 1996


Fluorescence scanner employing a macro scanning objective
conference, March 1996


High-throughput genetic analysis using microfabricated 96-sample capillary array electrophoresis microplates
journal, March 1998


High-throughput genetic analysis using microfabricated 96-sample capillary array electrophoresis microplates
journal, March 1998


Microchip Separations of Neutral Species via Micellar Electrokinetic Capillary Chromatography
journal, November 1995


High-speed separations of DNA sequencing reactions by capillary electrophoresis
journal, May 1990


High-speed separations of DNA sequencing reactions by capillary electrophoresis
journal, May 1990


Energy-transfer fluorescent reagents for DNA analyses
journal, February 1997


Energy-transfer fluorescent reagents for DNA analyses
journal, February 1997


High-performance electrophoresis
journal, January 1985


High-performance electrophoresis
journal, January 1985


Ultra-High-Speed DNA Sequencing Using Capillary Electrophoresis Chips
journal, October 1995


Ultra-High-Speed DNA Sequencing Using Capillary Electrophoresis Chips
journal, October 1995


Functional Integration of PCR Amplification and Capillary Electrophoresis in a Microfabricated DNA Analysis Device
journal, January 1996


Functional Integration of PCR Amplification and Capillary Electrophoresis in a Microfabricated DNA Analysis Device
journal, January 1996


Multiple Sheath-Flow Gel Capillary-Array Electrophoresis for Multicolor Fluorescent DNA Detection
journal, April 1994


Multiple Sheath-Flow Gel Capillary-Array Electrophoresis for Multicolor Fluorescent DNA Detection
journal, April 1994


Capillary electrophoresis and sample injection systems integrated on a planar glass chip
journal, September 1992


Capillary electrophoresis and sample injection systems integrated on a planar glass chip
journal, September 1992


Separation and analysis of DNA sequence reaction products by capillary gel electrophoresis
journal, September 1990


Separation and analysis of DNA sequence reaction products by capillary gel electrophoresis
journal, September 1990


Accessing Genetic Information with High-Density DNA Arrays
journal, October 1996


Accessing Genetic Information with High-Density DNA Arrays
journal, October 1996


Microsatellite-based cancer detection using capillary array electrophoresis and energy-transfer fluorescent primers
journal, January 1997


Microsatellite-based cancer detection using capillary array electrophoresis and energy-transfer fluorescent primers
journal, January 1997


Glass chips for high-speed capillary electrophoresis separations with submicrometer plate heights
journal, October 1993


Glass chips for high-speed capillary electrophoresis separations with submicrometer plate heights
journal, October 1993


High-Speed Separation of Antisense Oligonucleotides on a Micromachined Capillary Electrophoresis Device
journal, September 1994


High-Speed Separation of Antisense Oligonucleotides on a Micromachined Capillary Electrophoresis Device
journal, September 1994


Ultra-high-speed DNA fragment separations using microfabricated capillary array electrophoresis chips.
journal, November 1994


Ultra-high-speed DNA fragment separations using microfabricated capillary array electrophoresis chips.
journal, November 1994


DNA Sequencing on Microfabricated Electrophoretic Devices
journal, June 1998


Transport, Manipulation, and Reaction of Biological Cells On-Chip Using Electrokinetic Effects
journal, April 1997


Transport, Manipulation, and Reaction of Biological Cells On-Chip Using Electrokinetic Effects
journal, April 1997


DNA sequencing using capillary array electrophoresis
journal, September 1992


DNA sequencing using capillary array electrophoresis
journal, September 1992


Liquid flow in microchannels: experimental observations and computational analyses of microfluidics effects
journal, May 2003


A simple salting out procedure for extracting DNA from human nucleated cells
journal, January 1988


A simple salting out procedure for extracting DNA from human nucleated cells
journal, January 1988


High Speed Automated DNA Sequencing in Ultrathin Slab Gels
journal, January 1992


High Speed Automated DNA Sequencing in Ultrathin Slab Gels
journal, January 1992


Dispersion Sources for Compact Geometries on Microchips
journal, September 1998


DNA sequencing using a four-color confocal fluorescence capillary array scanner
journal, January 1996


DNA sequencing using a four-color confocal fluorescence capillary array scanner
journal, January 1996


Planar glass chips for capillary electrophoresis: repetitive sample injection, quantitation, and separation efficiency
journal, May 1993


Capillary array electrophoresis using laser-excited confocal fluorescence detection
journal, April 1992


Capillary array electrophoresis using laser-excited confocal fluorescence detection
journal, April 1992


Effects of Injection Schemes and Column Geometry on the Performance of Microchip Electrophoresis Devices
journal, April 1994


Microchip Capillary Electrophoresis with an Integrated Postcolumn Reactor
journal, October 1994


Microchip Capillary Electrophoresis with an Integrated Postcolumn Reactor
journal, October 1994


Integrated Microdevice for DNA Restriction Fragment Analysis
journal, January 1996


Integrated Microdevice for DNA Restriction Fragment Analysis
journal, January 1996


Capillary gel electrophoresis for rapid, high resolution DNA sequencing
journal, January 1990


Capillary gel electrophoresis for rapid, high resolution DNA sequencing
journal, January 1990


High-Speed Parallel Separation of DNA Restriction Fragments Using Capillary Array Electrophoresis
journal, December 1993


High-Speed Parallel Separation of DNA Restriction Fragments Using Capillary Array Electrophoresis
journal, December 1993


Micromachining a Miniaturized Capillary Electrophoresis-Based Chemical Analysis System on a Chip
journal, August 1993


Micromachining a Miniaturized Capillary Electrophoresis-Based Chemical Analysis System on a Chip
journal, August 1993


Global prevalence of putative haemochromatosis mutations.
journal, April 1997


Global prevalence of putative haemochromatosis mutations.
journal, April 1997