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Title: Isolated yeast promoter sequence and a method of regulated heterologous expression

Abstract

The present invention provides the promoter clone discovery of a glucoamylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated glucoamylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.

Inventors:
; ; ;
Issue Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1175369
Patent Number(s):
6900305
Application Number:
09/921,944
Assignee:
Battelle Memorial Institute (Richland, WA)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
DOE Contract Number:  
AC06-76RL01830
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Gao, Johnway, Skeen, Rodney S., Hooker, Brian S., and Anderson, Daniel B. Isolated yeast promoter sequence and a method of regulated heterologous expression. United States: N. p., 2005. Web.
Gao, Johnway, Skeen, Rodney S., Hooker, Brian S., & Anderson, Daniel B. Isolated yeast promoter sequence and a method of regulated heterologous expression. United States.
Gao, Johnway, Skeen, Rodney S., Hooker, Brian S., and Anderson, Daniel B. Tue . "Isolated yeast promoter sequence and a method of regulated heterologous expression". United States. https://www.osti.gov/servlets/purl/1175369.
@article{osti_1175369,
title = {Isolated yeast promoter sequence and a method of regulated heterologous expression},
author = {Gao, Johnway and Skeen, Rodney S. and Hooker, Brian S. and Anderson, Daniel B.},
abstractNote = {The present invention provides the promoter clone discovery of a glucoamylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated glucoamylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 31 00:00:00 EDT 2005},
month = {Tue May 31 00:00:00 EDT 2005}
}

Works referenced in this record:

Cloning of the Schwanniomyces occidentalis glucoamylase gene (GAM1) and its expression in Saccharomyces cerevisiae
journal, October 1990


Replacement of a Metabolic Pathway for Large-Scale Production of Lactic Acid from Engineered Yeasts
journal, September 1999


Yeast Protocols
book, February 1996


Direct alcohol fermentation of starch by a derepressed mutant ofSchwanniomyces castellii
journal, January 1994


Recent Advances in the Expression of Foreign Genes in Pichia pastoris
journal, August 1993


ISOLATION AND CHARACTERISATION OF THE AMYLOTIC SYSTEM OF SCHWANNIOMYCES CASTELLII
journal, September 1984


Optimization of single-cell-protein production from cassava starch using Schwanniomyces castellii
journal, November 1995


Expression and regulation of glucoamylase from the yeast Schwanniomyces castellii.
journal, January 1990


GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
journal, December 1987


Production of Amylolytic Enzymes by Several Yeast Species
journal, September 1982


Kinetics of Growth and Amylase Production of Saccharomycopsis fibuligera on Potato Processing Wastewater †
journal, January 1980


New Amylolytic Yeast Strains for Starch and Dextrin Fermentation
journal, January 1988


Fermentation of starch to ethanol by a co-culture of Saccharomycopsis fibuligera and Saccharomyces cerevisiae
journal, May 1993


The Molecular Biology of Schwanniomyces occidentalis Klocker
journal, January 1999


Analysis of the alpha-amylase gene of Schwanniomyces occidentalis and the secretion of its gene product in transformants of different yeast genera
journal, October 1989