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Title: Nucleic acid detection assays

Abstract

The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

Inventors:
; ; ; ;
Issue Date:
Research Org.:
Third Wave Technologies, Inc., Madison, WI, (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1175307
Patent Number(s):
6875572
Application Number:
10/081,806
Assignee:
Third Wave Technologies, Inc. (Madison, WI)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG02-94ER81891
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Prudent, James R., Hall, Jeff G., Lyamichev, Victor I., Brow, Mary Ann, and Dahlberg, James E. Nucleic acid detection assays. United States: N. p., 2005. Web.
Prudent, James R., Hall, Jeff G., Lyamichev, Victor I., Brow, Mary Ann, & Dahlberg, James E. Nucleic acid detection assays. United States.
Prudent, James R., Hall, Jeff G., Lyamichev, Victor I., Brow, Mary Ann, and Dahlberg, James E. Tue . "Nucleic acid detection assays". United States. https://www.osti.gov/servlets/purl/1175307.
@article{osti_1175307,
title = {Nucleic acid detection assays},
author = {Prudent, James R. and Hall, Jeff G. and Lyamichev, Victor I. and Brow, Mary Ann and Dahlberg, James E.},
abstractNote = {The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2005},
month = {4}
}

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