System and method for introduction and stabilization of genes in Thermus sp.
Abstract
A method for introducing and stabilizing heterologous and recombinant genes in a thermophilic host in which a characteristic gene defining a detectable host characteristic is inactivated or deleted from the thermophilic host, resulting in a modified thermophilic host expressing an absence of the detectable host characteristic. A DNA fragment of interest is inserted into the modified thermophilic host together with an intact characteristic gene, whereby the detectable host characteristic is restored to the thermophilic host, thereby enabling detection and confirmation of successful transformation using plasmid vectors and integration of the DNA fragment into the chromosome of the thermophilic host.
- Inventors:
- Issue Date:
- Research Org.:
- Gas Technology Institute, Des Plaines, IL (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1175255
- Patent Number(s):
- 6861258
- Application Number:
- 10/085,388
- Assignee:
- Gas Technology Institute (Des Plaines, IL)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- FG02-97ER62464
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES
Citation Formats
Kayser, Kevin J., Park, Ho-Shin, and Kilbane, II, John J. System and method for introduction and stabilization of genes in Thermus sp.. United States: N. p., 2005.
Web.
Kayser, Kevin J., Park, Ho-Shin, & Kilbane, II, John J. System and method for introduction and stabilization of genes in Thermus sp.. United States.
Kayser, Kevin J., Park, Ho-Shin, and Kilbane, II, John J. Tue .
"System and method for introduction and stabilization of genes in Thermus sp.". United States. https://www.osti.gov/servlets/purl/1175255.
@article{osti_1175255,
title = {System and method for introduction and stabilization of genes in Thermus sp.},
author = {Kayser, Kevin J. and Park, Ho-Shin and Kilbane, II, John J.},
abstractNote = {A method for introducing and stabilizing heterologous and recombinant genes in a thermophilic host in which a characteristic gene defining a detectable host characteristic is inactivated or deleted from the thermophilic host, resulting in a modified thermophilic host expressing an absence of the detectable host characteristic. A DNA fragment of interest is inserted into the modified thermophilic host together with an intact characteristic gene, whereby the detectable host characteristic is restored to the thermophilic host, thereby enabling detection and confirmation of successful transformation using plasmid vectors and integration of the DNA fragment into the chromosome of the thermophilic host.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 01 00:00:00 EST 2005},
month = {Tue Mar 01 00:00:00 EST 2005}
}
Works referenced in this record:
New Host-Vector System for Thermus spp. Based on the Malate Dehydrogenase Gene
journal, March 2001
- Kayser, Kevin J.; Kilbane, John J.
- Journal of Bacteriology, Vol. 183, Issue 5
A new Thermus-Escherichia coli shuttle integration vector system.
journal, January 1997
- Tamakoshi, M.; Uchida, M.; Tanabe, K.
- Journal of bacteriology, Vol. 179, Issue 15
Directed genomic integration, gene replacement, and integrative gene expression in Streptococcus thermophilus.
journal, January 1993
- Mollet, B.; Knol, J.; Poolman, B.
- Journal of Bacteriology, Vol. 175, Issue 14