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Title: System and method for introduction and stabilization of genes in Thermus sp.

Abstract

A method for introducing and stabilizing heterologous and recombinant genes in a thermophilic host in which a characteristic gene defining a detectable host characteristic is inactivated or deleted from the thermophilic host, resulting in a modified thermophilic host expressing an absence of the detectable host characteristic. A DNA fragment of interest is inserted into the modified thermophilic host together with an intact characteristic gene, whereby the detectable host characteristic is restored to the thermophilic host, thereby enabling detection and confirmation of successful transformation using plasmid vectors and integration of the DNA fragment into the chromosome of the thermophilic host.

Inventors:
; ;
Issue Date:
Research Org.:
Gas Technology Institute, Des Plaines, IL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1175255
Patent Number(s):
6861258
Application Number:
10/085,388
Assignee:
Gas Technology Institute (Des Plaines, IL)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG02-97ER62464
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Kayser, Kevin J., Park, Ho-Shin, and Kilbane, II, John J.. System and method for introduction and stabilization of genes in Thermus sp.. United States: N. p., 2005. Web.
Kayser, Kevin J., Park, Ho-Shin, & Kilbane, II, John J.. System and method for introduction and stabilization of genes in Thermus sp.. United States.
Kayser, Kevin J., Park, Ho-Shin, and Kilbane, II, John J.. Tue . "System and method for introduction and stabilization of genes in Thermus sp.". United States. https://www.osti.gov/servlets/purl/1175255.
@article{osti_1175255,
title = {System and method for introduction and stabilization of genes in Thermus sp.},
author = {Kayser, Kevin J. and Park, Ho-Shin and Kilbane, II, John J.},
abstractNote = {A method for introducing and stabilizing heterologous and recombinant genes in a thermophilic host in which a characteristic gene defining a detectable host characteristic is inactivated or deleted from the thermophilic host, resulting in a modified thermophilic host expressing an absence of the detectable host characteristic. A DNA fragment of interest is inserted into the modified thermophilic host together with an intact characteristic gene, whereby the detectable host characteristic is restored to the thermophilic host, thereby enabling detection and confirmation of successful transformation using plasmid vectors and integration of the DNA fragment into the chromosome of the thermophilic host.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2005},
month = {3}
}

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Works referenced in this record:

New Host-Vector System for Thermus spp. Based on the Malate Dehydrogenase Gene
journal, March 2001


A new Thermus-Escherichia coli shuttle integration vector system.
journal, January 1997