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Title: Automated macromolecular crystallization screening

Abstract

An automated macromolecular crystallization screening system wherein a multiplicity of reagent mixes are produced. A multiplicity of analysis plates is produced utilizing the reagent mixes combined with a sample. The analysis plates are incubated to promote growth of crystals. Images of the crystals are made. The images are analyzed with regard to suitability of the crystals for analysis by x-ray crystallography. A design of reagent mixes is produced based upon the expected suitability of the crystals for analysis by x-ray crystallography. A second multiplicity of mixes of the reagent components is produced utilizing the design and a second multiplicity of reagent mixes is used for a second round of automated macromolecular crystallization screening. In one embodiment the multiplicity of reagent mixes are produced by a random selection of reagent components.

Inventors:
; ;
Issue Date:
Research Org.:
The Regents of the Univ. of California, Oakland, CA (United States); Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1175254
Patent Number(s):
6860940
Application Number:
10/253,130
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE

Citation Formats

Segelke, Brent W., Rupp, Bernhard, and Krupka, Heike I.. Automated macromolecular crystallization screening. United States: N. p., 2005. Web.
Segelke, Brent W., Rupp, Bernhard, & Krupka, Heike I.. Automated macromolecular crystallization screening. United States.
Segelke, Brent W., Rupp, Bernhard, and Krupka, Heike I.. Tue . "Automated macromolecular crystallization screening". United States. https://www.osti.gov/servlets/purl/1175254.
@article{osti_1175254,
title = {Automated macromolecular crystallization screening},
author = {Segelke, Brent W. and Rupp, Bernhard and Krupka, Heike I.},
abstractNote = {An automated macromolecular crystallization screening system wherein a multiplicity of reagent mixes are produced. A multiplicity of analysis plates is produced utilizing the reagent mixes combined with a sample. The analysis plates are incubated to promote growth of crystals. Images of the crystals are made. The images are analyzed with regard to suitability of the crystals for analysis by x-ray crystallography. A design of reagent mixes is produced based upon the expected suitability of the crystals for analysis by x-ray crystallography. A second multiplicity of mixes of the reagent components is produced utilizing the design and a second multiplicity of reagent mixes is used for a second round of automated macromolecular crystallization screening. In one embodiment the multiplicity of reagent mixes are produced by a random selection of reagent components.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2005},
month = {3}
}

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Works referenced in this record:

Efficiency analysis of sampling protocols used in protein crystallization screening
journal, November 2001


XAct : a program for construction, automated setup and bookkeeping of crystallization experiments
journal, October 1999


Automatic preparation of protein crystals using laboratory robotics and automated visual inspection
journal, July 1988