Automated macromolecular crystallization screening
Abstract
An automated macromolecular crystallization screening system wherein a multiplicity of reagent mixes are produced. A multiplicity of analysis plates is produced utilizing the reagent mixes combined with a sample. The analysis plates are incubated to promote growth of crystals. Images of the crystals are made. The images are analyzed with regard to suitability of the crystals for analysis by x-ray crystallography. A design of reagent mixes is produced based upon the expected suitability of the crystals for analysis by x-ray crystallography. A second multiplicity of mixes of the reagent components is produced utilizing the design and a second multiplicity of reagent mixes is used for a second round of automated macromolecular crystallization screening. In one embodiment the multiplicity of reagent mixes are produced by a random selection of reagent components.
- Inventors:
- Issue Date:
- Research Org.:
- The Regents of the Univ. of California, Oakland, CA (United States); Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1175254
- Patent Number(s):
- 6860940
- Application Number:
- 10/253,130
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE
Citation Formats
Segelke, Brent W., Rupp, Bernhard, and Krupka, Heike I. Automated macromolecular crystallization screening. United States: N. p., 2005.
Web.
Segelke, Brent W., Rupp, Bernhard, & Krupka, Heike I. Automated macromolecular crystallization screening. United States.
Segelke, Brent W., Rupp, Bernhard, and Krupka, Heike I. Tue .
"Automated macromolecular crystallization screening". United States. https://www.osti.gov/servlets/purl/1175254.
@article{osti_1175254,
title = {Automated macromolecular crystallization screening},
author = {Segelke, Brent W. and Rupp, Bernhard and Krupka, Heike I.},
abstractNote = {An automated macromolecular crystallization screening system wherein a multiplicity of reagent mixes are produced. A multiplicity of analysis plates is produced utilizing the reagent mixes combined with a sample. The analysis plates are incubated to promote growth of crystals. Images of the crystals are made. The images are analyzed with regard to suitability of the crystals for analysis by x-ray crystallography. A design of reagent mixes is produced based upon the expected suitability of the crystals for analysis by x-ray crystallography. A second multiplicity of mixes of the reagent components is produced utilizing the design and a second multiplicity of reagent mixes is used for a second round of automated macromolecular crystallization screening. In one embodiment the multiplicity of reagent mixes are produced by a random selection of reagent components.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2005},
month = {3}
}
Works referenced in this record:
Efficiency analysis of sampling protocols used in protein crystallization screening
journal, November 2001
- Segelke, Brent W.
- Journal of Crystal Growth, Vol. 232, Issue 1-4
XAct : a program for construction, automated setup and bookkeeping of crystallization experiments
journal, October 1999
- Brodersen, D. E.; Jenner, L. B.; Andersen, G. R.
- Journal of Applied Crystallography, Vol. 32, Issue 5
Automatic preparation of protein crystals using laboratory robotics and automated visual inspection
journal, July 1988
- Ward, Keith B.; Perozzo, Mary Ann; Zuk, William M.
- Journal of Crystal Growth, Vol. 90, Issue 1-3