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Title: Universal light-switchable gene promoter system

Abstract

An artificial promoter system that can be fused upstream of any desired gene enabling reversible induction or repression of the expression of the gene at will in any suitable host cell or organisms by light is described. The design of the system is such that a molecule of the plant photoreceptor phytochrome is targeted to the specific DNA binding site in the promoter by a protein domain that is fused to the phytochrome and that specifically recognizes this binding site. This bound phytochrome, upon activation by light, recruits a second fusion protein consisting of a protein that binds to phytochrome only upon light activation and a transcriptional activation domain that activates expression of the gene downstream of the promoter.

Inventors:
; ; ;
Issue Date:
Research Org.:
Univ. of California, Oakland, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1175244
Patent Number(s):
6858429
Application Number:
10/227,035
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG03-87ER13742
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Quail, Peter H., Huq, Enamul, Tepperman, James, and Sato, Sae. Universal light-switchable gene promoter system. United States: N. p., 2005. Web.
Quail, Peter H., Huq, Enamul, Tepperman, James, & Sato, Sae. Universal light-switchable gene promoter system. United States.
Quail, Peter H., Huq, Enamul, Tepperman, James, and Sato, Sae. Tue . "Universal light-switchable gene promoter system". United States. https://www.osti.gov/servlets/purl/1175244.
@article{osti_1175244,
title = {Universal light-switchable gene promoter system},
author = {Quail, Peter H. and Huq, Enamul and Tepperman, James and Sato, Sae},
abstractNote = {An artificial promoter system that can be fused upstream of any desired gene enabling reversible induction or repression of the expression of the gene at will in any suitable host cell or organisms by light is described. The design of the system is such that a molecule of the plant photoreceptor phytochrome is targeted to the specific DNA binding site in the promoter by a protein domain that is fused to the phytochrome and that specifically recognizes this binding site. This bound phytochrome, upon activation by light, recruits a second fusion protein consisting of a protein that binds to phytochrome only upon light activation and a transcriptional activation domain that activates expression of the gene downstream of the promoter.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2005},
month = {2}
}

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Works referenced in this record:

Photoreceptors and Regulation of Flowering Time
journal, May 2000


Interactions Between Gravitropism and Phototropism in Plants
journal, June 2002


Binding of phytochrome B to its nuclear signalling partner PIF3 is reversibly induced by light
journal, August 1999


All in good time: the Arabidopsis circadian clock
journal, December 2000


Phytochrome B binds with greater apparent affinity than phytochrome A to the basic helix-loop-helix factor PIF3 in a reaction requiring the PAS domain of PIF3
journal, November 2000


Phytochromes, pif3 and light signalling go nuclear
journal, April 1999


Clock proteins: Turned over after hours?
journal, July 2000


Phytochrome photosensory signalling networks
journal, February 2002


Phytochrome-interacting factors
journal, December 2000


Light-dependent Translocation of a Phytochrome B-GFP Fusion Protein to the Nucleus in Transgenic Arabidopsis
journal, May 1999