Universal light-switchable gene promoter system
Abstract
An artificial promoter system that can be fused upstream of any desired gene enabling reversible induction or repression of the expression of the gene at will in any suitable host cell or organisms by light is described. The design of the system is such that a molecule of the plant photoreceptor phytochrome is targeted to the specific DNA binding site in the promoter by a protein domain that is fused to the phytochrome and that specifically recognizes this binding site. This bound phytochrome, upon activation by light, recruits a second fusion protein consisting of a protein that binds to phytochrome only upon light activation and a transcriptional activation domain that activates expression of the gene downstream of the promoter.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of California, Oakland, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1175244
- Patent Number(s):
- 6858429
- Application Number:
- 10/227,035
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FG03-87ER13742
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES
Citation Formats
Quail, Peter H., Huq, Enamul, Tepperman, James, and Sato, Sae. Universal light-switchable gene promoter system. United States: N. p., 2005.
Web.
Quail, Peter H., Huq, Enamul, Tepperman, James, & Sato, Sae. Universal light-switchable gene promoter system. United States.
Quail, Peter H., Huq, Enamul, Tepperman, James, and Sato, Sae. Tue .
"Universal light-switchable gene promoter system". United States. https://www.osti.gov/servlets/purl/1175244.
@article{osti_1175244,
title = {Universal light-switchable gene promoter system},
author = {Quail, Peter H. and Huq, Enamul and Tepperman, James and Sato, Sae},
abstractNote = {An artificial promoter system that can be fused upstream of any desired gene enabling reversible induction or repression of the expression of the gene at will in any suitable host cell or organisms by light is described. The design of the system is such that a molecule of the plant photoreceptor phytochrome is targeted to the specific DNA binding site in the promoter by a protein domain that is fused to the phytochrome and that specifically recognizes this binding site. This bound phytochrome, upon activation by light, recruits a second fusion protein consisting of a protein that binds to phytochrome only upon light activation and a transcriptional activation domain that activates expression of the gene downstream of the promoter.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2005},
month = {2}
}
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