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Title: Ultrahigh resolution multicolor colocalization of single fluorescent probes

Abstract

A novel optical ruler based on ultrahigh-resolution colocalization of single fluorescent probes is described. Two unique families of fluorophores are used, namely energy-transfer fluorescent beads and semiconductor nanocrystal (NC) quantum dots, that can be excited by a single laser wavelength but emit at different wavelengths. A novel multicolor sample-scanning confocal microscope was constructed which allows one to image each fluorescent light emitter, free of chromatic aberrations, by scanning the sample with nanometer scale steps using a piezo-scanner. The resulting spots are accurately localized by fitting them to the known shape of the excitation point-spread-function of the microscope.

Inventors:
; ;
Issue Date:
Research Org.:
Univ. of California, Oakland, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1175204
Patent Number(s):
6844150
Application Number:
09/925,100
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
G - PHYSICS G02 - OPTICS G02B - OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
B - PERFORMING OPERATIONS B82 - NANOTECHNOLOGY B82Y - SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES
DOE Contract Number:  
AC03-76F00098
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY

Citation Formats

Weiss, Shimon, Michalet, Xavier, and Lacoste, Thilo D. Ultrahigh resolution multicolor colocalization of single fluorescent probes. United States: N. p., 2005. Web.
Weiss, Shimon, Michalet, Xavier, & Lacoste, Thilo D. Ultrahigh resolution multicolor colocalization of single fluorescent probes. United States.
Weiss, Shimon, Michalet, Xavier, and Lacoste, Thilo D. Tue . "Ultrahigh resolution multicolor colocalization of single fluorescent probes". United States. https://www.osti.gov/servlets/purl/1175204.
@article{osti_1175204,
title = {Ultrahigh resolution multicolor colocalization of single fluorescent probes},
author = {Weiss, Shimon and Michalet, Xavier and Lacoste, Thilo D.},
abstractNote = {A novel optical ruler based on ultrahigh-resolution colocalization of single fluorescent probes is described. Two unique families of fluorophores are used, namely energy-transfer fluorescent beads and semiconductor nanocrystal (NC) quantum dots, that can be excited by a single laser wavelength but emit at different wavelengths. A novel multicolor sample-scanning confocal microscope was constructed which allows one to image each fluorescent light emitter, free of chromatic aberrations, by scanning the sample with nanometer scale steps using a piezo-scanner. The resulting spots are accurately localized by fitting them to the known shape of the excitation point-spread-function of the microscope.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2005},
month = {1}
}

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